[논문]Halomonas subglaciescola DH-1의 생장에 미치는 염화나트륨의 영향

나병관; 유영선; 박두현

Halomonas subglaciescola DH-1의 생장에 미치는 염화나트륨의 영향
Effect of NaCl on Halomonas subglaciescola DH-1 Incapable of Growing at Non-Salinity 원문보기

한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, v.35 no.4, 2007년, pp.298 - 303

나병관 (서경대학교 생물공학과) , 유영선 (서경대학교 응용화학과) , 박두현 (서경대학교 생물공학과)

초록
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호염성세균 H. subglaciescola DH-1은 염화나트륨이 없거나 0.8 M 이하로 존재하는 환경에서 생장하지 못한다. 이 호혐성세균은 2.0 M의 염화나트륨이 존재하는 조건에서는 최적온도($30^{\circ}C$)보다 높은 $40^{\circ}C$에서 생장이 가능하였으나, 0.8 M의 염화나트륨이 존재하는 조건에서는 생장이 크게 저하되었다. 세포추출물을 염화나트륨이 존재하는 조건에서 $50^{\circ}C$로 1시간 동안 열처리하였을 때 세포내 효소의 활성이 유지되었으나, 염화나트륨이 없는 조건에서 열처리하였을 때 효소의 활성은 유지되지 않았다. 반면, 대장균의 세포추출물의 효소활성은 1.0 M이상의 염화나트륨이 존재할 때 온도 또는 pH와 관계없이 측정되지 않았다. H. subglaciescola DH-1은 pH $7{\sim}10$의 범위에서 생장하였고, 생장을 위한 최적 pH는 8이었다. 이러한 생리적인 특성으로부터 염화나트륨은 H. subglaciescola DH-1의 물질대사를 위한 필수적인 무기영양소라는 사실을 유추할 수 있다.

Abstract ▼ AI-Helper

A halophilic bacterium, H. subglaciescola DH-1, grew at 2.0 M salinity, but did not grow at 0.8 M salinity when cultivated at higher temperature ($40^{\circ}C$) than optimum ($30^{\circ}C$). When the cell extract of strain DH-1 was heated at $50^{\circ}C$ for 60 min in the absence of NaCl, isocitrate dehydrogenase and malate dehydrogenase lost their activities, but when it was heated in the presence of 2.0 M NaCl, the activity was maintained. Meanwhile, the cell extract of E. coli did not catalyze the reduction of $NAD^+$ to NADH coupled with the oxidation of isocitrate and malate at higher salinities than 1.0 M. The pH range for DH-1 was 7 to 10, and that for E. coli was 5 to 9. DH-1 was not grown in conditions with sodium salts other than NaCl.

주제어

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가설 설정

5 M [6]. H. variabilis was dependent on the compatible solutes to protect themselves against high salinity [11, 12]. A mutant of H.

제안 방법

In this study, we tested the effect of NaCl on viability and intracellular enzymes in unfavorable conditions, and compared the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pattern of the cell-free extract of DH-1 grown at different salinities to verify the influence of NaCl on the physiology of H. subglaciescola DH-1.

성능/효과

The iCDH and MDH may be not representative of all intracellular enzymes, but are essential enzymes for energy metabolism, which are only found in the TCA cycle. The most likely explanation of these results is that NaCl may be absolutely required for some intracellular enzymes of DH-1, and a proper concentration of NaCl may still exist in the cytoplasm. Some data reported by other researchers show results that are very similar to ours.

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