Abstract

A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5' ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about $41\%$ of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs $(15\%)$ were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising $1\%$ of the ESTs. Genes related to energy and protein fates constituted the second $(10\%)$ and third $(9\%)$ largest groups, respectively. Finally, $3\%$ of the ESTs were assigned to transcription, and $2\%$ to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response.

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