• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.

참고문헌 (26)

  1. Tomalia, D. A., H. Baker, J. Dewald, M. Hall, G. Kallos, S. Martin, J. Roeck, J. Ryder, and P. Smith (1985) A new class of polymers: starburst-dendritic macromolecules. Polymer J. 17: 117-132 
  2. Newkome, G. R., B. J. Childs, M. J. Rourk, G. R. Baker, and C. N. Moorefield (1998-1999) Dendrimer construction and macromolecular property modification via combinatorial methods. Biotechnol. Bioeng. 61: 243-253 
  3. Gupta, U., H. B. Agashe, A. Asthana, and N. K. Jain (2006) Dendrimers: novel polymeric nanoarchitectures for solubility enhancement. Biomacromolecules 7: 649-658 
  4. Lee, C. C., J. A. MacKay, J. M. J. Frechet, and F. C. Szoka (2005) Designing dendrimers for biological applications. Nat. Biotechnol. 23: 1517-1526 
  5. Song, C. K., B. W. Koo, and M. K. Jung (2001) Application of organic materials to electronic devices with respect to thin film transistor and sensor. Mol. Cryst. Liq. Cryst. 370: 337-342 
  6. Yoon, H. C. and H. S. Kim (2004) Bioelectrocatalyzed signal amplification for affinity interactions at chemically modified electrodes. Biotechnol. Bioprocess Eng. 9: 107-111 
  7. Svobodova, L., M. Snejdarkova, K. Toth, R. E. Gyurcsanyi, and T. Hianik (2004) Properties of mixed alkanethiol- dendrimer layers and their applications in biosensing. Bioelectrochemistry 63: 285-289 
  8. Bier, F. F., M. von Nickisch-Rosenegk, E. Ehrentreich- Forster, E. Reiss, J. Henkel, R. Strehlow, and D. Andresen (2008) DNA microarrays. Adv. Biochem. Eng. Biotechnol. 109: 433-453 
  9. Oh, S. J., B. J. Hong, K. Y. Choi, and J. W. Park (2006) Surface modification for DNA and protein microarrays. OMICS 10: 327-343 
  10. Campbell, C. T. and G. Kim (2007) SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics. Biomaterials 28: 2380-2392 
  11. Kim, Y. S., B. C. Kim , J. H. Lee , J. Kim, and M. B. Gu (2006) Specific detection of DNA using quantum dots and magnetic beads for large volume samples. Biotechnol. Bioprocess Eng. 11: 449-454 
  12. Lee, H. H. and P. Yager (2007) Microfluidic lab-on-achip for microbial identification on a DNA microarray. Biotechnol. Bioprocess Eng. 12: 634-639 
  13. Choi, J. W., B. K. Oh, Y. K. Kim, and J. Min (2007) Nanotechnology in biodevices. J. Microbiol. Biotechnol. 17: 5-14 
  14. Lee, S. W., W. J. Chang, R. Bashir, and Y. M. Koo (2007) 'Bottom-up' approach for implementing nano/ microstructure using biological and chemical interactions. Biotechnol. Bioprocess Eng. 12: 185-199 
  15. Caminade, A. M., C. Padie, R. Laurent, A. Maraval, and J. P. Majoral (2006) Uses of dendrimers for DNA microarrays. Sensors 6: 901-914 
  16. Benters, R., C. M. Niemeyer, and D. Wohrle (2001) Dendrimer-activated solid supports for nucleic acid and protein microarrays. Chembiochem 2: 686-694 
  17. Benters, R., C. M. Niemeyer, D. Drutschmann, D. Blohm, and D. Wohrle (2002) DNA microarrays with PAMAM dendritic linker systems. Nucleic Acids Res. 30: e10 
  18. Le Berre, V., E. Trevisiol, A. Dagkessamanskaia, S.Sokol, A. M. Caminade, J. P. Majoral, B. Meunier, and J. Francois (2003) Dendrimeric coating of glass slides for sensitive DNA microarrays analysis. Nucleic Acids Res. 31: e88 
  19. Park, J. W., Y. Jung, Y. H. Jung, J. S. Seo, and Y. Lee (2004) Preparation of oligonucleotide arrays with highdensity DNA deposition and high hybridization efficiency. Bull. Kor. Chem. Soc. 25: 1667-1670 
  20. Striebel, H. M., E. Birch-Hirschfeld, R. Egerer, Z. Foldes-Papp, G. P. Tilz, and A. Stelzner (2004) Enhancing sensitivity of human herpes virus diagnosis with DNA microarrays using dendrimers. Exp. Mol. Pathol. 77: 89-97 
  21. Borucki, M. K., J. Reynolds, D. R. Call, T. J. Ward, B. Page, and J. Kadushin (2005) Suspension microarray with dendrimer signal amplification allows direct and high-throughput subtyping of Listeria monocytogenes from genomic DNA. J. Clin. Microbiol. 43: 3255-3259 
  22. Yoon, H. C., M. Y. Hong, and H. S. Kim (2000) Affinity biosensor for avidin using a double functionalized dendrimer monolayer on a gold electrode. Anal. Biochem. 282: 121-128 
  23. Yam, C. M., M. Deluge, D. Tang, A. Kumar, and C. Cai (2006) Preparation, characterization, resistance to protein adsorption, and specific avidin-biotin binding of poly(amidoamine) dendrimers functionalized with oligo (ethylene glycol) on gold. J. Colloid. Interface Sci. 296: 118-130 
  24. Jung, Y. K., H. G. Park, and J. M. Kim (2006) Polydiacelylene (PDA)-based colorimetric detection of biotinstreptavidin interactions. Biosens. Bioelectron. 21: 1536-1544 
  25. Lee, C. S., S. H. Lee, Y. G. Kim, M. K. Oh, T. S. Hwang, Y. W. Rhee, H. M. Song, B. Y. Kim, Y. K. Kim, and B. G. Kim (2006) Fabrication of disposable protein chip for simultaneous sample detection. Biotechnol. Bioprocess Eng. 11: 455-461 
  26. Ajikumar, P. K., J. K. Ng, Y. C. Tang, J. Y. Lee, G. Stephanopoulos, and H. P. Too (2007) Carboxyl-terminated dendrimer-coated bioactive interface for protein microarray: High-sensitivity detection of antigen in complex biological samples. Langmuir 23: 5670-5677 

이 논문을 인용한 문헌 (1)

  1. 2009. "" Biotechnology and bioprocess engineering, 14(1): 6~12 


원문 PDF 다운로드

  • 원문 PDF 정보가 존재하지 않습니다.

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일