[논문]Laboratory and Field Evaluations of Entomopathogenic Lecanicillium attenuatum CNU-23 for Control of Green Peach Aphid (Myzus persicae)

Kim, Hyang-Yeon; Lee, Hyang-Burm; Kim, Young-Cheol; Kim, In-Seon

doi:10.4014/jmb.0800.235

Laboratory and Field Evaluations of Entomopathogenic Lecanicillium attenuatum CNU-23 for Control of Green Peach Aphid (Myzus persicae) 원문보기

Journal of microbiology and biotechnology, v.18 no.12, 2008년, pp.1915 - 1918

Kim, Hyang-Yeon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University) , Lee, Hyang-Burm (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University) , Kim, Young-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University) , Kim, In-Seon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally-friendly Agriculture Research Center, College of Agriculture and Life Sciences, Chonnam National University)

Abstract ▼ AI-Helper

An entomopathogenic fungus was isolated from an infected aphid. The isolate conformed most closely to Lecanicillium attenuatum CBS 402.78 (AJ292434) based on the internal transcribed spacer (ITS) region of its 18S rDNA, and thus was designated L. attenuatum CNU-23. Laboratory and field evaluations of CNU-23 blastospores were carried out for the control of green peach aphids. The laboratory evaluations of CNU-23 revealed an aphid mortality of about 80% with an estimated $LT_{50}$ of 3.72 days after the application of CNU-23 at $1{\times}10^6$ blastospores/ml. Meanwhile, the field evaluations of CNU-23 performed on greenhouse pepper plants during the rainy season showed an aphid mortality ranging from 72% to 97%. Significant sporulation was observed in the aphids treated with CNU-23. Therefore, the results suggest that L. attenuatum CNU-23 can be used as a biocontrol agent for green peach aphids on greenhouse pepper plants.

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Based on the phylogenetic analysis, the fungal isalate was most closely clustered with L. attenuatum CBS402.78 (AJ292434) retrieved from GenBank (Fig. 1), and the sequence identity values obtained from NCBI BLASTN searching were phylogenetically identical to the sequence of L. attenuatum CBS402.78 (AJ292434). Although the isolate was also related to other groups, including L.
The temperature and relative humidity ranged from 23 to 28℃ and 80% to 90%, respectively, during the experiments. The laboratory evaluations of CNU-23 showed that it was effective in controlling aphids when sprayed in a blastospore-based formulation, as about 80% aphid mortality was observed when CNU-23 was sprayed at IxlO₆ blastospores/ml (Fig. 3), whereas only 10% aphid mortality was observed in the control samples without the application of CNU-23. More than 60% aphid mortality was observed 4 days after the application of CNU-23, giving an LT50 value of 3.

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DOI : 10.4014/jmb.0800.235
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