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Mouse Single Oral Dose Toxicity Studies of PGB-1, a Novel Polyglucosamine Polymer Produce from Enterobacter sp. BL-2

Journal of toxicology and public health : an official journal of the Korean Society of Toxicology, v.23 no.4, 2007년, pp.373 - 382  

Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ,  Son, Mi-Kyung (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ,  Jung, Young-Mi (ENZ Bio Co., Ltd.) ,  Kim, Tae-Kwon (ENZ Bio Co., Ltd.) ,  Park, Dong-Chan (ENZ Bio Co., Ltd.) ,  Lee, Hyeung-Sik (Department of Herbal Biotechnology, Daegu Haany University) ,  Kim, Pan-Soo (Department of Technical Support, Gyeonggi Bio-center) ,  Ku, Sae-Kwang (Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)

Abstract AI-Helper 아이콘AI-Helper

This study was conducted to obtain acute information of the oral dose toxicity of PGB-1, a novel polyglucosamine polymer produced from a new strain Enterobacter sp. BL-2 in male and female mice. In order to calculated 50% lethal dose ($LD_{50}$) and approximate lethal dose (LD), test mate...

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제안 방법

  • Body weight changes. Body weights were measured at the day of dosing (Day 0) immediately before treatment, 1, 2, 7, 13 and 14  days after dosing. In addition, to reduce the erratum originated from individual body weight ditferences of animals at initial dosing, body weigh gains during Day 0-Day 7, Day 7~Day 13 and Day O'Day 14 were also calculated based on measured body weight at each day.
  • Experimental animals. Each of thirty female and male ICR mice (6-wk old upon receipt, SLC, Japan) was used after acclimatization for 8 days. Five animals were allocated per a polycarbonate cage in a temperature (20'25℃) and humidity (40-45%) controlled room.
  • The objective of the present study, therefore, was to obtain the primary safety information about PGB-1, a novel polyglucosamine polymer and further clarify their safety for clinical use.

이론/모형

  • Changes of body weight were analyzed by Mann-Whitney U-Wilcoxon Rank Sum W test (MW test) compared to those of vehicle controls. LD50 was calculated by Probit method. Statistical analyses were conducted using SPSS for Windows (Release 6.
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