초록

본 연구의 목적은 기존 상용화된 근관 봉함재인 레진계 봉함재 (AH 26, AH Plus), 산화 아연 유지놀계 봉함재(Tubliseal EWT Pulp Canal Sealer EWT), 수산화 칼슘계 봉함재 (Sealapex), 기존의 인산삼칼슘계 봉함재(Sankin Apatite type I, II, III)와 새로이 개발된 인산칼슘계 근관 봉함재 (CAPSEAL I, CAPSEAL II)의 세포독성과 유전독성을 비교 평가하고자 하였다. MTT test를 통해 세포독성을 평가하였으며 미생물을 이용한 복귀돌연변이 시험 (Ames test)으로 유전독성을 평가하였다. 이 연구의 결과는 아래와 같다. 1. 즉시군이 24시간군에 비해 MTT assay에서 세포독성이 높게 나타났다 (p<0.001). 2. 즉시군에서 CAPSEAL I과 CAPSEAL II는 AH 26, AH Plus, Tubliseal EWT Pulp Canal Sealer EWT, Sealapex와 SARCS II 보다 낮은 세포독성을 보였다 (p<0.01). 3. 즉시군에서 AH 26은 TA98과 TA100에 각각 S9 fraction을 처리한 경우와 그렇지 않은 경우 모두 유전독성을 나타냈으며, AH Plus 또한 TA100에 S9 fraction을 처리한 경우와 그렇지 않은 경우 유전독성을 나타냈다. 4. 즉시군에서 Tubliseal EWT, Pulp Canal Sealer EWT Sealapex가 TA100균주에 S9 fraction을 처리하였을 때 유전독성 양성반응이 나타났으며, 그 외의 경우는 모두 음성반응을 나타냈다. 5. 24시간군에서는 SARCS II가 TA98균주에서 S9 fraction처리했을 때와 처리하지 않았을 때 모두 유전독성이 나타났고, AH 26은 TA98에 S9 fraction을 처리하였을 때 유전독성이 나타났다 그 외의 경우는 모두 음성반응을 보였다. 6. CAPSEAL I과 CAPSEAL II는 유전독성에서 모두 음성반응을 나타냈다. 7. CAPSEAL I과 CAPSEAL II 두 근관봉함재 간에는 세포독성실험과 유전독성실험에서 즉시군과 24시간군 모두에서 통계학적으로 유의할 만한 차이를 보이지 않았다.

Abstract

The purpose of this study was to compare the cytotoxicity by MTT test and genotoxicity by Ames test of new calcium phosphate-based root canal sealers (CAPSEAL I, CAPSEAL II) with commercially available resin-based sealers (AH 26, AH Plus) , zinc oxide eugenol-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT), calcium hydroxide-based sealer (Sealapex), and tricalcium phosphate based sealers (Sankin Apatite Root Canal Sealer I, II, III). According to this study, the results were as follows : 1. The extracts of freshly mixed group showed higher toxicity than those of 24 h set group in MTT assay (p<0.001). 2. CAPSEAL I and CAPSEAL II were less cytotoxic than AH 26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT Sealapex and SARCS II in freshly mixed group (p<0.01). 3. AH 26 in freshly mixed group showed mutagenicity to TA98 and TA100 with and without S9 mix and AH Plus extracts also were mutagenic to TA100 with and without S9 mix. 4. Tubliseal EWT, Pulp Canal Sealer EWT and Sealapex in freshly mixed group were mutagenic to TA100 with S9 mix. 5. Among those of 24 h set groups the extracts of SARCS II were mutagenic to TA98 with and without S9 mix and AH 26 showed mutagenic effects to TA98 with S9 mix. 6. No mutagenic effect of CAPSEAL I and CAPSEAL II was detected. 7. There is no statistically significant difference between CAPSEAL I and CAPSEAL II at MTT assay and Ames test in both freshly mixed group and 24 h set group.

주제어

#근관 봉함재   #세포독성   #유전독성   #생체적합성   #인산칼슘  

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참고문헌 (46)

1. Mittal M, Chandra S, Chandra S. Comparative tissue toxicity evaluation of four endodontic sealers. J Endod 21:622-624, 1995 
2. Levin MP, Getter L, Adrian J, Cutright DE. Healing of periodontal defects with ceramic implants. J Clin Periodontol 1:197-205, 1974 
3. Sugawara A, Chow LC, Tagaki S, Chohayeb H. In vitro evaluation of the sealing ability of a calcium phosphate cement when used as a root canal sealer-filler. J Endod 16:162-165, 1990 
4. Sugawara A, Nishiyama M, Kusama K, Moro I, Nishimura S, Kudo I, Chow LC, Takagi S. Histopathological reactions of calcium phosphate cement. Dent Mater J 11:11-16, 1992 
5. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Method 65:55-63, 1983 
6. Spangberg L, Langeland K. Biologic effects of dental materials. I. Toxicity of root canal filling materials on HeLa cells in vitro. Oral Surg 35:402-414, 1973 
7. Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. An in vitro study of the cytotoxicity of two root canal sealers. J Endod 26:228-229, 2000 
8. Willershausen B, Briseno B, Schafer D, Schulze R. Cytotoxicity of root canal filling materials to three different human cell line. J Endod 26:703-707, 2000 
9. Kolokuris I, Beltes P, Economides N, Vlemmas I. Experimental study of the biocompatibility of a new glass ionomer root canal sealer (Ketac Endo). J Endod 22:395-398, 1996 
10. Briseno BM, Willershausen B. Root canal sealer cytotoxicity with human gingival fibroblast III. Calcium hydroxide-based sealers. J Endod 18:110-111, 1992 
11. Leyhousen G, Heil J, Reifferscheid G, Waldmann P, Gerutsen W. Genotoxicity and cytotoxicity of the epoxy resin based sealer AH Plus. J Endod 25:109-113, 1999 
12. Wright KJ, Barbosa SV, Araki K, Spangberg LS. In vitro antimicrobial and cytotoxic effects of Kri 1 paste and zinc oxide-eugenol used in primary tooth pulpectomies, Pediatr Dent 16:102-106, 1994 
13. Miyamoto Y, Ishikawa K, Takechi M, Yuasa M, Kon M, Nagayama M, Asaoka K. Non-decay type fast-setting calcium phosphate cement: setting behaviour in calf serum and its tissue response. Biomaterials 17:1429-1435, 1996 
14. Saidon J, He J, Zhu Q, Safavi K, Spangberg LS. Cell and tissue reactions to mineral trioxide aggregate and Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 95:483-489, 2003 
15. Brown WE, Chow LC. A new calcium phosphate setting cement. J Dent Res 69:672, 1990 
16. Krell KF, Wefel JS. Calcium phosphate cement root canal sealer-scanning electron microscopic analysis. J Endod 10:571-576, 1984 
17. Beltes P, Koulaouzidou E, Kolokuris I. Kortsaris AH. In vitro evaluation of the cytotoxicity of two glassionomer root canal sealers. J Endod 23:572-574, 1997 
18. Chohayeb H, Chow LC, Tsaknis PJ. Evaluation of calcium phosphate as a root canal sealer-filler material. J Endod 13:384-387, 1987 
19. Yuan H, Li Y, de Bruijn JD, de Groot K, Zhang X. Tissue responses of calcium phosphate cement: a study in dogs, Biomaterials 21:1283-1290, 2000 
20. Bilginer S, Esener T, Soylemezoglu F, Tiftik AM. The investigation of biocompatibility and apical microleakage of tricalcium phosphate based root canal sealers. J Endod 23:105-109, 1997 
21. Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin-, zinc oxide eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 35:153-158, 2002 
22. Pascon EA, Leonardo MR, Safavi K, Langeland K. Tissue reaction to endodontic materials: methods, criteria, assessment, and observations. Oral Surg 72:222-237, 1991 
23. LeGeros RZ. Calcium phosphate materials in restorative dentistry: a review. Adv Dent Res 2:164-180, 1988 
24. Chau JY, Hutter JW, Mork TO, Nicoll BK. An in vitro study of furcation perforation repair using calcium phosphate cement. J Endod 23:588-592, 1997 
25. Jacobson SM, von Fraunhofer JA. The investigation of microleakage in root canal therapy. An electrochemical technique. Oral Surg 42:817-823, 1976 
26. Shindo ML, Costantino PD, Friedman CD, Chow LC. Facial skeletal augmentation using hydroxyapatite cement. Arch Otolaryngol Head Neck Surg 119:185-190, 1993 
27. Spangberg LS, Barosa SV, Lavigne GD. AH 26 releases formaldehyde. J Endod 19:596-598, 1993 
28. Camilleri J, Montesin FE, Di Silvio L, Pitt Ford TR. The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use. Int Endod J 38:834-842, 2005 
29. Tai K, Huang F, Chang Y. Cytotoxic evaluation of root canal filling materials on primary human oral fibroblast cultures and a permanent hamster cell line. J Endod 27:571-573, 2001 
30. Fukase Y, Eanes ED, Tagaki S, Chow LC, Brown WE. Setting reaction and compressive strength of calcium phosphate cements. J Dent Res 69:1852-1856, 1990 
31. Matsumoto K, Inoue K, Matsumoto A. The effect of newly developed root canal sealers on rat pulp cells in primary culture. J Endod 15:60-67, 1989 
32. Yoshikawa M, Hayami S, Tsuji I, Toda T. Histopathological study of a newly developed root canal sealer containing tetracalcium-dicalcium phosphates and 1.0% chondroitin sulfate. J Endod 23:162-166, 1997 
33. Takechi M, Miyamoto Y, Ishikawa K, Toh T, Yuasa T, Nagayama M, Suzuki K. Initial histological evaluation of anti-washout type fast-setting calcium phosphate cement following subcutaneous implantation. Biomatedals 19:2057-2063, 1998 
34. Ribeiro DA, Duarte MA, Matsumoto MA, Marques ME, Salvadori DM. Biocompatibility in vitro tests of mineral trioxide aggregate and regular and white Portland cements. J Endod 31:605-607, 2005 
35. Beltes P, Koulaouzidou E, Kotoula V, Kortsaris AH. In vitro evaluating of the cytotoxicity of calcium hydroxide-based root canal sealers. Endod bent Traumatol 11:245-249, 1995 
36. Hong CY, Lin SK, Kok SH, Wong MY, Hong VC. Histological reaction to a newly developed calcium phosphate cement implanted in the periapical and periodontal tissues. J Formos Med Assoc 89:297-304, 1990 
37. Kim JS, Baek SH, Bae KS. In vivo study on the biocompatibility of newly developed calcium phosphatebased root canal sealers. J Endod 30:708-711, 2004 
38. Haikel Y, Wittenmeyer W, Bateman G, Bentaleb A, Allemann C. A new method for the quantitative analysis of endodontic microleakage. J Endod 25:172-177, 1999 
39. Segura JJ, Jmenez-Rubio A. Effect of eugenol on macropage adhesion in vivo to plastic surfaces. Endod Dent Traumatol 14:72-74, 1998 
40. Matsuya Y, Antonucci JM, Matsuya S, Takagi S, Chow LC. Polymeric calcium phosphate cements derived from poly(methyl vinyl ether-maleic acid). Dent Mater 12:2-7, 1996 
41. Koulaouzidou EA, Papazisis KT, Beltes P, Geromichalos GD, Kortsaris AH. Cytotoxicity of three resin-based root canal sealers: an in vitro evaluation. Endod Dent Traumatol 14:182-185, 1998 
42. Nikolaos E, Ioannis K. Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues. J Endod 21:122-127, 1995 
43. Ioannis K, Nikolaos E. In vivo comparison of the biocompatibility of two root canal sealers implanted into the subcutaneous connective tissue of rats. J Endod 24:82-85, 1998 
44. Araki K, Isaka H, Ishii T, Suda H. Excretin of $^{14}C$-formaldehyde distributed systemically through root canal following pulpectomy. Endod Dent Traumatol 9:196-199, 1993 
45. Gruninger SE, Siew C, Chow LC, O'Young A, Ts AO NK, Brown WE. Evaluation of the biocompatibility of a new calcium phosphate setting cement[abstract] J Dent Res 63:200, 1984 
46. Maron D, Ames B. Revised methods for the Salmonella mutagenicity test. Mutat Res 257:229-306, 1991 

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