본 연구는 자생 새우난초의 형태적 특성을 조사하고, RAPD법을 이용하여 유전적 다양성을 분석하고자 수행되었다. 자생지에서 화색을 포함한 19가지의 형태적 특성 분류 기준에 따라 새우난초, 금새우난초, 변이종을 각각 3종류씩 9종류를 선발하였다. 잎의 길이와 넓이, 주판(dorsal sepal), 부판(lateral sepal), 꽃잎(petal), 중심 설(central lip),측면 설(lateral lip)은 길이와 넓이에 있어서 새우난초가 가장 짧고 좁았으며, 금새우난초가 가장 길고 넓었다. 화경(flower stalk)의 길이는 새우난초가 가장 짧았고, 금새우난초가 가장 길었으며, 변이종은 위의 각기관의 길이와 넓이에 있어서 새우난초와 금새우난초의 중간정도였다 그러나 거(spur)의 길이는 새우난초가 가장 길었으며, 변이종, 금새우난초의 순이었다. 자방(ovary)의 길이는 새우난초가 가장 짧았고, 금새우난초와 변이종은 비슷하였다. 새우난초의 화색은 CIE Lab 값이 40에서 50사이의 갈색계통이었으며, 금새우난초는 CIE Lab값이 110에서 130사이의 밝은 황색계통이었다. 변이종은 CIE tab 값이 50에서 70사이의 다양한 색을 나타내었다. 유전적 유연관계를 조사하기 위하여 multiple band의 양상을 분석한 결과, 총 305개의 band 중 154개의 polymorphic band를 선발하였다. 이들의 유연관계는 새우난초와 금새우난초가 가장 멀어 새우난초와 금새우난초는 다른 종에 속해있음을 알 수 있었고, 변이종은 유전적으로 새우난초와 금새우난초의 중간에 위치하고 있음을 알 수 있었다.
본 연구는 자생 새우난초의 형태적 특성을 조사하고, RAPD법을 이용하여 유전적 다양성을 분석하고자 수행되었다. 자생지에서 화색을 포함한 19가지의 형태적 특성 분류 기준에 따라 새우난초, 금새우난초, 변이종을 각각 3종류씩 9종류를 선발하였다. 잎의 길이와 넓이, 주판(dorsal sepal), 부판(lateral sepal), 꽃잎(petal), 중심 설(central lip),측면 설(lateral lip)은 길이와 넓이에 있어서 새우난초가 가장 짧고 좁았으며, 금새우난초가 가장 길고 넓었다. 화경(flower stalk)의 길이는 새우난초가 가장 짧았고, 금새우난초가 가장 길었으며, 변이종은 위의 각기관의 길이와 넓이에 있어서 새우난초와 금새우난초의 중간정도였다 그러나 거(spur)의 길이는 새우난초가 가장 길었으며, 변이종, 금새우난초의 순이었다. 자방(ovary)의 길이는 새우난초가 가장 짧았고, 금새우난초와 변이종은 비슷하였다. 새우난초의 화색은 CIE Lab 값이 40에서 50사이의 갈색계통이었으며, 금새우난초는 CIE Lab값이 110에서 130사이의 밝은 황색계통이었다. 변이종은 CIE tab 값이 50에서 70사이의 다양한 색을 나타내었다. 유전적 유연관계를 조사하기 위하여 multiple band의 양상을 분석한 결과, 총 305개의 band 중 154개의 polymorphic band를 선발하였다. 이들의 유연관계는 새우난초와 금새우난초가 가장 멀어 새우난초와 금새우난초는 다른 종에 속해있음을 알 수 있었고, 변이종은 유전적으로 새우난초와 금새우난초의 중간에 위치하고 있음을 알 수 있었다.
This study was conducted to research the morphological characteristics and analyze the genetic diversity by using RAPD in Calanthe species native to Korea. Nine samples were selected by flower color and 19 morphological characteristics. In the length and width of leaf, dorsal sepal, the lateral sepa...
This study was conducted to research the morphological characteristics and analyze the genetic diversity by using RAPD in Calanthe species native to Korea. Nine samples were selected by flower color and 19 morphological characteristics. In the length and width of leaf, dorsal sepal, the lateral sepal, the petal, the central lip, and the lateral lip, C. discolor was the shortest and narrowest, respectively, but C. sieboldii was the longest and the widest, respectively. The flower stalk length was the shortest in C. discolor, and the longest in C. sieboldii. Three variants were the intermediate between C. discolor and C. sieboldii in the above morphological characteristics, but spur length was the longest in C. discolor, the shortest in C. sieboldii, and intermediate in the variants. The ovary length in C. discolor was shortest and C. sieboldii and variants were similar with each other. The flower color of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. sieboldii was yellowish, the value of CIE Lab was between 110 and 130. And variants had various colors between 50 to 70 in the value of CIE Lab. By analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of genetic similarity of Calanther species using RAPD showed that C. discolor and C. sieboldii are more distant from each other than variants, and these results demonstrated that genetic position of variants located between C. discolor and C. sieboldii.
This study was conducted to research the morphological characteristics and analyze the genetic diversity by using RAPD in Calanthe species native to Korea. Nine samples were selected by flower color and 19 morphological characteristics. In the length and width of leaf, dorsal sepal, the lateral sepal, the petal, the central lip, and the lateral lip, C. discolor was the shortest and narrowest, respectively, but C. sieboldii was the longest and the widest, respectively. The flower stalk length was the shortest in C. discolor, and the longest in C. sieboldii. Three variants were the intermediate between C. discolor and C. sieboldii in the above morphological characteristics, but spur length was the longest in C. discolor, the shortest in C. sieboldii, and intermediate in the variants. The ovary length in C. discolor was shortest and C. sieboldii and variants were similar with each other. The flower color of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. sieboldii was yellowish, the value of CIE Lab was between 110 and 130. And variants had various colors between 50 to 70 in the value of CIE Lab. By analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of genetic similarity of Calanther species using RAPD showed that C. discolor and C. sieboldii are more distant from each other than variants, and these results demonstrated that genetic position of variants located between C. discolor and C. sieboldii.
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제안 방법
To analyze the DNA of individuals, we selected one hundred 10-mer random arbitrary primers of OPA, OPB, OPC, OPD, and OPE-s아 (Operon Technologies, California, USA). DNA amplification reactions were performed in 0.
대상 데이터
The number of amplified bands for each ten-mer primer varied from 2 to 8, with an average of around 4 bands per primer accounting for a total of 305 bands from 100 primers. After analyzing the multiple band patterns of the PCR products, 154 bands of 305 bands were selected as polymorphic RAPD markers. The size of the amplified products ranged from 0.
Nine plant materials were selected randomly at habitats in Jeju, Korea. Plant materials were consisted of 3 plants of C.
Jeju, Korea. Plant materials were consisted of 3 plants of C. discolor and 3 plants of C, sieboldii, and the 3 plants of variants which thought to be crossed between C. discolor and C. sieboldii (Fig. 1). Morphological characteristics survey was conducted from middle April after flowering and survey methods were as follows.
이론/모형
0 to 2,000 base pair. Phylogenetic similarity coefficients of each strain were quantified using the Ntsys ver. 2.11 computer program [25], A cluster analysis was done using the unweighted pair group method with arithmetic (UPGMA).
sieboldii, and 3 of variants were selected a same habitat in Jeju; Korea.The DNA of each plant was extracted from young leaves using cetyltrimehylammonium bromide (CTAB) method.
성능/효과
bicolor was intermediate between the two species. The length and width of dorsal and lateral sepal, petal, and central and lateral lip were longest and widest in C. sieboldii, the shortest in C. discolor, and intermediate in C. bicolor. The spur length was the longest was C.
sieboldii was about 52%. This result was thought that these 2 species are almost genetically different species, because they have higher genetic diversity between 2 species than variants, and they have different morphological characteristics, such as flower color and flower size. The genetic position of variants is between C.
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