Abstract

Quartz crystal microbalance immunosensor has a capacity to perform a label-free and real time detection of a trace amount of analyte through the specific interaction between antibody and antigen. However, immobilization of antibody molecules on the sensor surface is a troublesome procedure for researchers who are not experienced in chemistry. Protein G has a specific affinity to antibody and would serve as a capturing agent for antibody when immobilized on the sensor surface. In this work, we prepared a protein G sensor chip by immobilizing protein G on the surface of quartz crystal microbalance and examined its capability to detect human haptoglobin or human transferrin with unpurified corresponding antiserum. Specific and dose dependent response was observed when the protein G chip was used for detection of antigens after saturated with antiserum. We also verified several advantageous aspects of the protein G chip such as improved flexibility and sensitivity.

주제어

#protein G   #immunosensor   #quartz crystal microbalance(QCM)   #haptoglobin   #transferrin  

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이 논문을 인용한 문헌 (2)

1. Kim, Sung-Il ; Ha, In-Young ; Choi, Suk-Jung 2009. "Detection of human and bovine haptoglobin by using quartz crystal microbalance sensor chip containing secondary antibody" 센서학회지 = Journal of the Korean Sensors Society, 18(2): 160~167  원문보기 상세보기
2. Sin, Eun-Jung ; Sohn, Young-Ho ; Sohn, Young-Soo 2010. "A comparison of detection capabilities of anti-IgG immobilizedby protein G and NHS" 센서학회지 = Journal of the Korean Sensors Society, 19(2): 142~148  원문보기 상세보기