• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide $(10{\mu}g/ml)$ for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to $6.25{\mu}M$ calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to $10{\mu}g/ml$ cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to $6.25{\mu}M$ calcium ionophore for 2 min with $10{\mu}g/ml$ cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.

참고문헌 (23)

  1. Liu CT, Chen CH, Cheng SP, Ju JC (2002): Parthenogenesis of rabbit oocytes activated by different stimuli. Anim Reprod Sci 70:267-276 
  2. Marcus GJ (1990): Activation of cumulus-free mouse oocytes. Mol Repred Dev 26:159-162 
  3. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH (2000): Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:505-509 
  4. Sirard MA, Florman HM, Leibfried-Rutledge ML, Barnes FL, First NL (1989): Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. BioI Reprod 40:1257-1263 
  5. Ware CB, Barnes FL, Maiki-Laurila M, First NL (1989): Age dependence of bovine oocytes activation. Gamete Res 22:265-275 
  6. Yamauchi N, Sasada H, Sugawara S, Nagai T (1996): Effect of culture conditions on artificial activation of porcine oocytes matured in vitro. Reprod Fertil 8:1153-1156 
  7. Ye J, Campbell KHS, Craigon J, Luck MR (2005): Dynamic changes in meiotic progression and improvement of developmental competence of pig oocytes in vitro by follicle stimulating hormone and cycloheximide. BioI Reprod 72:399- 406 
  8. Yi YJ, Park CS (2005): Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine. Anim Reprod Sci 86:297-304 
  9. Zhu J, Telfer EE, Fletcher J, Springbett A, Dobrinsky JR, De Sousa PA, Wilmut I (2002): Improvement of an electrical activation protocol for porcine oocytes. BioI Reprod 66:635-641 
  10. Bodart JF, Bechard D, Bertout M, Gannon J, Rousseau A, Vilain JP, Flament S (1995): Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis. Exp Cell Res 253:413-421 
  11. Im GS, Liangxue L, Zhonghua L, Yanhong H, David W, Aaron B, Randall SP (2003): In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenology 61:1125-1135 
  12. Petters RM, Wells KD (1993): Culture of pig embryos. J Reprod FertiI 48:61-73 
  13. Clarke HJ, Marui Y (1983): The induction of reversible and irreversible chromosome decondensation by protein synthesis inhibition of meiotic maturation of mouse oocytes. Dev BioI 97:291-301 
  14. Lan GC, Han D, Wu YG, Han ZB, Ma SF, Liu XY, Chang CL, Tan JH (2005): Effects of duration, concentration, and timing of ionomycin and 6-dimethylaminopurine (6-DMAP) treatment on activation of goat oocytes. Mol Reprod 71:380-388 
  15. Presicce GA, Tang X (1994b): Parthenogenetic development of bovine oocytes matured in vitro for 24h and activated by ethanol and cycloheximide. Mol Reprod 38:380-385 
  16. Yang X, Jiang S, Shi Z (1992): Improved activation by combined cycloheximide and electrical pulse treatment of bovine follicular oocytes matured in vitro for 23 - 24h. BioI Reprod (Suppl.) 46:117 (abstract) 
  17. Presicce GA, Tang X (1994a): Nuclear dynamics of parthenogenetic development of bovine oocytes matured in vitro for 20 and 40h and activated by combined by ethanol and cycloheximide treatment. Mol Reprod 37:61-68 
  18. Kim YS, Lee SL, Ock SA, Balasubramanian S, Choe SY, Rho GJ (2005): Development of cloned pig embryos by nuclear transfer following different activation treatments. Mol Reprod Dev 70:308-313 
  19. Galli C, Lagutina I, Vassiliev I, Duchi R, Lazzari G (2002): Comparison of microinjection (piezo-electric) and cell fusion for nuclear transfer success with different cell types in cattle. Cloning Stem Cells 4:189-196 
  20. Iwamoto M, Onishi A, Fuchimoto D, Somfai T, Takeda K, Tagami T, Hanada H, Noguchi J, Kaneko H, Nagai T, Kikuchi K (2005): Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage. Theriogenology 63:1277-1289 
  21. Jilek F, Huttelova R, Petr J, Holubova M, Rozinek J (2000): Activation of pig oocytes using calcium ionophore: effect of protein synthesis inhibitor cycloheximide. Anim Reprod Sci 63:101-111 
  22. Sirard MA, Whittingham DG, Molinaro M, Vivarelli E (1978): Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis. J Embryol Exp MorphoI 43:157-166 
  23. Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S (2002): Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. BioI Reprod 66:112-119 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일