$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Inhibitory Effect of an Entomopathogenic Bacterium, Xenorhabdus nematophila, on the Release of Arachidonic Acid from the Membrane Preparation of Spodoptera exigua

Journal of asia-pacific entomology v.8 no.1 , 2005년, pp.61 - 67  
Abstract

It has been suggested that an entomopathogenic bacterium, Xenorhabdus nematophila, exerts its pathogenicity against beet armyworm, Spodoptera exigua, by inhibiting phospholipase $A_2\;(PLA_2)$ to shut down release of arachidonic acid, which is the primary substrate for eicosanoid biosynthetic pathway. This research aimed to prove the inhibitory hypothesis of X. nematophila on $PLA_2$ by directly quantifying arachidonic acid released from natural biological membrane. For determining optimal membrane preparation, fatty acid compositions from different tissues (hemocyte, fat body, and gut) were analyzed using gas chromatography in S. exigua. These tissue membranes showed different compositions in fatty acids, but possessed significant levels of arachidonic acid, amounting to ${\approx}\;0.02\%$ among all fatty acids. Also, all cytosolic fractions of three tissues possessed significant and specific $PLA_2$ activity because these $PLA_2$ extracts catalyzed release of arachidonic acid from the membrane preparation into medium and were inhibited by a specific $PLA_2$ inhibitor, dexamethasone. For massive assay, we chose fat body as a tissue source of $PLA_2$ and substrate membrane because of its relative abundance. The effect of fat body $PLA_2$ extract on release of arachidonic acid increased with its concentration and incubation time. The fat body $PLA_2$ was susceptible to dexamethasone (a specific $PLA_2$ inhibitor) and p-bromophenacyl bromide (a secretory type $PLA_2$ inhibitor), but not to methylarachidonyl fluorophosphate (a cellular type $PLA_2$ inhibitor). X nematophila significantly inhibited fat body $PLA_2$ activity, but did not after heat-killing. An organic extract of X. nematophila culture broth also inhibited the fat body $PLA_2$ activity. These results indicate that S. exigua possesses arachidonic acid on the membrane and $PLA_2$ in tissues and that X. nematophila releases $PLA_2$ inhibitor(s) and inhibits $PLA_2$, which is susceptible to a secretory type $PLA_2$ inhibitor.

  

참고문헌 (27)

  1. Gho, H.G., S.G. Lee, K.M. Choi and J.H. Kim. 1990. Simple mass-rearing of beet armyworm, Spodoptera exigua, on an artificial diet. Korean J. Appl. Entomol. 29: 180-183 
  2. Park, Y. and Y. Kim. 2000. Eicosanoids rescue Spodoptera exigua infected with Xenorhabdus nematophilus, the symbiotic bacteria to the entomopathogenic nematode Steinernema carpocapsae. J. Insect Physiol. 46: 1469-1476? 
  3. Park, Y. and Y. Kim. 2003.Xenorhabdus nematophilus inhibits p-bromophenacyl bromide(BPB)-sensitive PLA2 of Spodoptera exigua. Arch. Insect Biochem. Physiol. 54: 134-142 
  4. Ribeiro, N.A., B. Duvic, P. Oliveira, A. Givaudan, F. Pahla and N. Simoes. 1999. Insect immunity effects of factors producedby a nematobacterial complexon immunocompetent cells. J. Insect Physiol. 45: 677-685 
  5. Stanley-Samuelson, D.W. 1994. Assessing the significance of prostaglandins and other eicosanoids in insect physiology. J. Insect Physiol. 40: 3-11 
  6. Akhurst, R.J. 1980. Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis. J. Gen. Microbiol. 121: 303-309 
  7. Schleusener, D.R. and D.W. Stanley-Samuelson. 1996. PhospholipaseA2 in hemocytesof the tobacco hornworm, Manduca sexta. Arch. Insect Biochem. Physiol. 33: 63-74 
  8. Cho, S. and Y. Kim. 2004. Hemocyte apoptosis induced by entomopathogenic bacteria, Xenorhabdus and Photorhabdus, in Bombyx mori. J. Asia-Pacific Entomol. 7: 195-200 
  9. Akhurst, RJ. and G.B. Dunphy. 1993. Tripartite interaction between symbiotically associated entomopathogenic bacteria, nematodes and their insect hosts. pp. 1-24, in Parasites and pathogens of insect, Vol. 1, Eds. Beckage, N.E., S.N. Thompson and B.A. Federici. Academic Press, New York 
  10. Park, Y., Y. Kim and Y. Lee. 1999. Identification and characterization of a symbiotic bacterium associated with Steinernema carpocapsae in Korea. J. Asia-Pacific Entomol. 2: 105-111 
  11. Gillespie, J.P., M.R. Kanost and T. Trenczek. 1997. Biological mediators of insect immunity. Annu. Rev. Entomol. 42: 611-643 
  12. SAS Institute Inc. 1989. SAS/STAT user's guide. SAS Institute Inc., Cary, North Carolina 
  13. Carlsson, A., P. Engstom, E.T. Paiva and H. Bennich. 1991. Attacin, an antibacterial protein of Hyalophora cecropia, inhibits synthesis of outer membrane proteins of Escherichia coli by interfering with omp gene transcription. Insect. lmmunol. 59: 3040-3045 
  14. Caldas, C., A. Cherqui, A. Pereira and A. Simoes. 2002. Purification and characterization of an extracellular protease from Xenorhabdus nematophila involved in insect immunosuppresion. Appl. Environ. Microbiol. 68: 1297-1304 
  15. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye finding. Anal. Biochem. 72: 248-254 
  16. Mandato, C.A., W.L. Diehl-Jones, S.J. Moore and R.G.H. Downer. 1997. The effects of eicosanoid biosynthesis inhibitors on prophenoloxidase activation, phagocytosis and cell spreading in Galleria mellonella. J. Insect Physiol. 43: 1-8 
  17. Ji, D. and Y. Kim. 2004. An entomopathogenic bacterium, Xenorhabdus nematophila, inhibits the expression of an antibacterial peptide, cecropin, of the beet armyworm, Spodoptera exigua. J. Insect Physiol. 50: 489-496 
  18. Forst, S., B. Dowds, N. Boemare and E. Stackebrandt. 1997. Xenorhabdus and Heterorhabdus spp.: bugs that kill bugs. Annu. Rev. Microbiol. 51: 47-72 
  19. Six, D.A. and E.A. Dennis. 2000. The expanding superfamily of phospholiase A2 enzyme: classification and characterization. Biochim. Biophys. Acta 1488: 1-19 
  20. Dunphy, D.B. and J.M. Webster. 1991. Antihemocytic surface components of Xenorhabdus nematophilus var. dutki and their modification by serum of nonimmune larvae of Galleria mellonella. J. Invertebr. Pathol. 58: 40-51 
  21. Stanley-Samuelson, D.W. and RH. Dadd. 1983. Polyunsaturated fatty acids: patterns of occurrence in insects. Insect Biochem. 14: 321-327 
  22. Taketo, M.M. and M. Sonoshita. 2002. Phospholipase A2 and apoptosis. Biochim. Biophys. Acta 1585: 72-76 
  23. Park, Y., Y. Kim, S.M. Putnam and D.W. Stanley. 2003. The bacterium, Xenorhabdus nematophilus depresses nodulation reactions to infection by inhibiting eicosanoid biosynthesis in tobacco hornworms, Manduca sexta. Arch. Insect Biochem. Physiol. 52: 71-80 
  24. Tanada, Y. and H.K. Kaya. 1993. Insect pathology. Academic Press. San Diego, California 
  25. Faye, I. 1990. Acquired immunity in insects: the recognition of nonself and the subsequent onset of immune protein genes. Res. Immunol. 141: 927-932 
  26. Stanley, D.W. 2000. Eicosanoids in invertebrate signal transduction systems. 292pp. Princeton University Press. New Jersey 
  27. Dowds, B.C.A. and A. Peters. 2002. Entomopathogenic nematology. pp. 79-98, in Virulence mechanisms, Ed. Gaugler, R. CABI Publishing, UK 

이 논문을 인용한 문헌 (2)

  1. 2006. "" Journal of asia-pacific entomology, 9(1): 31~36 
  2. Eom, Seonghyeon ; Park, Jiyeong ; Kim, Kunwoo ; Kim, Yonggyun 2014. "Effect of Cellular Phospholipase A2 Inhibition on Enhancement of Bt Insecticidal Activity" 한국응용곤충학회지 = Korean journal of applied entomology, 53(3): 271~280 

원문보기

원문 PDF 다운로드

  • 원문 PDF 정보가 존재하지 않습니다.

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일