[논문]Enhanced Lovastatin Production by Solid State Fermentation of Monascus ruber

Xu Bao-Jun; Wang Qi-Jun; Jia Xiao-Qin; Sung Chang-Keun

doi:10.1007/bf02931187

Enhanced Lovastatin Production by Solid State Fermentation of Monascus ruber 원문보기

Biotechnology and bioprocess engineering : Bbe, v.10 no.1, 2005년, pp.78 - 84

Xu Bao-Jun (Department of Food Science and Technology, College of Agriculture and Biotechnology Chungnam National University, The pharmaceutical Institute, Dalian University) , Wang Qi-Jun (College of Food and Bioengineering, South China University of Technology) , Jia Xiao-Qin (Department of Food Science and Technology, College of Agriculture and Biotechnology Chungnam National University) , Sung Chang-Keun (Department of Food Science and Technology, College of Agriculture and Biotechnology Chungnam National University)

Abstract ▼ AI-Helper

The purpose of this study was to optimize the solid state cultivation of Monascus ruber on sterile rice. A single-level-multiple-factor and a single-factor-multiple-level experimental design were employed to determine the optimal medium constituents and to optimize carbon and nitrogen source concentrations for lovastatin production. Simultaneous quantitative analyses of the ${\beta}$-hydroxyacid form and ${\beta}$-hydroxylactone for of lovastatin were performed by the high performance liquid chromatography (HPLC) method with a UV photodiode-array (PDA) detector. The total lovastatin yield ($4{\sim}6\;mg/g$, average of five repeats) was achieved by adding soybean powder, glycerol, sodium nitrate, and acetic acid at optimized levels after 14 days of fermentation. The maximal yield of lovastatin under the optimal composition of the medium increased by almost 2 times the yield observed prior to optimization. The experimental results also indicated that the ${\beta}$-hydroxylactone form of lovastatin (LFL) and the ${\beta}$-hydroxyacid form of lovastatin (AFL) simultaneously existed in solid state cultures of Monascus ruber. while the latter was the dominant form in the middle-late stage of continued fermentation. These results indicate that optimized culture conditions can be used for industrial production of lovastatin to obtain high yields.

주제어

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제안 방법

However, the glucose and lactose additions had no significant effect on lovastatin production. Because glycerol contributed to the highest lovastatin level in the current tests, glycerol as an additive carbon source was further used for the singlefactor -multiple-level experimental test (Fig. 6A) to evaluate the optimal concentration level of glycerol. The results showed that the 1, 3, and 5% glycerol additions demonstrated significant differences (P<0.
ruber. First, two linear fit external standard curves of AFL and LFL were obtained by HPLC analysis. For samples analysis, after HPLC separation, 이uting substances were monitored with a photodiode array detector, Seven peaks were obtained with full baseline separation.
In the first part of the research, a screening work for lovastatin production was carried out by employing M. ruber strains. Lovastatin production was comparatively evaluated on 14-day-old cultures grown on steamed rice additive with a base medium as described above.
The statistical analysis software SPSS was used for all data process. The significance of differences between the control group and the optimization treatment group means was determined by one-way AN OVA with Post Hoc Tests. P values less than 0.

대상 데이터

The quantitative analyses of lovastatin were carried out by the Beckman 너PL© system. It was System Gold ® (Beckman, USA) equipped with a 128 Solvent Module, a 168 Detector, a 7725 auto sampler and an on-line de- gassin응 instrument. An ODS column (250 x 4.
, Ansan, Korea), and solvents used for spectroscopy were of spectroscopic grade. Trifluoroacetic (TFA) was purchased from Fluka (Buchs, Switzerland). HPLC grade water was prepared using a compact ultra-pure water system (Compact Co.

이론/모형

The quantitative analyses of lovastatin were carried out by the Beckman 너PL© system. It was System Gold ® (Beckman, USA) equipped with a 128 Solvent Module, a 168 Detector, a 7725 auto sampler and an on-line de- gassin응 instrument.

성능/효과

In conclusion, the production of lovastatin was influenced by the compositions and concentration levels of medium constituents. The lovastatin yield increased significantly through the addition of soybean powder, glycerol, sodium nitrate under optimal concentration levels.
05 mg/g, with significant differences between the experiment groups. Soybean powder contributed the highest lovastatin yield, compared with defatted soybean powder (DSBP) and mixture feed additive (FASBP) (contained defatted soybean powder), and differences reached to a significant level (P<0.05). Therefore, soybean powder was used as the basic additive substrate for the optimizing experiments.
Taking the above- mentioned factors into account, acetic acid was considered an important additive substance to use for the experimental test. The data indicated that 0.1, 0.2, 0.3, and 0.4% additions of acetic acid significantly increased total lovastatin production greater than the control group production (without an acetic acid addition), while 0.03, and 0.06% additions of acetic acid decreased lovastatin production significantly (Fig. 4).
In order to further explore the optimal soybean powder additive proportion, a single- factor-multiple-level experiment of soybean powder was done. The results (Fig. 3) showed that a 10% addition, a 14% addition (as the control), and a 20% addition could result in high level lovastatin yields: yields ranging from 3.33 to 4.02 mg/g. These results were significantly different from those at other levels (0, 1, and 5%), and this fact further verified that soybean powder is an important additive substrate for the SSF of M.
ruber. The results also illustrated that the soybean powder addition at the 20% level generated the highest yield among the present experiment levels, and lovastatin yields at this level were significantly different from the control group yield.
6B) to evaluate the optimal concentration level of sodium nitrate. The results showed that all of the various levels of the sodium nitrate additions (0.05, 0.1, 0.2, 0.3, 0.5%) generated significant differences (P<0.05) from the control group (without sodium nitrate addition). Among the sodium nitrate addition tests at different levels, the 0.
6A) to evaluate the optimal concentration level of glycerol. The results showed that the 1, 3, and 5% glycerol additions demonstrated significant differences (P<0.05) from the control group (without glycerol addition), but no significance was found in the 10% glycerol addition tests. Among the glycerol addition tests, the 3% glycerol addition achieved the highest lovastatin production and with a significant difference (P<0.
For the different nitrogen source tests, the organic nitrogen sources, such as yeast extract, and peptone, and the inorganic nitrogen sources such as sodium nitrate, and ammonium sulfate were used for evaluation. The results showed that the organic nitrogen source, yeast extract, and the inorganic nitrogen source, sodium nitrate, achieved relatively highter lovastatin productions than the control (without the nitrogen addition test) and other nutrient sources (Fig. 5B) with significance. No significance was found in the peptone and ammonium sulfate addition tests.

후속연구

, it is not very clear as to the effect of acetic acid on lovastatin production. In this respect, additional studies on biosynthesis mechanisms are needed as a basis for further research.

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