본 연구는 록시스로마이신의 정맥주사 후 육계에서의 약물동태학적 특성을 조사한것으로, 이때 록시스로마이신은 체중당 20 mg/kg 용량으로 정맥주사하였다. 시간에 따라 채혈하여 혈장을 분리한 후 액체크로마토그래프/질량분석기를 이용하여 혈장내 록시스로마이신의 농도를 측정하였다. 혈장내 록시스로마신 농도-시간 그래프의 분석은 two-compartment open model을 적용하는 것이 가장 적합하였다. 육계에서의 록시스로마이신의 약물동태학적 부변수의 값은 다음과 같았다. 소실 반감기 =$5.83{\pm}1.79h$, 평균체류 =$6.33{\pm}0.32h$, 청소율 =$0.55{\pm}0.15L/h/kg$ 및 정상상태 분포용적 = $3.47{\pm}0.84L/kg$ 육계에서 정맥주사 후 록시스로마이신은 늦은 소실과 체내 고른 분포의 약물동태학적 특성을 나타내었다. 록시스로마이신의 육계에 적용할 때에는 약물제형, 최적 용량용법, 임상효과 및 반복투여에 대한 내성등의 연구가 추후 요구된다.
본 연구는 록시스로마이신의 정맥주사 후 육계에서의 약물동태학적 특성을 조사한것으로, 이때 록시스로마이신은 체중당 20 mg/kg 용량으로 정맥주사하였다. 시간에 따라 채혈하여 혈장을 분리한 후 액체크로마토그래프/질량분석기를 이용하여 혈장내 록시스로마이신의 농도를 측정하였다. 혈장내 록시스로마신 농도-시간 그래프의 분석은 two-compartment open model을 적용하는 것이 가장 적합하였다. 육계에서의 록시스로마이신의 약물동태학적 부변수의 값은 다음과 같았다. 소실 반감기 =$5.83{\pm}1.79h$, 평균체류 =$6.33{\pm}0.32h$, 청소율 =$0.55{\pm}0.15L/h/kg$ 및 정상상태 분포용적 = $3.47{\pm}0.84L/kg$ 육계에서 정맥주사 후 록시스로마이신은 늦은 소실과 체내 고른 분포의 약물동태학적 특성을 나타내었다. 록시스로마이신의 육계에 적용할 때에는 약물제형, 최적 용량용법, 임상효과 및 반복투여에 대한 내성등의 연구가 추후 요구된다.
The aim of the present study was to investigate the disposition pharmacokinetics of roxithromycin in broilers. Roxithromycin was administered at a single dose of 20 mg/kg body weight by intravenous (i.v.) routes. Plasma concentrations of roxithromycin were determined by liquid chromatography/mass sp...
The aim of the present study was to investigate the disposition pharmacokinetics of roxithromycin in broilers. Roxithromycin was administered at a single dose of 20 mg/kg body weight by intravenous (i.v.) routes. Plasma concentrations of roxithromycin were determined by liquid chromatography/mass spectrometry. After a single i.v. dose plasma concentrations were best fitted to a two-compartment open model. The values of the pharmacokinetic parameters after i.v. administration were: elimination half-life = $5.83{\pm}1.79h$, mean residence time = $6.33{\pm}0.32h$, total body clearance = $0.55{\pm}0.15L/h/kg$, and volume of distribution at steady state = $3.47{\pm}0.84L/kg$. The pharmacokinetic interpretation of roxithromycin after i.v. administration revealed that the drug was well distributed throughout the body in broilers and slowly eliminated. More studies for the application of roxithromycin against poultry disease are needed to establish a suitable pharmaceutical formulation, propose optimum dosage regimens, investigate clinical efficacy and study the tolerability of repeated doses.
The aim of the present study was to investigate the disposition pharmacokinetics of roxithromycin in broilers. Roxithromycin was administered at a single dose of 20 mg/kg body weight by intravenous (i.v.) routes. Plasma concentrations of roxithromycin were determined by liquid chromatography/mass spectrometry. After a single i.v. dose plasma concentrations were best fitted to a two-compartment open model. The values of the pharmacokinetic parameters after i.v. administration were: elimination half-life = $5.83{\pm}1.79h$, mean residence time = $6.33{\pm}0.32h$, total body clearance = $0.55{\pm}0.15L/h/kg$, and volume of distribution at steady state = $3.47{\pm}0.84L/kg$. The pharmacokinetic interpretation of roxithromycin after i.v. administration revealed that the drug was well distributed throughout the body in broilers and slowly eliminated. More studies for the application of roxithromycin against poultry disease are needed to establish a suitable pharmaceutical formulation, propose optimum dosage regimens, investigate clinical efficacy and study the tolerability of repeated doses.
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가설 설정
25, 0.5, 1, 2, 4, 8, 12, 36, and 48 h. The blood samples were collected in heparinized vacutainers (Becton Dickinson, Rutherford, USA), promptly centrifuged (room temperature, 10 min, 1000 x g) and the plasma was stored frozen at -70℃ until analysis.
제안 방법
plasma concentration-time data. The plasma concentration vs. time profiles after i.v. administration of roxithromycin was then analyzed by iterative nonlinear least squares regression analysis with equal weighting of the data using a WINNOLFN compartmental model program (Pharsight Corporation, California, USA). The estimation was performed with the use of the weighting model (1/C2 predicted) for the estimation of pharmacokinetic parameters.
대상 데이터
1~13 kg were used in this study. Broilers were housed in stainless steel cages with grate floors. The animals were acclimatized for 1 week before the experiment.
Twenty healthy broilers, aged 5 weeks, and weighing 1.1~13 kg were used in this study. Broilers were housed in stainless steel cages with grate floors.
이론/모형
The estimation was performed with the use of the weighting model (1/C2 predicted) for the estimation of pharmacokinetic parameters. Compartmental modeling was attempted for the i.v. data, and the best model was chosen using the Akaike's Information Criterion, together with visual inspection of the weighted residual plots (17).
693/p. The area under the plasma concentration vs. time curves for both the i.v. (AUCi.v. g) study was calculated by the method of trapezoids. The area under the first moment curve (AUMCo.
administration of roxithromycin was then analyzed by iterative nonlinear least squares regression analysis with equal weighting of the data using a WINNOLFN compartmental model program (Pharsight Corporation, California, USA). The estimation was performed with the use of the weighting model (1/C2 predicted) for the estimation of pharmacokinetic parameters. Compartmental modeling was attempted for the i.
The bacterial strains were grown in trypticase soy broth (Difco, USA) at 28℃ in air, resulting in approximately 5 x 109 CFU/ml. The minimum inhibitory concentrations (MICs) were determined by a broth microdilution method in trypticase soy broth (2). Serial two-fbld-dilutions of the antibiotics were inoculated with an overnight culture at a final inoculum of 106 CFU/mL.
성능/효과
In conclusion, the pharmacokinetic data obtained in this study indicate roxithromycin to be a qualified candidate for the treatment of bacterial infections in broilers. The large volume of distribution and slow elimination of the drug are excellent properties from a therapeutic point of view.
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