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논문 상세정보

Abstract

[ ${\beta}-Galactosidase$ ] is extensively employed in the manufacture of dairy products, including lactose-reduced milk. Here, we have isolated two gram-negative and rod-shaped coldadapted bacteria, BS 1 and HS 39. These strains were able to break down lactose at low temperatures. Although two isolates were found to grow well at $10^{\circ}C$, the BS 1 strain was unable to grow at $37^{\circ}C$. Another strain, HS-39, evidenced retarded growth at $37^{\circ}C$. The biochemical characteristics and the results of 16S rDNA sequencing identified the BS 1 isolate as Rahnella aquatilis, and showed that the HS 39 strain belonged to genus Buttiauxella. Whereas the R. aquatilis BS 1 strain generated maximal quantities of ${\beta}-galactosidase$ when incubated for 60h at $10^{\circ}C$, Buttiauxella sp. HS-39 generated ${\beta}-galactosidase$ earlier, and at slightly lower levels, than R. aquatilis BS 1. The optimum temperature for ${\beta}-galactosidase$ was $30^{\circ}C$ for R. aquatilis BS-1, and was $45^{\circ}C$ for Buttiauxella sp. HS-39, thereby indicating that R. aquatilis BS-1 was able to generate a cold-adaptive enzyme. These two cold-adapted strains, and most notably the ${\beta}-galactosidase$ from each isolate, might prove useful in some biotechnological applications.

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이 논문을 인용한 문헌 (4)

  1. Cha, In-Tae ; Lim, Hayung-Joon ; Roh, Dong-Hyun 2007. "Isolation of Pseudoalteromonas sp. HJ 47 from Deep Sea Water of East Sea and Characterization of its Extracellular Protease" 생명과학회지 = Journal of life science, 17(2): 272~278 
  2. Cha, In-Tae ; Oh, Yong-Sik ; Roh, Dong-Hyun 2007. "Isolation and Charcterization of Micrococcus sp. HJ-19 Secreting Extracellular Protease" Korean journal of microbiology = 미생물학회지, 43(3): 222~226 
  3. Oh, Kyoung-Hee ; Choi, In-Hak ; Cho, Young-Cheol 2008. "Isolation and Characterization of Ammonia-removing Bacteria from a Food-wastewater Treatment Facility" 대한환경공학회지 = Journal of Korean Society of Environmental Engineers, 30(6): 653~658 
  4. 2008. "" Journal of microbiology and biotechnology, 18(2): 189~193 

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