Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was ide...
Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri based on Gram-staining, metabolic characteristics, and 16S rDNA sequence determination, Optimum culture conditions for GABA production were determined: MRS broth containing 5% MSG, 1% NaCl, and 1% glucose, at an initial pH of 5.0, the incubation temperature at $30^{\circ}C$ for 36 h. Under these conditions, MS produced GABA at a concentration of 251 mM with a 94% GABA conversion rate. Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicantinduced cell death.
Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri based on Gram-staining, metabolic characteristics, and 16S rDNA sequence determination, Optimum culture conditions for GABA production were determined: MRS broth containing 5% MSG, 1% NaCl, and 1% glucose, at an initial pH of 5.0, the incubation temperature at $30^{\circ}C$ for 36 h. Under these conditions, MS produced GABA at a concentration of 251 mM with a 94% GABA conversion rate. Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicantinduced cell death.
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가설 설정
Amino acid standards with authentic GABA. 2. GABA produced by the isolate MS. The arrow indicates GABA (RT=13.75 min).
제안 방법
45-pm filter, and analyzed by HPLC (Hewlett Packard 1100 series). GABA analysis was performed using a HPLC equipped with a Waters symmetry C18 column (4.6x250 mm, 5 |im), a HP 1100 series binary pump, a HP 1100 series autosampler, column oven (46℃), and a HP 1100 series UV detector (254 nm). The elution solvent system was comprised of (A) 1.
To compare the GABA productivity for different carbon sources, MRS media containing 5% MSG were prepared with maltose at 1.0 or 2.0% and with arabinose, fructose, galactose, glucose, lactose at 0.5, 1.0, or 2.0%, respectively.
To determine the effect of NaCl on GABA production and cell growth, NaCl was added at 0, 1, 3, 5, or 7% (w/v) to MRS broth containing 5% MSG.
대상 데이터
The PC12 cell line was kindly provided by Dr. Hong Sung Jeon at Chosun University. The authors would like to express thanks to him.
성능/효과
buchneri MS culture had a partial or complete neuroprotective effect. These results indicated that the culture extract of Lb, buchneri MS (final concentration 10 μg/ml or 100 |ig/ml) had a partial or complete neuroprotective effect against various chemicals that induce PC12 cell death, and that it increased the proliferation of PC12 neuronal cells (Fig. 6). LAB are known to have various beneficial effects on health; i.
buchneri MS under optimal conditions for 48 h, and the highest cell growth for 36 h. Under optimal culture conditions (5% MSG 1% NaCl, and 1% glucose in MRS at an initial pH 5.0), GABA production was significantly enhanced, and reached 251 mM (GABA conversion rate: 94%) at 48 h of cultivation. Under control conditions in MRS with 5% MSG the concentration of GABA was 212 mM (a 79% conversion rate) (Fig.
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