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논문 상세정보

Abstract

Objectives : The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Rehmanniae Radix Preparat (RR). Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on H2O2-induced cytotoxicity by MTT assay and lipid peroixidation by malondialdehyde (MDA) formation, respectively. Results : The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to 43.1%. The extract at concentrations of 100 ${\mu}g/ml$ showed peak level of 136.5% in growth of GC-1 cell. The hydrogen peroxide-induced cytotoxicity was blocked by the extract at concentrations of 10, 50 and 100 ${\mu}g/ml$. The extract (50 and 100 ${\mu}g/ml$) also displayed a dose-dependent reduction of MDA formation on hydrogen peroxide-induced lipid peroxidation. Conclusions : In conclusion, the extract of Rehmanniae Radix Preparat has strong antioxidant activity.

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이 논문을 인용한 문헌 (4)

  1. Kim, Hyung-Woo ; Kim, Kyung-Yoon ; Kim, Gye-Yep ; Kim, Chae-Hyun ; Jeong, Jong-Gil ; Choi, Chan-Hun ; Hwang, Gui-Seong ; Lee, Sang-Yeong ; Jeong, Hyun-Woo 2009. "Protective Effects of Samul-tang on Cell Death Inducded by Oxidative Stress in C6 Glial Cell" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 23(5): 969~973 
  2. Lee, Sang-Yeong ; Kim, Hyung-Woo ; Kim, Gye-Yep ; Choi, Chan-Hun ; Yun, Yeo-Chung ; Jeong, Hyun-Woo 2009. "Protective Effects and Anti-oxidative Effects of Sipjeon-Daebo-Tang and Gami-Sipjeon-Daebo-Tang in C6 Glioma Cell" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 23(6): 1292~1298 
  3. Han, Jong-Hyun ; Park, Bo-Ra 2011. "Study on The Componential Changes of Rehmannia Radix Preparata by Steaming and Hot-air Drying" 동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, 25(5): 823~829 
  4. Bae, Gi-Sang ; Kim, Hyun Sik ; Park, Kyoung-Chel ; Choi, Sun-Bok ; Jo, Il-Joo ; Lee, Chang-Hyuk ; Seo, Sang-Wan ; Kim, Jong-Jin ; Shin, Yong-Kook ; Kim, Min Sun ; Park, Kyu Hwan ; Song, Ho-Joon ; Park, Sung-Joo 2012. "Effects of OMC-2010 constituents on cytokine productions in mouse spleen cells" 大韓本草學會誌 = The Korea journal of herbology, 27(6): 49~54 

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