[논문]Low Expression Profiles of Heat Stress-Related Genes in Capsicum annuum

Ashrafuzzaman M.; Oh S.June; Hong Choo Bong

doi:10.1007/bf03030567

Low Expression Profiles of Heat Stress-Related Genes in Capsicum annuum

Journal of plant biology = 식물학회지, v.48 no.1, 2005년, pp.85 - 95

Ashrafuzzaman M. (Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University) , Oh S.June (Department of Pharmacology, Pharmacogenomics Research Center, College of Medicine, Inje University) , Hong Choo Bong (Department of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University)

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A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5' ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about $41\%$ of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs $(15\%)$ were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising $1\%$ of the ESTs. Genes related to energy and protein fates constituted the second $(10\%)$ and third $(9\%)$ largest groups, respectively. Finally, $3\%$ of the ESTs were assigned to transcription, and $2\%$ to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response.

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Low Expression Profiles of Heat Stress-Related Genes in Capsicum annuum

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