목 적 : 지금까지 코로나바이러스는 대부분 상기도 감염을 일으키며 임상적으로 큰 의미가 없는 것으로 여겨져 왔다. 하지만 최근에 발견된 코로나 바이러스인 hCoV-NL63와 hCoV-HKU1는 하부 호흡기 질환과의 연관성이 있는 것으로 보고되면서 임상적 의의에 대한 연구가 필요한 실정이다. 이에 저자들은 폐렴으로 입원한 소아 환자에서 최근에 발견된 hCoV-NL63과 hCoV-HKU1을 포함한 코로나바이러스 감염의 유병률을 알아보기 위하여 본 연구를 시행하였다. 방 법 : 2006년 3월부터 2007년 1월까지 폐렴으로 상계백병원에 입원한 소아에게서 비인두 흡인물을 수집하였다. Multiplex RT-PCR 방법을 이용하여 RSV, influenza A, influenza B, parainfluenzavirus 및 adenovirus 감염을 진단하였으며, F 유전자 시발체를 이용한 nested RT-PCR에 의해 hMPV 감염을 진단하였다. 코로나바이러스 감염을 진단하기 위해 hCoVNL63, hCoV-OC43, hCoV-229E 및 hCoV-HKU1에 각각 특이적 시발체를 이용하여 nested RT-PCR을 시행하였다. 결 과 : 총 217명의 입원한 15세 이하의 폐렴 환아에게서 수집한 비인두 흡인물에서 multiplex PCR 방법에 의해 RSV 양성은 32명, hMPV는 18명, parainfluenza virus는 10명, influenza virus A는 2명, adenovirus는 6명에서 양성이었다. RT-PCR에 의해 hMPV 양성은 18명에서 확인되었다. 코로나바이러스는 RT-PCR 방법에 의해 8명 (3.7%)에서 양성이었으며, hCoV-229E 1명, hCoV-NL63 3명, 그리고 hCoV-OC43가 4명에서 검출되었다. 하지만 hCoV- HKU1는 연구 대상군에서 검출되지 않았다. 결 론 : 아직 추가 연구가 필요하지만 최근에 발견된 hCoV-HKU1와 hCoV-NL63은 폐렴으로 입원한 국내 소아에서 임상적인 중요성이 적을 것으로 생각된다.
목 적 : 지금까지 코로나바이러스는 대부분 상기도 감염을 일으키며 임상적으로 큰 의미가 없는 것으로 여겨져 왔다. 하지만 최근에 발견된 코로나 바이러스인 hCoV-NL63와 hCoV-HKU1는 하부 호흡기 질환과의 연관성이 있는 것으로 보고되면서 임상적 의의에 대한 연구가 필요한 실정이다. 이에 저자들은 폐렴으로 입원한 소아 환자에서 최근에 발견된 hCoV-NL63과 hCoV-HKU1을 포함한 코로나바이러스 감염의 유병률을 알아보기 위하여 본 연구를 시행하였다. 방 법 : 2006년 3월부터 2007년 1월까지 폐렴으로 상계백병원에 입원한 소아에게서 비인두 흡인물을 수집하였다. Multiplex RT-PCR 방법을 이용하여 RSV, influenza A, influenza B, parainfluenzavirus 및 adenovirus 감염을 진단하였으며, F 유전자 시발체를 이용한 nested RT-PCR에 의해 hMPV 감염을 진단하였다. 코로나바이러스 감염을 진단하기 위해 hCoVNL63, hCoV-OC43, hCoV-229E 및 hCoV-HKU1에 각각 특이적 시발체를 이용하여 nested RT-PCR을 시행하였다. 결 과 : 총 217명의 입원한 15세 이하의 폐렴 환아에게서 수집한 비인두 흡인물에서 multiplex PCR 방법에 의해 RSV 양성은 32명, hMPV는 18명, parainfluenza virus는 10명, influenza virus A는 2명, adenovirus는 6명에서 양성이었다. RT-PCR에 의해 hMPV 양성은 18명에서 확인되었다. 코로나바이러스는 RT-PCR 방법에 의해 8명 (3.7%)에서 양성이었으며, hCoV-229E 1명, hCoV-NL63 3명, 그리고 hCoV-OC43가 4명에서 검출되었다. 하지만 hCoV- HKU1는 연구 대상군에서 검출되지 않았다. 결 론 : 아직 추가 연구가 필요하지만 최근에 발견된 hCoV-HKU1와 hCoV-NL63은 폐렴으로 입원한 국내 소아에서 임상적인 중요성이 적을 것으로 생각된다.
Purpose : Human coronanviruses (hCovs) including hCoV-229E and hCoV-OC43 have been known as etiologic agents of the common colds and were regarded as clinically insignificant agents. However, recent identification of hCoV-NL63 and hCoV-HKU1 in children with lower respiratory tract infections has evo...
Purpose : Human coronanviruses (hCovs) including hCoV-229E and hCoV-OC43 have been known as etiologic agents of the common colds and were regarded as clinically insignificant agents. However, recent identification of hCoV-NL63 and hCoV-HKU1 in children with lower respiratory tract infections has evoked the clinical concerns about their prevalence and the clinical significance of these hCoVs in children. This study was performed to investigate the prevalence of hCoVs in children with community-acquired pneumonia. Methods : From March 2006 to January 2007, nasopharyngeal specimens collected from children hospitalized with pneumonia, were tested for the presence of common respiratory viruses (respiratory syncytial virus, influenza A, influenza B, parainfluenza viruses, and adenovirus) using multiplex reverse transcriptase polymerase chain reaction (RT-PCR). Human metapneumovirus (hMPV) infection was excluded by nested RT-PCR using primers for the F-gene. To detect the different strains of hCoVs, nested RT-PCR assays specific for hCoVNL63, hCoV-OC43, hCoV-229E, and hCoV-HKU1 were performed. Results : Out of the 217 nasopharyngeal aspirate from children aged under 15 years, respiratory syncytial virus (RSV) was detected in 32 patients, hMPV in 18, human parainfluenza virus in 10, influenza virus A in 2, and adenovirus in 6. HCoVs were detected by RT-PCR in 8 (3.7%) of the 217 patients, hCoV-229E in 1, hCoV-NL63 in 3, and hCoVOC43 in 4 patients. HCoV-HKU1 was not detected in this study population. Conclusion : Recently identified hCoV-NL63 and hCoV-HKU1 seemed to have a little clinical significance in Korean children with severe or hospitalized community-acquired pneumonia.
Purpose : Human coronanviruses (hCovs) including hCoV-229E and hCoV-OC43 have been known as etiologic agents of the common colds and were regarded as clinically insignificant agents. However, recent identification of hCoV-NL63 and hCoV-HKU1 in children with lower respiratory tract infections has evoked the clinical concerns about their prevalence and the clinical significance of these hCoVs in children. This study was performed to investigate the prevalence of hCoVs in children with community-acquired pneumonia. Methods : From March 2006 to January 2007, nasopharyngeal specimens collected from children hospitalized with pneumonia, were tested for the presence of common respiratory viruses (respiratory syncytial virus, influenza A, influenza B, parainfluenza viruses, and adenovirus) using multiplex reverse transcriptase polymerase chain reaction (RT-PCR). Human metapneumovirus (hMPV) infection was excluded by nested RT-PCR using primers for the F-gene. To detect the different strains of hCoVs, nested RT-PCR assays specific for hCoVNL63, hCoV-OC43, hCoV-229E, and hCoV-HKU1 were performed. Results : Out of the 217 nasopharyngeal aspirate from children aged under 15 years, respiratory syncytial virus (RSV) was detected in 32 patients, hMPV in 18, human parainfluenza virus in 10, influenza virus A in 2, and adenovirus in 6. HCoVs were detected by RT-PCR in 8 (3.7%) of the 217 patients, hCoV-229E in 1, hCoV-NL63 in 3, and hCoVOC43 in 4 patients. HCoV-HKU1 was not detected in this study population. Conclusion : Recently identified hCoV-NL63 and hCoV-HKU1 seemed to have a little clinical significance in Korean children with severe or hospitalized community-acquired pneumonia.
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문제 정의
However, the epidemiology of hCoVHKU1 in Korean children is not known. The purpose of this study was to investigate the epidemiology of hCoV infections in children hospitalized for community-acquired pneumonia.
제안 방법
PCR for coronaviruses was performed using four different specific primers to amplify hCoV-HKU1, hCoV-NL63, hCoV-OC43, and hCoV-229E, respectively, as described previously11, 12, 19, 21) (Table 1). To detect HCoV-NL63, previously described nested PCR assays for 1a and 1b gene were performed.
Each primer mixture contained cDNA, PCR buffer, 200 uM concentration of each deoxynucleoside triphosphate, and 1 U Taq polymerase. PCR was done using the following reaction conditions: initial denaturation at 94℃ for 5 minute; 30 cycles of 94℃ for 30 seconds, 55℃ for 30 seconds, and 72℃ for 1 minute; and final extension at 72℃ for 10 minutes. The PCR products were separated by electrophoresis in 2% agarose gel, visualized with ethidium bromide and were sequenced with an ABI prism 3700 DNA analyzer (Applied Biosystem, Foster City, CA) to confirm the presence of hCoVs.
대상 데이터
A total of 217 children aged less than 15 years were enrolled (120 males; mean age, 23.9 months). The age distribution of the study population was: 27.
성능/효과
In conclusion, recently identified hCoV-NL63 and hCoV-HKU1 seemed to have a little clinical significance in Korean children with severe or hospitalized community-acquired pneumonia. However, further studies are needed to clarify the epidemiological and clinical features of hCoVs including hCoV-HKU1 in children with lower respiratory tract infections.
후속연구
In conclusion, recently identified hCoV-NL63 and hCoV-HKU1 seemed to have a little clinical significance in Korean children with severe or hospitalized community-acquired pneumonia. However, further studies are needed to clarify the epidemiological and clinical features of hCoVs including hCoV-HKU1 in children with lower respiratory tract infections.
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