[논문]Enhancement of Growth and Secondary Metabolite Biosynthesis: Effect of Elicitors Derived from Plants and Insects

Jeong Gwi-Taek; Park Don-Hee

doi:10.1007/bf02931186

Enhancement of Growth and Secondary Metabolite Biosynthesis: Effect of Elicitors Derived from Plants and Insects 원문보기

Biotechnology and bioprocess engineering : Bbe, v.10 no.1, 2005년, pp.73 - 77

Jeong Gwi-Taek (Engineering Research Institute, Chonnam National University) , Park Don-Hee (School of Biological Sciences and Technology, Chonnam National University, Faculty of Applied Chemical Engineering, Chonnam National University, Biotechnology Research Institute, Chonnam National University)

Abstract ▼ AI-Helper

Plant-derived natural products have been and will continue to be valuable sources. Elicitors have been employed to modify cell metabolism in order to enhance the productivity of useful metabolites in plant cell/tissue cultures. In this study, several elicitors were used to improve the productivity of useful metabolites and to reduce culture time for archiving high concentration in P. ginseng hairy root cultures. The addition of chitosan, chitosan oligosaccharide and alginate oligosaccharide to the culture of P. ginseng hairy roots caused growth to be inhibited with the increase in elicitor concentration. The usage of the chitosan elicitor and D-glucosamine caused a slight decrease in hairy root growth, whereas total ginseng saponin accumulated slightly with the increase in elicitor concentration. When gel beads were added to the culture medium at the initial period, hairy root growth was enhanced. The maximum growth was 1.35 times higher than that of the control at $1\%$ (w/v). Total ginseng saponin content decreased due to the addition of alginate beads. This would result in consistent diffusion of lower levels of calcium ions during the culture period that promotes biomass growth.

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가설 설정

4. However, total ginseng saponin content increased slightly with the increase in elicitor concentration. The total ginseng saponin productivity was enhanced to about 1.

제안 방법

For the experiments, the gel beads were added from a 0 to 5% (w/v) concentration into 250-mL flask cultures containing 100 mL of 1/2 MS medium and cultured for 21 days. Hairy roots were harvested and the biomass and metabolite content were determined.
In order to investigate the effect of agarose, alginic acid, agar and sodium alginate on growth and total ginseng saponin content, each compound was added to 0.1% (w/v) at the initial culture period. Alginate oligosaccharide and chitosan oligosaccharide were added from 0 to 0.
In this study, P. ginseng C.A. Meyer hairy root cultures, established by infecting with A. rhizogenes KCTC 2744, were used for improving the biosynthesis of a secondary metabolite by using several elicitors (chitosan, alginate, etc.).

이론/모형

The collected supernatant was used for total ginseng saponin analysis. Total ginseng saponin was measured by the Vanillin-H₂SO₄ colorimetric method [10], A calibration curve was established with a ginsenoside Re standard. Authentic ginsenoside Re was purchased from Sigma- Aldrich Co.

참고문헌 (12)

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상세보기
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원문보기 상세보기
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원문보기 상세보기
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상세보기
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상세보기
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상세보기
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상세보기
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상세보기
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상세보기
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상세보기
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