[논문]Populations of Fungi and Bacteria Associated with Samples of Stored Rice in Korea

Oh, Ji-Yeon; Jee, Sam-Nyu; Nam, Young-Woo; Lee, Ho-Joung; Ryoo, Mun-Il; Kim, Ki-Deok

doi:10.4489/myco.2007.35.1.036

Populations of Fungi and Bacteria Associated with Samples of Stored Rice in Korea 원문보기

Mycobiology, v.35 no.1, 2007년, pp.36 - 38

Oh, Ji-Yeon (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) , Jee, Sam-Nyu (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) , Nam, Young-Woo (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) , Lee, Ho-Joung (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) , Ryoo, Mun-Il (Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University) , Kim, Ki-Deok (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University)

Abstract ▼ AI-Helper

Stored rice was collected from rice processing complexes of National Agricultural Cooperative Federation of 11 regions in Korea to evaluate the occurrence of fungi and bacteria and to identify the predominant fungi and bacteria to the genus levels. Most rice samples generally produced the higher levels of fungi and bacteria than white rice. The occurrence of fungi and bacteria varied in various locations of Korea. Among fungi observed, Aspergillus spp. and Penicillium spp. were dominant in the samples and Aspergillus spp. were observed more frequently than Penicillium spp. Predominant bacteria from rice and white rice samples tentatively belonged to the Genus Bacillus, Pectobacterium, Pantoea, and Microbacterium according to BIOLOG and FAME analyses. The results of this study showed that rice in Korea was contaminated in a relatively high level by two dominant storage fungi such as Aspergillus spp. and Penicillium spp. In addition, occurrence of mycotoxins in rice by the fungi could be possible and thus it is necessary to control the storage fungi.

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문제 정의

For the control of storage fungi, the basic information on the population of natu rally occurring fungi and bacteria is essential. Therefore, the objectives of this study were to evaluate the occur rence of fungi and bacteria in rice and white rice stored in rice processing complexes (RPC) of National Agricul tural Cooperative Federation and to identify the predomi nant fungi and bacteria to the genus levels.

제안 방법

01A (BIOLOQ Hayward, USA) at 24 hr. Along with BIOLOG analysis, the analysis of fatty acid methyl-esters (FAME) for the representative bacte rial strains was conducted for identification to the genus level by gas chromatography (GC) using the MIDI sys tem (Microbial Identification System, Inc., Newark, USA). Bacterial cells were harvested from colonies grown on TSA and then fatty acids were isolated from the cellu lar lipids by saponification.
The electronic signal of the GC was passed to the computer, which com pared the fatty acid methyl ester composition of the tested bacteria to those of known bacteria in a stored database library to select the most similar species with the tested bacteria. The experiments were conducted with three rep lications and the data were analyzed with the Statistical Analysis System (SAS Institute, Cary, USA).

후속연구

Based on the basic information on pop ulation and diversity of bacteria, searching biologically effective bacteria as biocontrol agents from rice could be needed to reduce the fungal contamination. Further studies on current situation of mycotoxins occurring in rice in Korea by the fiingus should be needed.

참고문헌 (11)

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