[논문]Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique

Kwon Eun-Young; Kim Young-Tae; Kim Dae-Eun

Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique 원문보기

Kwon Eun-Young (School of Mechanical Engineering, Yonsei University) , Kim Young-Tae (School of Mechanical Engineering, Yonsei University) , Kim Dae-Eun (Department of Mechanical Engineering, Yonsei University)

In this work, imaging and study of elastic property of the living cell was performed. The motivation of this work was to seek the possibility of exploiting Young's modulus as a disease indicator using Atomic Force Microscope (AFM) and also to gain fundamental understanding of cell mechanics for applications in medical nanorobots of the future. L-929 fibroblast adherent cell was used as the sample. Imaging condition in cell culturing media environment was done in very low speed ($20{\mu}m/ s$) compared to that in the ambient environment. For measuring the Young's modulus of the living cell, AFM indentation method was used. From the force-distance curve obtained from the indentation experiment the Young's modulus could be derived using the Hertz model. The Young's modulus of living L-929 fibroblast cell was $1.29{\pm}0.2$ kPa.

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가설 설정

range. First, the force is not linearly proportional to the distance since the cell is not perfectly elastic. In addition, the loading curve and the unloading curve do not correspond to each other as shown in Fig.

제안 방법

In this research the L-929 fibroblast image and Young's modulus were obtained in living state by exploiting the functions of an AFM.
The metal block helps in investigation of the sample cells on a cover glass by reflecting the light. The experiments were conducted in the cell culturing medium to keep the cells alive. The cell culturing medium was put into the Teflon dish and the sample cells on the metal block were immersed in the Teflon dish.
cells were done by utilizing an AFM. The experiments were done in the cell culturing medium to keep the cells alive.

대상 데이터

The cell line used for the experiment was L-929 mouse fibroblast (ATCC^®). The cells were cultured in the Dulbecco's modified Eagle's medium (DMEM) containing 10% Fetal Bovine Serum (FBS), 1% antibiotics.

이론/모형

The Hertz model was adopted to calculate the Young's modulus. According to the Hertz model, the applied load F, indentation depth d and Young's modulus E can be related by the following equation [7].

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Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique 원문보기

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Study on the Elastic Characteristics of Living Cells using Atomic Force Microscope Indentation Technique 원문보기

Abstract ▼ AI-Helper

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가설 설정

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