[논문]Adsorption of Nucleotides on <tex>${\\beta}$</tex>-Cyclodextrin Derivative Grafted Chitosan

Xiao Jian-Bo; Yu Hong-Zhu; Xu Ming; Chen Xiao-Qing

doi:10.1007/bf03219108

Adsorption of Nucleotides on ${\\beta}$-Cyclodextrin Derivative Grafted Chitosan 원문보기

Macromolecular research, v.14 no.4, 2006년, pp.443 - 448

Xiao Jian-Bo (College of Chemistry and Chemical Engineering, Central South University) , Yu Hong-Zhu (College of Chemistry and Chemical Engineering, Central South University) , Xu Ming (Research Institute for Molecular Pharmacology and Therapeutics, Central South University, Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center) , Chen Xiao-Qing (College of Chemistry and Chemical Engineering, Central South University)

Abstract ▼ AI-Helper

A novel ${\beta}$-cyclodextrin derivative (CCD-C) was synthesized with chitosan and carboxymethyl-${\beta}$-cyclodextrin. Its structure was characterized by elemental analysis, infrared spectra analysis, and X-ray diffraction analysis. The adsorption properties for guanosine 5'-monophosphate, cytidine 5'-monophosphate and uridine 5'-monophosphate were studied. Experimental results demonstrated that CCD-C had higher adsorption capability for guanosine 5'-monophosphate, and that the adsorption capacity for guanosine 5'-monophosphate was 74.20mg/g. The adsorption capacity was greatly influenced by pH, time and temperature. The introduction of chitosan enhanced the adsorption ability and adsorption selectivity of ${\beta}$-cyclodextrin for guanosine 5'-monophosphate. This novel derivative of chitosan is expected to have wide applications in the separation, concentration and analysis of nucleotides in biological samples.

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제안 방법

0 and 50 mg of CCD-C were put into an Erlenmeyer flask. After the flask was shaken at 150 rpm/ min at different temperature, namely 15, 20, 25, 30, 35, 40, 45, and 50 ℃ for 6 h, the concentrations of GMP, CMP, and UMP were determined according to section of Determination of GMP, CMP, and UMP. Figure 5 showed the effect of temperature on adsorption capacity of CCD-C.
0 and 50 mg of CCD-C were put into an Erlenmeyer flask. After the flask was shaken at 150 rpm/min at 30℃ for different time, namely 1, 2, 3, 5, 6, 7, 8, and 9 h, the concentrations of GMP, CMP, and UMP were determined according to section of Determination of GMP, CMP, and UMP. The adsorption kinetics were showed in Figure 6.
A novel CD derivative was synthesized by the reaction between chitosan and CCD. Its structure was characterized by FTIR spectra analysis, elemental analysis, X-ray diffraction analysis, and thermal analysis, which was shown in agreement with expected results. The introduction of chitosan enhanced the adsorption ability and adsorption selectivity of ^-CD for GMP.
The samples (4-15 mg) were accurately weighed in platinum pans (Netzsch) and heated at a scanning rate of 10℃/min. The characterization of the complexes in the solid state was performed by thermal analysis. The DTA profiles of CCD-C, CD, and chitosan were illustrated in Figure 3.
Infrared spectra were recorded as KBr pellets from 4000 to 450 cm-1 on a Nicolet 5DX Fourier transform infrared spectrophotometer at a resolution of 4 cm. Wide-angle X-ray diffraction (WAXD) patterns were obtained by monitoring the diffraction angle 20 from 1-70° using a Rigaku (D/MAX, 2500X) diffractometer. The diffractometer was equipped with a CuKtz (/=0.

이론/모형

1542 nm) radiation, produced under the conditions of 40 kV and 100 mA. Elemental analysis was determined with a Perkin-Elmer automatic instrument Differential thermal analysis (DTA) was carried out on a Netzsch STA 409 simultaneous thermal analyzer (Netzsch Italiana, Verona, Italy).

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