[논문]Development of an Agrobacterium-Mediated Transformation System for Regenerating Garland Chrysanthemum (Chrysanthemum coronarium L.)

Chung Kwi-Mi; Park Young-Doo

doi:10.1007/bf03030573

Development of an Agrobacterium-Mediated Transformation System for Regenerating Garland Chrysanthemum (Chrysanthemum coronarium L.)

Journal of plant biology = 식물학회지, v.48 no.1, 2005년, pp.136 - 141

Chung Kwi-Mi (Graduate School of Biotechnology, KyungHee University) , Park Young-Doo (Graduate School of Biotechnology, KyungHee University)

Abstract ▼ AI-Helper

Although efficient shoot regeneration and selection are essential for genetic transformation mediated by Agrobacterium, success has been limited with the garland chrysanthemum (Chrysanthemum coronarium L.). In this study, we developed a useful protocol for shoot regeneration with leaf disk explants. The optimal concentrations of NM and BA were 0.2 mg $L^{-1}$ and 0.5 mg $L^{-1}$, respectively. To optimize the selection system for regenerating plants from genetically transformed tissues, we tested the effects of four antibiotics (kanamycin, hygromycin, carbenicillin, and cefotaxime). Among them, 5mg $L^{-1}$ hygromycin proved adequate as a selectable marker, whereas 500mg $L^{-1}$ carbenicillin was effective in eliminating excessive Agrobacterium after co-cultivation. Transgenic plants were obtained by first co-culturing garland chrysanthemum leaf disks with A. tumefaciens strain EHA105, which harbors plasmid pRCVII containing the hygromycin resistance (hpt) and $\beta-glucuronidase$ (GUS) genes. After the transgenic plants were confirmed via Southern analysis, they were rooted in soil and appeared phenotypically normal. Our report is the first to describe the optimum conditions for producing transgenic plants of this species.

참고문헌 (18)

Plant Cell Tiss Org Cult S Annadana 62 47 2000 10.1023/A:1006483414260 Annadana S, Rademaker W, Ramanna M, Udayakumar M, de Jong J (2000) Response of stem explants to screening and explant source as a basis for methodical advancing of regeneration protocols for chrysanthemum. Plant Cell Tiss Org Cult 62: 47-55
Eur J Biochem MJ Cabanas 87 21 1978 10.1111/j.1432-1033.1978.tb12347.x Cabanas MJ, Vazquez D, Modolell J (1978) Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition. Eur J Biochem 87: 21-27

상세보기
Plant Physiol M Block de 91 694 1989 10.1104/pp.91.2.694 de Block M, Brouwer D, Tenning P (1989) Transformation ofBrassica napus andBrassica oleracea usingAgrobacterium tumefaciens and the expression of the bar andneo genes in the transgenic plants. Plant Physiol 91: 694-701

상세보기
Acta Horticulturae S Fukai 392 147 1995 10.17660/ActaHortic.1995.392.17 Fukai S, de Jong J, Rademaker W (1995a)Agrobacteriummediated genetic transformation of chrysanthemum. Acta Horticulturae 392: 147-152

상세보기
Breeding Sci S Fukai 45 179 1995 Fukai S, de Jong J, Rademaker W (1995b) Efficient genetic transformation of chrysanthemum (Dendranthema grandiflorum Kitamura) using stem segments. Breeding Sci 45: 179-184
Biochim Biophys Acta A Gonzalez 521 459 1978 10.1016/0005-2787(78)90287-3 Gonzalez A, Jimenez A, Vazquez D, Davis J, Schindler D (1978) Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta 521: 459-469

상세보기
Plant Cell Rep JE Hachey 9 549 1991 10.1007/BF00232329 Hachey JE, Sharama KK, Moloney MM (1991) Efficient shoot regeneration ofBrassica campestris using cotyledon explants culturedin vitro. Plant Cell Rep 9: 549-554

상세보기
Plant Physiol RM Hauptmann 86 602 1988 10.1104/pp.86.2.602 Hauptmann RM, Vasil V, Ozias-Atkins PM, Tabaeizadeh Z, Rogers SG, Fraley RT, Horsch RB, Vasil IK (1988) Evaluation of selectable markers for obtaining stable transformants in the Gramineae. Plant Physiol 86: 602-606

상세보기
J Amer Soc Hort Sci MS John 123 189 1998 10.21273/JASHS.123.2.189 John MS, Moyer WJ, Margaret ED (1998) A regeneration and Agrobacterii/m-mediated transformation system for genetically diverse chrysanthemum cultivars. J Amer Soc Hort Sci 123: 189-194

상세보기
Kim HS (2002) Development and application of plant transformation vector for rescue cloning. M.S. thesis, Kyung Hee University, Yongln
Plant Cell Rep SE Ledger 10 195 1991 10.1007/BF00234294 Ledger SE, Deroles SC, Given NK (1991) Regeneration and Agrobacterium-mediated transformation of chrysanthemum. Plant Cell Rep 10: 195-199

상세보기
Plant Cell Tiss Org Cult JM Lowe 33 171 1993 10.1007/BF01983231 Lowe JM, Davey MR, Power JB, Blundy KS (1993) A study of some factors affectingAgrobacterium tumefaciens and plant regeneration ofDendranthema grandiflora Tzvelev (syn.Chrysanthemum morifolium Ramat). Plant Cell Tiss Org Cult 33: 171-180

상세보기
Theor Appl Genet SR Macouch 76 815 1988 10.1007/BF00273666 Macouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76: 815-829

상세보기
Trends Biotech JNM Mol 7 148 1989 10.1016/0167-7799(89)90025-5 Mol JNM, Stuitje AR, Gerats A, van der Krol A, Jorgensen R (1989) Saying it with genes: Molecular flower breeding. Trends Biotech 7: 148-153

상세보기
Plant Sci JP Renou 89 185 1993 10.1016/0168-9452(93)90127-L Renou JP, Brochard P, Jalouzot R (1993) Recovery of transgenic chrysanthemum (Dendranthema grandiflora Tzvelev) after hygromycin resistance selection. Plant Sci 89: 185-197

상세보기
J Natural Products K Saito 55 149 1992 10.1021/np50080a001 Saito K, Yamazaki M, Murakoshi I (1992) Transgenic medical plants: Agrobacterium-mediated foreign gene transfer and production of secondary metabolites. J Natural Products 55: 149-162

상세보기
Physiol Plant M Schroder 92 37 1991 10.1111/j.1399-3054.1994.tb06652.x Schroder M, Dixelius C, Rahlen L, Glimelius K (1991) Transformation ofBrassica napus by using theaadA gene as selectable marker and inheritance studies of the marker genes. Physiol Plant 92: 37-46

상세보기
Plant Sci KK Sharma 66 247 1990 10.1016/0168-9452(90)90210-F Sharma KK, Bhowani SS, Thorpe TA (1990) Factors affect high frequency differentiation of shoot and roots from cotyledon explants ofBrassica juncea (L.) C. Plant Sci 66: 247-253

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Development of an Agrobacterium-Mediated Transformation System for Regenerating Garland Chrysanthemum (Chrysanthemum coronarium L.)

Abstract ▼ AI-Helper

참고문헌 (18)

이 논문을 인용한 문헌

관련 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트