Chang, Ho-Won
(Biological Resource Center, KRIBB)
,
Bae, Jin-Woo
(Biological Resource Center, KRIBB)
,
Nam, Young-Do
(Biological Resource Center, KRIBB)
,
Kwon, Hyuk-Yong
(Biological Resource Center, KRIBB)
,
Park, Ja-Ryeong
(Biological Resource Center, KRIBB)
,
Shin, Kee-Sun
(Biological Resource Center, KRIBB)
,
Kim, Kyoung-Ho
(Biological Resource Center, KRIBB)
,
Quan, Zhe-Xue
(Biological Resource Center, KRIBB)
,
Rhee, Sung-Keun
(Department of Microbiology, Chungbuk National University)
,
An, Kwang-Guk
(Department of Biology, Chungnam National University)
,
Park, Yong-Ha
(Biological Resource Center, KRIBB)
Strain $CH7^T$, a pale yellow-pigmented bacterium and new isolate from deep subsurface water of the South Coast of Korea, was subjected to a polyphasic taxonomic study. $CH7^T$ grew between 5 and $37^{\circ}C$, pH 5.3-10.5, and tolerated up to 13% NaCl. A phylogeneti...
Strain $CH7^T$, a pale yellow-pigmented bacterium and new isolate from deep subsurface water of the South Coast of Korea, was subjected to a polyphasic taxonomic study. $CH7^T$ grew between 5 and $37^{\circ}C$, pH 5.3-10.5, and tolerated up to 13% NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $CH7^T$ was associated with the genus Arthrobacter and phylogenetically closely related to the type strains Arthrobacter tumbae (99.4%) and Arthrobacter parietis (99.1%). However, DNA-DNA hybridization experiments revealed 2.1% and 12% between strain $CH7^T$ and Arthrobacter tumbae and Arthrobacter parietis, respectively. Thus, the phenotypic and phylogenetic differences suggested that $CH7^T$ should be placed in the genus Arthrobacter as a novel species, for which the name Arthrobacter subterraneus sp. novo is proposed. In addition, the type strain for the new species is $CH7^T$ ($=KCTC\;9997^T=DSM\;17585^T$).
Strain $CH7^T$, a pale yellow-pigmented bacterium and new isolate from deep subsurface water of the South Coast of Korea, was subjected to a polyphasic taxonomic study. $CH7^T$ grew between 5 and $37^{\circ}C$, pH 5.3-10.5, and tolerated up to 13% NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $CH7^T$ was associated with the genus Arthrobacter and phylogenetically closely related to the type strains Arthrobacter tumbae (99.4%) and Arthrobacter parietis (99.1%). However, DNA-DNA hybridization experiments revealed 2.1% and 12% between strain $CH7^T$ and Arthrobacter tumbae and Arthrobacter parietis, respectively. Thus, the phenotypic and phylogenetic differences suggested that $CH7^T$ should be placed in the genus Arthrobacter as a novel species, for which the name Arthrobacter subterraneus sp. novo is proposed. In addition, the type strain for the new species is $CH7^T$ ($=KCTC\;9997^T=DSM\;17585^T$).
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대상 데이터
Gram-positive, and pale-yellow pigmented bacterial strain CH7T was isolated using a dilution-plating technique from deep subsurface water collected from the South Coast of Korea. The collection area was located in the center of the Pohang basin from the Southeastern Coast of the Korean peninsula, and was the focus of drilling for deep hydrothermal systems to utilize subterranean heat energy. The basin has a basement consisting of Cretaceous sedimentary rocks, including tuflfs and granites, which are covered with Miocene Yeonil formations, semiconsolidated clastic sedimentary rocks with marine and terrestrial origins.
이론/모형
1 software. The distance matrices were determined following the assumptions described by Kimura [13], and these matrices used to elaborate dendrograms using the neighbor-joining method [24]. A bootstrap analysis to investigate the stability of the trees was then performed by obtaining a consensus tree based on 1, 000 randomly generated trees.
The tree was generated using the neighbor-joining method. The numbers at the nodes indicate the bootstrap values (1, 000 replications).
성능/효과
1). CH7t exhibited the highest 16S rDNA similarity to Arthrobacter parietis1 (99.1%), Arthrobacter tumbae1 (99.4%), Arthrobacter tecti1 (98.5%), and Arthrobacter agilis1 (98.1%). DNA-DNA homology studies were then performed to determine the genomic relationship between CH7t and type strains from the closest relatives as regards 16S rDNA similarity.
The Gram reaction was determined using a Gram Stain kit (Difco) according to the manufacturer^ instructions. For reference strains, the most closely related strains, based on 16S rDNA similarity, Arthrobacter tumbae DSM 164061, Arthrobacter parietis DSM 1 6404t, Arthrobacter tecti DSM 16407T, and Arthrobacter agilis DSM 20550T, were obtained from DSMZ, Germany, and grown under the same conditions. For morphological and physiological characterization, strain CH7t was generally cultivated in a nutrient broth (NB) and incubated by shaking at 25°C.
pigmenti. Furthermore, the predominant fatty acids for CH가 were anteiso-C15:0 (35.4%) and anteiso-C17:1o)9c (15%), whereas those for A. flavus were anteiso-C15:0 (52%) and iso-C16;0 (17%), those for A parietis were anteiso- C15;o (51%) and iso-C15;0 (29%), those for A. tumbae were anteiso-C15.0 (57%) and iso-C15.0 (17%), and those for 4 tecti were anteiso-C15.0 (44%) and iso-C15:0 (37%).
roseus) [8, 9, 11, 12, 14, 16, 17, 21, 22, 27, 32], The prominent characteristic that differentiated strain CH7T from other related species was the production of a pale-yellow pigment. Moreover, while exhibiting certain phenotypically recognizable traits, strain CH7T displayed a strong valine arylamidase activity in contrast to the type strains of phylogenetically closely related species, such as A. tectiy A. tumbae^ A. castelli, A. monument^ A. parietis^ and A. pigmenti. Furthermore, the predominant fatty acids for CH가 were anteiso-C15:0 (35.
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