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Abstract

In order to investigate central metabolic changes in Beijerinckia indica, cells were grown on different carbon sources and intracellular flux distributions were studied under varying concentrations of nitrogen. Metabolic fluxes were estimated by combining material balances with extracellular substrate uptake rate, biomass formation rate, and exopolysaccharide (EPS) accumulation rate. Thirty-one metabolic reactions and 30 intracellular metabolites were considered for the flux analysis. The results revealed that most of the carbon source was directed into the Entner-Doudoroff pathway, followed by the recycling of triose-3-phosphate back to Hexose­6-phosphate. The pentose phosphate pathway was operated at a minimal level to supply the precursors for biomass formation. The different metabolic behaviors under varying nitrogen concentrations were observed with flux analysis.

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이 논문을 인용한 문헌 (2)

  1. Wu Jian-Rong ; Son Jeong Hwa ; Kim Ki Myong ; Nam Soo-Wan ; Lee Jin-Woo ; Kim Sung-Koo 2005. "Optimization of Heteropolysaccharide-7 Production by Beijerinckia Indica" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 33(2): 117~122 
  2. Jin, Il-Hyuck ; Jung, Dae-Young ; Son, Chang-Woo ; Kim, Sung-Koo ; Gao, Wa ; Chung, Chung-Han ; Lee, Jin-Woo 2011. "Enhanced Production of Heteropolysaccharide-7 by Beijerinckia indica HS-2001 in Repeated Batch Culture with Optimized Substitution of Culture Medium" Biotechnology and bioprocess engineering, 16(2): 245~255 

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