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논문 상세정보

Development of an Agrobacterium-Mediated Transformation System for Regenerating Garland Chrysanthemum (Chrysanthemum coronarium L


Although efficient shoot regeneration and selection are essential for genetic transformation mediated by Agrobacterium, success has been limited with the garland chrysanthemum (Chrysanthemum coronarium L.). In this study, we developed a useful protocol for shoot regeneration with leaf disk explants. The optimal concentrations of NM and BA were 0.2 mg $L^{-1}$ and 0.5 mg $L^{-1}$, respectively. To optimize the selection system for regenerating plants from genetically transformed tissues, we tested the effects of four antibiotics (kanamycin, hygromycin, carbenicillin, and cefotaxime). Among them, 5mg $L^{-1}$ hygromycin proved adequate as a selectable marker, whereas 500mg $L^{-1}$ carbenicillin was effective in eliminating excessive Agrobacterium after co-cultivation. Transgenic plants were obtained by first co-culturing garland chrysanthemum leaf disks with A. tumefaciens strain EHA105, which harbors plasmid pRCVII containing the hygromycin resistance (hpt) and $\beta-glucuronidase$ (GUS) genes. After the transgenic plants were confirmed via Southern analysis, they were rooted in soil and appeared phenotypically normal. Our report is the first to describe the optimum conditions for producing transgenic plants of this species.

참고문헌 (18)

  1. Fukai S, de Jong J, Rademaker W (1995a) Agrobacterium-mediated genetic transformation of chrysanthemum. Acta Horticulturae 392: 147-152 
  2. Kim HS (2002) Development and application of plant transformation vector for rescue cloning. M.S. thesis, Kyung Hee University, Yongln 
  3. Schroder M, Dixelius C, Rahlen L, Glimelius K (1991) Transformation of Brassica napus by using the aadA gene as selectable marker and inheritance studies of the marker genes. Physiol Plant 92: 37-46 
  4. Sharma KK, Bhowani SS, Thorpe TA (1990) Factors affect high frequency differentiation of shoot and roots from cotyledon explants of Brassica juncea (L.) C. Plant Sci 66: 247-253 
  5. de Block M, Brouwer D, Tenning P (1989) Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol 91: 694-701 
  6. Ledger SE, Deroles SC, Given NK (1991) Regeneration and Agrobacterium-mediated transformation of chrysanthemum. Plant Cell Rep 10: 195-199 
  7. Annadana S, Rademaker W, Ramanna M, Udayakumar M, de Jong J (2000) Response of stem explants to screening and explant source as a basis for methodical advancing of regeneration protocols for chrysanthemum. Plant Cell Tiss Org Cult 62: 47-55 
  8. Saito K, Yamazaki M, Murakashi I (1992) Transgenic medical plants: Agrobacterium-mediated foreign gene transfer and production of secondary metabolites. J Natural Products 55: 149-162 
  9. Hachey JE, Sharama KK, Moloney MM (1991) Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro. Plant Cell Rep 9: 549-554 
  10. Macouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76: 815-829 
  11. Renou JP, Brochard P, Jalouzot R (1993) Recovery of transgenic chrysanthemum (Dendranthema grandiflora Tzvelev) after hygromycin resistance selection. Plant Sci 89: 185-197 
  12. Gonzalez A, Jimenez A, Vazquez D, Davis J, Schindler D (1978). Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta 521: 459-469 
  13. Hauptmann RM, Vasil V, Ozias-Atkins PM, Tabaeizadeh Z, Rogers SG, Fraley RT, Horsch RB, Vasil IK (1988) Evaluation of selectable markers for obtaining stable transfermants in the Cramineae. Plant Physiol 86: 602-606 
  14. Fukai S, de Jong J, Rademaker W (1995b) Efficient genetic transformation of chrysanthemum (Dendranthema grandiflorum Kitamura) using stem segments. Breeding Sci 45: 179-184 
  15. Lowe JM, Davey MR, Power JB, Blundy KS (1993) A study of some factors affecting Agrobacterium tumefaciens and plant regeneration of Dendranthema grandiffora Tzvelev (syn. Chrysanthemum morifolium Ramat). Plant Cell Tiss Org Cult 33: 171-180 
  16. Mol JNM, Stuitje AR, Gerats A, van der Krol A, Jorgensen R (1989) Saying it with genes: Molecular flower breeding. Trends Biotech 7: 148-153 
  17. Cabanas MJ Vazquez D, Modolell J (1978) Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition. Eur J Biochem 87: 21-27 
  18. John MS, Moyer WJ, Margaret ED (1998) A regeneration and Agrobacterium-mediated transformation system for genetically diverse chrysanthemum cultivars. J Amer Soc Hort Sci 123: 189-194 

이 논문을 인용한 문헌 (3)

  1. 2007. "" Journal of plant biology = 식물학회지, 50(5): 577~585 
  2. 2007. "" Journal of plant biology = 식물학회지, 50(6): 626~631 
  3. 2009. "" Journal of plant biotechnology = 식물생명공학회지, 36(3): 275~280 


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