서로 다른 지역에서 채집된 Sulfonylurea계 제초제 저항성 물달개비의 제초제 반응 차이 Differential herbicide response of sulfonylurea-resistant Monochoria vagnalis accessions to sulfonylurea herbicides원문보기
4개의 서로 지역 계통별 sulfonylurea(SU)계 제초제 저항성 물달개비의 저항성 정도 차이를 구명하기 위하여 SU계 제초제들에 대한 식물체 및 acetolactate synthase(ALS) 반응 차이를 감수성 계통과 비교 분석하였다. 나주, 논산, 김제 지역에서 채집된 저항성 물달개비들은 SU계 제초제들의 기준량에서 거의 영향을 받지 않았다. 사용된 SU계 제초제들의 기준량에 대한 나주, 논산, 김제 지역계통의 건물 중 50% 억제 제초제 농도인 $GR_{50}$은 감수성계통(청도) 보다 각각 $8{\sim}33$배, $8{\sim}30$배, $7{\sim}32$배 높게 나타났다. 그러나 김해 채집계통의 $GR_{50}$은 감수성 채집계통에 비해 $4{\sim}13$배 높게 나타나 중간정도의 저항성을 보였다. 나주, 논산, 김제에 대한 ALS 50% 억제 제초제 농도인 $I_{50}$은 감수성 채집계통에 비해 각각 $25{\sim}66$배, $9{\sim}26$배, $10{\sim}24$배 높게 나타났다. 그러나 김해 채집계통의 91%은 감수성 채집계통에 비해 $4{\sim}9$배 높게 나타났다.
4개의 서로 지역 계통별 sulfonylurea(SU)계 제초제 저항성 물달개비의 저항성 정도 차이를 구명하기 위하여 SU계 제초제들에 대한 식물체 및 acetolactate synthase(ALS) 반응 차이를 감수성 계통과 비교 분석하였다. 나주, 논산, 김제 지역에서 채집된 저항성 물달개비들은 SU계 제초제들의 기준량에서 거의 영향을 받지 않았다. 사용된 SU계 제초제들의 기준량에 대한 나주, 논산, 김제 지역계통의 건물 중 50% 억제 제초제 농도인 $GR_{50}$은 감수성계통(청도) 보다 각각 $8{\sim}33$배, $8{\sim}30$배, $7{\sim}32$배 높게 나타났다. 그러나 김해 채집계통의 $GR_{50}$은 감수성 채집계통에 비해 $4{\sim}13$배 높게 나타나 중간정도의 저항성을 보였다. 나주, 논산, 김제에 대한 ALS 50% 억제 제초제 농도인 $I_{50}$은 감수성 채집계통에 비해 각각 $25{\sim}66$배, $9{\sim}26$배, $10{\sim}24$배 높게 나타났다. 그러나 김해 채집계통의 91%은 감수성 채집계통에 비해 $4{\sim}9$배 높게 나타났다.
Four sulfonylurea(SU)-resistant Monochoria vaginalis(M. vaginalis) accessions were tested for levels of resistance to four SU herbicides which have been widely using in paddy fields of Korea, based on whole plant response and sensitivity of the target enzyme, acetolactate synthase(ALS). The resistan...
Four sulfonylurea(SU)-resistant Monochoria vaginalis(M. vaginalis) accessions were tested for levels of resistance to four SU herbicides which have been widely using in paddy fields of Korea, based on whole plant response and sensitivity of the target enzyme, acetolactate synthase(ALS). The resistant Naju, Nonsan and Gimje accessions were not affected to the survival by treatment with recommended dose of all SU herbicides tested. The $GR_{50}$ values for the Naju, Nonsan and Gimje accessions were 8- to 33-fold, 8- to 30-fold and 7- to 32-fold higher to recommended doses of all SU herbicides tested than the susceptible Cheongdo accession, respectively. However, the $GR_{50}$ values for Kimhae accession displayed an intermediate response and was only 4-to 13-fold more resistant than the susceptible accession. The ALS $I_{50}$ values for the Naju, Nonsan and Gimje accessions were 25- to 66-fold, 9- to 26-fold and 10- to 24-fold higher to recommended doses of all SU herbicides tested than the susceptible Cheongdo accession, respectively. However, the $I_{50}$ value for Kimhae accession was 4- to 9-fold more resistant than the susceptible accession, as determined by $I_{50}$ values of ALS.
Four sulfonylurea(SU)-resistant Monochoria vaginalis(M. vaginalis) accessions were tested for levels of resistance to four SU herbicides which have been widely using in paddy fields of Korea, based on whole plant response and sensitivity of the target enzyme, acetolactate synthase(ALS). The resistant Naju, Nonsan and Gimje accessions were not affected to the survival by treatment with recommended dose of all SU herbicides tested. The $GR_{50}$ values for the Naju, Nonsan and Gimje accessions were 8- to 33-fold, 8- to 30-fold and 7- to 32-fold higher to recommended doses of all SU herbicides tested than the susceptible Cheongdo accession, respectively. However, the $GR_{50}$ values for Kimhae accession displayed an intermediate response and was only 4-to 13-fold more resistant than the susceptible accession. The ALS $I_{50}$ values for the Naju, Nonsan and Gimje accessions were 25- to 66-fold, 9- to 26-fold and 10- to 24-fold higher to recommended doses of all SU herbicides tested than the susceptible Cheongdo accession, respectively. However, the $I_{50}$ value for Kimhae accession was 4- to 9-fold more resistant than the susceptible accession, as determined by $I_{50}$ values of ALS.
Data were subjected to analysis of variance and pooled ov^ both experimental repetitions since there were no between- experiment interacticms. Nfeans of the five accesscMK within herbicide rate combinations were analyzed separately using the LSD test (P < 0.05). Data are reported as percent reduction in biomass con中ared to the untreated controls of each accession
The intennediate respcmse of the Kimhae accession is also of interest, since it may reflect a only low levels of SU resistance. Resistance level differences among these accessions were not due to altered amounts of the ALS enzyme, since ALS specific activity was similar in all cases to the response of whole plant by four SU herbicides tested. We do not believe that alt^ed uptate, translocation, or metabolim contributed to the differences in v4iole plant resistance measured here, since these processes were not affected in SU-resistant M.
The purposes of our research were to conduct a con^rehensive analysis of the sensitivity of four resistant M. vaginalis accessions to several SU herbicides at the 価ole plant and target enzyme levels, and investigate to management of SU-resistant M. vaginalis to several alternative herbicides that do not inhibit ALS activity.
성능/효과
is shown in Table 1. Fresh weights(GR5o) of the Naju, Nonsan and Gimje accessions were not affected by at the normal field use rate of SU herbicides applied, and reduced by about from 30% to 40% to untreated plant fresh weight even at 10X the normal field use rate of all herbicides. In contrast, Cheon흥 do accession was much mere sensitive to the other resistant accessions: fresh weight was reduced by more than 90% by these treatments.
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