$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Abstract

This study was conducted to compare the reproduction ability of the wild type boar and recombinant human erythropoietin (hEPO) transgenic boar semen. Ejaculated boar semen was analyzed by flow cytometry, Elisa and IVF methods. In experiment 1, flow cytometric analysis showed that the live sperm ratio of transgenic boar sperm significantly lower (P<0.05) than that of wild type boar after incubation at 20, 22, 24 and 26 hr. In experiment 2, the presence and levels of various cytokines (IL-6, IL-10 and $TNF-{\alpha}$) to related animal reproduction in the seminal and blood plasma were examined using specific enzyme immunoassay. There was no significant difference between both groups. In experiment 3, the fertilizing capacity and developmental ability of both boar sperm were compared. The transgenic boar sperm had a significantly low capacity of penetration, sperm-zona binding, embryo development, and blastocyst formation compared to wild type sperm (P<0.05). These results suggest that transgenic boar sperm harboring hEPO gene has low sperm viability than wild type boar, and it is a reason to decrease of fertility and litter size.

참고문헌 (36)

  1. Abeydeera LR, Wang WH, Cantley TC, Rieke A, Murphy CN, Prather RS, Day BN (2000): Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology 54:787-97 
  2. Burdon T, Sankaran L, Wall RJ, Spencer M, Hennighausen L (1991): Expression of a whey acidic protein transgene during mammary development. J Biol Chem 11: 6909-6914 
  3. Garner DL, Dobrinsky JR, Welch GR, Johnson LA (1996): Porcine sperm viability, oocyte fertilization and embryo development after staining spermatozoa with SYBR-14. Theriogenology 45:1103-1113 
  4. Evenson DP, Darzynkiewicz Z, Melamed MR (1980): Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131-1133 
  5. Erslev A (1953): Humoral regulation of red cell production. Blood 8:349-357 
  6. Feugang JM, Roover DE, Leonard S, Dessy F, Donnay I (2002): Kinetics of apoptosis in preimplantation bovine embryos produced in vitro and in vivo. Theriogenology 57:494 (Abstract) 
  7. Bookfor FR, Schwarz LK (1991): Effects of interleukin- 6, interleukin-2, and tumor necrosis factor Q on transferring release from Sertoli cells in culture. Endocrinology 129:256-262 
  8. Garner DL, Johnson LA (1995): Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53:276-284 
  9. Gillan L, Evans G, Maxwell WM (2005): Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63:445-457 
  10. Gjorret JO, Wengle J, King WA, Schellander K, Hyttel P (2002): Occurrence of apoptosis in bovine embryos reconstructed by nuclear transfer or derived in vivo. Theriogenology 57:495 (Abstract) 
  11. Hogan B, Costantini F, Lacy E (1994): Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, pp127-216 
  12. Eiserman J, Register KB, Striker RC, Collins JL (1989): The effect of tumor necrosis on human sperm motility in vitro. J Androl 10:270-274 
  13. Jelkmann W (1992): Erythropoietin: structure, control of production, and function. Physiol Rev 72: 449-489 
  14. Johnson LA, Aalbers JG, Gooton HJG (1988): Artificial insemination of swine: fecundity of boar semen stored in Beltsville TS (BTS), modified Modena (MM) or MR-A and inseminated on one, three and four days after collection. Zuchthygiene (Berl) 23:49-55 
  15. Lacombe C, Da Silva JL, Bruneval P, Camillery JP, Bariety J et al. (1988): Identification of tissues and cells producing erythropoietin in the anemic mouse. Contrib Nephrol 66:17-24 
  16. Levy RR, Cordonier H, Czyba JC, Goerin JF (2001): Apoptosis in preimplantation mammalian embryo and genetics. Int J Anat Embryol 106(Suppl):2101-2108 
  17. Limonta J, Pedraza A, Rodr Aguez A, Freyre M, Barral AM, Castro FO, Lleonart R, Garcia CA, Gavilondo JV, de la Fuente J (1995): Production of active anti-CD6 mouse/human chimeric antibodies in the milk of transgenic mice. Immunotechnology 1:107-113 
  18. Li S, Rosen JM (1994): Distal regulatory elements required for rat whey acidic protein gene expression in transgenic mice. J BioI Chem 269:14235-4243 
  19. Abeydeera, LR, Day BN (1997): In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium. Theriogenology 48:537-544 
  20. Harrison RAP (1997): Sperm plasma membrane characteristics and boar semen fertility. J Reprod Fertil (Suppl) 52:271-283 
  21. Mattioli M, Bacci ML, Galeati G, Seren E (1989): Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology 31:1201-1207 
  22. Miller LJ, Fischer KA, Goralnick SJ, Litt M, Burleson JA, Albertsen P, Kreutzer DL (2002): Interleukin- 10 levels in seminal plasma: implications for chronic prostatitis-chronic pelvic pain syndrome. J Urol 167:753-756 
  23. Skinner MK (1991): Cell-cell interactions in the testis. Endocrine Rev 12:45-77 
  24. Rajasekaran M, Hellstrom WJG, Naz RK, Sikka SC (1995): Oxidative stress and interleukins in seminal plasma dUring leukocytospermia. Fertil Steril 64: 166-171 
  25. Gruschwitz MS, Bresinschek R, Bresinschek HP (1996): Cytokine levels in the seminal plasma of infertile males. J Androl 14:158-163 
  26. Park JK, Lee YK, Lee P, Chung HJ, Kim S, Lee HG, Seo MK, Han JH, Park CG, Kim HT, Kim YK, Min KS, Kim JH, Lee HT, Chang WK (2006): Recombinant human erythropoietin produced in milk of transgenic pigs. J Biotechnol (in press) 
  27. Pierce CN, Larson DF (2005) Inflammatory cytokine inhibition of erythropoiesis in patients implanted with a mechanical circulatory assist device. Perfusion 20(2):83-90 
  28. Paleyanda RK, Zhang DW, Hennighausen L, McKnight RA, Lubon H (1994): Regulation of human protein C gene expression by the mouse WAP promoter. Transgenic Res 3:335-343 
  29. Kelly RW (1995): Immunosupressive mechanisms in semen : Implications for contraception. Hum Reprod 10:1686-1693 
  30. Tanphaichitr N, Smith J, Mongkolsirikieart S, Gradil C, Lingwood C (1993): Role of a gamete specific sulfoglycolipid-immobilizing protein on mouse sperm- egg binding. Dev BioI 156:164-175 
  31. Vera O, Vasquez LA, Munoz MG (2003): Semen quality and presence of cytokines in seminal fluid of bull ejaculates. Theriogenology 60:553-558 
  32. Wall RJ, Rexroad CE, Powell A, Shamay A, McKnight R, Hennighausen L (1996): Synthesis and secretion of the mouse whey acidic protein in transgenic sheep. Transgenic Res 5:67-72 
  33. Yamauchi-Takihara, Ihara Y, Ogata A, Yoshizaki K, Azuma J, Kishimoto T (1995): Hypoxic stress induces cardiac myocyte-derived interleukin-6. Circulation 91:1520-1524 
  34. Yoshida M, Ishizaki Y, Kawagishi H (1990): Blastocyst formation by pig embryos resulting from invitro fertilization of oocytes matured in vitro. J Reprod Fertil 88:1-8 
  35. Zhang J, Gao J (2004): Determination of IL-1beta, IL-4 and IL-10 contents in the seminal plasma of infertile patients and its clinical value. Zhonghua Nan Ke Xue 10:851-4 
  36. Zhu Y, Osterlundh I, Hulten F, Magnusson U (2004): Tumor necrosis factor-alpha, interleukin-6, serum amyloid A, haptoglobin, and cortisol concentrations in sows following intrarrlarrunary inoculation of Escherichia coli. Am J Vet Res 65:1434-1439 

이 논문을 인용한 문헌 (2)

  1. Bae, Seong-Hoon ; Hwang, Seong-Soo ; Yang, Byong-Chul ; Go, Yeoung-Kyu ; Kim, Dong-Hoon ; Im, Gi-Sun ; Choi, Hwa-Sik ; Jin, Dong-Il ; Yang, Boh-Suk ; Seong, Hwan-Hoo 2007. "Analysis of Semen Parameters, Sperm Activity, and Fertility of Somatic Cell Cloned Hanwoo Bulls" Reproductive & developmental biology = 한국동물번식학회지, 31(3): 139~143 
  2. 2008. "" Reproductive & developmental biology = 한국동물번식학회지, 32(2): 117~121 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일