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논문 상세정보

Research on the Allergic Potential of Insecticidal CrylAc Proteins of Genetically Modified Rice

Abstract

In Korea, different kinds of genetically modified (GM) crops are under development, including GM-rice expressing insecticidal crystal (Cry) proteins of Bacillus thuringiensis (Bt) modified to change a single amino acid. In this study, amino acid (aa) sequences of modified Cry proteins were compared to that of known allergens, and Cry proteins expressed in GM-rice were identified by using Cry protein specific polyclonal antibody. The antigen-antibody reactions were compared between GM and commercial rice to assess the allergic risk of Cry proteins. This analysis showed no known allergen to have more than 35% aa sequence homology with modified Cry proteins in Bt rice over an 80 aa window or to have more than 8 consecutive identical aa. Sera from allergic patients showed some IgE reactivity via immunoblotting and enzyme-linked immunosorbent assay (ELISA), although no differences were seen between GM and commercial rice. Based on these results we conclude that GM rice with modified Cry proteins has no differences in its protein composition or allergenicity relative to commercial rice.

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참고문헌 (24)

  1. Shaefer T, Kraemer U, Dockery D, Vieluf D, Behrendt H, Ring J. What makes a child allergic? Analysis of risk factors for allergic sensitization in preschool children from East and West Germany. Allergy Asthma Proc. 20: 23-27 (1999) 
  2. Yunginger JW, Sweeney KG, Sturner WQ, Giannandrea LA, Teigland JD, Bray M, Benson PA, York JA, Biedrzycki L, Squillace DL, Helm R.M. Fatal food-induced anaphylaxis. J. Am. Med. Asso. 260: 1450-1452 (1988) 
  3. Perr HA. Children and genetically engineered food: Potentials and problems. J. Pediatr. Gastr. Nutr. 35: 475-486 (2002) 
  4. Taylor S, Hefle SL. Will genetically modified foods be allergenic? J. Allergy Clin. Immun. 107: 765-771 (2001) 
  5. Astwood JD, Bannon GA, Dobert RL, Fuchs RL. Food biotechnology and genetic engineering. pp. 38-50. In: Food Allergy: Adverse Reactions to Foods and Food Additives. Metcalfe DD, Sampson HA, Simon RA (eds). Oxford, Blackwell Science, New York, NY, USA (2003) 
  6. Newell CA. Plant transformation technology. Developments and applications. Mol. Biotechnol. 16: 53-65 (2000) 
  7. Shin DW, Park SH, Woo GJ, Kim HY, Park CS. Case study for natural gene transfer from genetically modified food to food microorganisms. Food Sci. Biotechnol. 13: 342-346 (2004) 
  8. Bruce MC. Food safety evaluation of crops produced through biotechnology. J. Am. Coll. Nutr. 21: 166-173 (2002) 
  9. Aronson AI, Beckman W, Dunn P. Bacillus thuringiensis and related insect pathogens. Microbiol. Rev. 50: 1-24 (1986) 
  10. Whiteley HR, Schnepf HE. The molecular biology of parasporal crystal body formation in Bacillus thuringiensis. Annu. Rev. Microbiol. 40: 549-576 (1986) 
  11. Son DY, Lee BR, Shon DW, Lee KS, Ahn KM, Nam SY, Lee SI. Allergenicity change of soybean proteins by thermal treatment. Korean J. Food Sci. Technol. 32: 959-963 (2000) 
  12. Keum EH, Lee SI, Oh SS. Effect of enzymatic hydrolysis of 7S globulin, a soybean protein, on its allergenicity and identification of its allergenic hydrolyzed fragments using SDS-PAGE. Food Sci. Biotechnol. 15: 128-132 (2006) 
  13. King TP, Hoffman D, Lowenstein H, Marsh DG, Platts-Mills TAE, Thomas W. Allergen nomenclature. Int. Arch. Allergy Imm. 105: 224-233 (1994) 
  14. Elsayed S, Apold J. Immunochemical analysis of cod fish allergen M: locations of the immunoglobulin binding sites as demonstrated by the native and synthetic peptides. Allergy 38: 449-459 (1983) 
  15. Rothbard JB, Gefter ML. Interaction between immunogenic peptides and MHC proteins. Annu. Rev. Immunol. 9: 527-565 (1991) 
  16. Son DY, Lee SI. Comparison of the characteristics of the major allergen Mal d 1 according to apple varieties. Food Sci. Biotechnol. 10: 132-136 (2001) 
  17. Shibasaki M, Suzuki S, Nemoto H, Kuroume T. Allergenicity and lymphocyte-stimulating property of rice protein J. Allergy Clin. Immun. 64: 259-265 (1979) 
  18. Lownstein H. Quantitative immunoelectrophoretic methods as a tool for the analysis and isolation of allergens. Prog. Allergy 25: 1-62 (1978) 
  19. Matsuda T, Sugiyama M, Nakamura R, Torii S. Purification and properties of an allergenic protein in rice grain. Agric. Biol. Chem. 52: 1465-1470 (1988) 
  20. Urisu A, Yamada K, Masuda S, Komada H, Wada E, Kondo Y, Horiba F, Tsuruta M, Yasaki T, Yamada M. 16-kilodalton rice protein is one of the major allergens in rice grain extract and responsible for cross-allergenicity between cereal grains in the Poaceae family. Int. Arch. Aller. A. Imm. 96: 244-252 (1991) 
  21. Yamada M. Rice protein 16KD - a major allergen in rice grain extract. Arerugi 40: 1370-1376 (1991) 
  22. Nakase M, Usui Y, Alvarez-Nakase AM, Adachi T, Urisu A, Nakamura R, Aoki N, Kitajima K, Matsuda T. Cereal allergens: rice-seed allergens with structural similarity to wheat and barley allergens. Allergy 53: 55-57 (1998) 
  23. Ikezawa Z, Tsubaki K, Osuna H, Shimada T, Moteki K, Sugiyama H, Katumata K, Anzai H, Amano S. Usefulness of hypoallergenic rice (AFT-R 1) and analysis of the salt insoluble rice allergen molecule. Arerugi 48: 40-49 (1999) 
  24. Ikezawa Z, Ikebe T, Ogura H, Odajima H, Kurosaka F, Komatu H, Sase K, Suga C, Sugiuchi M, Suguro H. Mass trial of hypoallergenic rice (HRS-1) produced by enzymatic digestion in atopic dermatitis with suspected rice allergy. HRS-1 Research Group Acta. Derm. Venereol. Suppl. Stockh. 176: 108-112 (1992) 

이 논문을 인용한 문헌 (3)

  1. 2007. "" Food science and biotechnology, 16(6): 1011~1017 
  2. 2007. "" Food science and biotechnology, 16(1): 67~70 
  3. 2008. "" Food science and biotechnology, 17(2): 357~361 

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