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논문 상세정보

Development of an Agrobacterium-Mediated Transformation System for Regenerating Garland Chrysanthemum (Chrysanthemum coronarium L

Abstract

Although efficient shoot regeneration and selection are essential for genetic transformation mediated by Agrobacterium, success has been limited with the garland chrysanthemum (Chrysanthemum coronarium L.). In this study, we developed a useful protocol for shoot regeneration with leaf disk explants. The optimal concentrations of NM and BA were 0.2 mg $L^{-1}$ and 0.5 mg $L^{-1}$, respectively. To optimize the selection system for regenerating plants from genetically transformed tissues, we tested the effects of four antibiotics (kanamycin, hygromycin, carbenicillin, and cefotaxime). Among them, 5mg $L^{-1}$ hygromycin proved adequate as a selectable marker, whereas 500mg $L^{-1}$ carbenicillin was effective in eliminating excessive Agrobacterium after co-cultivation. Transgenic plants were obtained by first co-culturing garland chrysanthemum leaf disks with A. tumefaciens strain EHA105, which harbors plasmid pRCVII containing the hygromycin resistance (hpt) and $\beta-glucuronidase$ (GUS) genes. After the transgenic plants were confirmed via Southern analysis, they were rooted in soil and appeared phenotypically normal. Our report is the first to describe the optimum conditions for producing transgenic plants of this species.

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이 논문을 인용한 문헌 (3)

  1. 2007. "" Journal of plant biology = 식물학회지, 50(5): 577~585 
  2. 2007. "" Journal of plant biology = 식물학회지, 50(6): 626~631 
  3. 2009. "" Journal of plant biotechnology = 식물생명공학회지, 36(3): 275~280 

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