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논문 상세정보

Abstract

To develop a whitening agent, cytotoxicity of the soluble collagen isolated from Todarodes pacificus (CIT) was evaluated. CIT tested for cytotoxicity on human dermal fibroblast (CCD-986sk) was exhibited very low cytotoxicity. Because tyrosinase is the key enzyme for melanin biosynthesis, the use of various tyrosinase inhibitors is a common practice for whitening purpose in cosmetics. Tyrosinase inhibitory activity and melanin production assay were measured to confirm the whitening effect. The inhibitory effect of MMP-1 in UV-irradiated human dermal fibroblast was also performed. CIT showed strong inhibition potency on tyrosinase by 51.5% at 0.2 mg/mL which increased the inhibition by increasing the concentration of CIT, and showed 69.1% inhibition at 1.0 mg/mL. CIT showed strong inhibition effect on melanin production with 82% at 1.0 mg/mL. The CIT also reduced about 76% expression of MMP-1 in UV-irradiated CCD-986sk cell at 1.0 mg/mL. From the preliminary observations, we suggest that the collagen isolated from CIT could be a potential source of natural skin-whitening and anti-aging agents for the photo-damaged skin.

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  13. Kim, Han-Soo ; Jang, Seong-Ho ; Yoon, Myung-Joo ; Kang, Jin-Soon ; Choi, Woo-Seok 2011. "Improvements Caused by Chitosan, Sericin and Collagen Peptide Extract Complexes on Lipid Metabolism in Dyslipidemia" 한국환경과학회지 = Journal of the environmental sciences, 20(8): 1021~1030 
  14. Kim, Han-Soo ; Jang, Seong-Ho ; Yoon, Myung-Joo ; Choi, Woo-Seok ; Kang, Jin-Soon 2011. "Influences of Chitosan, Sericin and Collagen Extract Complexes on the Improvement Actions of Lipid Component in Diabetes" 한국환경과학회지 = Journal of the environmental sciences, 20(8): 1031~1039 
  15. Yang, Su Jin ; Hong, Joo-Heon 2012. "Extraction and Physicochemical Properties of Collagen from Squid (Todarodes pacificus) skin and Alaska pollack (Theragra chalcogramma) skin" 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 28(6): 711~719 
  16. Yang, Su-Jin ; Hong, Joo-Heon 2014. "Physicochemical Properties and Biological Activities of Collagens with Different Molecular Weights from Alaska Pollack (Theragra chalcogramma) Skin" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 43(10): 1535~1542 

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