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논문 상세정보

Abstract

Plant chitinases have been known as pathogenesis-related (PR) proteins, but recent studies suggest that they play functional roles during normal plant growth and development. We previously isolated two cDNA clones encoding endochitinases, EuNOD-CHT1 and -CHT2, from the root nodules of Elaeagnus umbellata. These genes show differential expression patterns, with the EuNOD-CHT1 gene being active in the root nodules and meristems, while EuNOD­CHT2 is preferentially expressed in the infected cells of those nodules. To elucidate the functional roles of these two endochitinases, we have now constitutively expressed each gene in a heterologous plant system, Arabidopsis thaliana. Stable inheritance and expression of the transgenes were confirmed by genomic Southern hybridization and RT-PCR. Our transgenic plants did not differ morphologically from the wild types. However, constitutive expression of EuNOD-CHT1 and -CHT2 in Arabidopsis resulted in increased resistance against a fungal pathogen, Botrytis cinerea, but not against a bacterial agent, Pseudomonas syringae pv. Tomato DC3000. Expression levels were enhanced by both wounding and jasmonic acid treatments (for EuNOD-CHT1), or by jasmonic acid only (for EuNOD-CHT2). These data suggest that EuNOD-CHTl and -CHT2 primarily play defensive roles during root nodule development in E. umbellata.

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이 논문을 인용한 문헌 (1)

  1. 2006. "" Journal of plant biology = 식물학회지, 49(6): 413~420 

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