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제안 방법
- In this paper, Beijerinckia indica was cultivated in media with different carbon sources and a high or low nitrogen content for EPS fermentation. Metabolic flux was calculated to investigate the flux distribution in the central metabolism of the strain and metabolic changes under different nitrogen conditions. The Pentose Phospate pathway (PP), the semi- Embden-Meyerhof-Parnas (EMP) pathway, the Entner Doudoroff (ED) pathways, the Tricarboxylic acid (TCA) cycle, and EPS synthesis were used in the proposed metabolic network for metabolic flux analysis.
이론/모형
- The residual glucose concentration was determined by the phenol sulfuric acid method [9]. The ammonia was determined spectrophotometrically by the indophenol blue method 이. The nitrate was determined spectrophotometrically by the method by Cataldo et al.
- To determine biomass, culture broths were centrifuged, the resultant pellet was washed with distilled water, and dry cell weight was measured after drying at 105℃. The residual glucose concentration was determined by the phenol sulfuric acid method [9]. The ammonia was determined spectrophotometrically by the indophenol blue method 이.
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