[논문]인플루엔자 바이러스에 대한 Zanthoxylum속의 항바이러스 효과 검정

최화정; 송재형; 권두한; 백승화; 안용준

인플루엔자 바이러스에 대한 Zanthoxylum속의 항바이러스 효과 검정
Antiviral Activity of Zanthoxylum Species against Influenza Virus 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.16 no.4, 2008년, pp.273 - 278

최화정 (한국생명공학연구원 천연물의약연구센터) , 송재형 (원광대학교 한의학전문대학원) , 권두한 (한국생명공학연구원 천연물의약연구센터) , 백승화 (원광대학교 한의학전문대학원) , 안용준 (서울대학교 농생명과학대학)

Abstract ▼ AI-Helper

We aimed to investigate the antiviral activity of Zanthoxylum species against influenza virus A/WS/33, A/PR/8 and B/Lee/40 used by sulforhodamine B (SRB) assay and the action of leaves extracts of Zanthoxylum piperitum on life cycle of influenza virus A/WS/33. Among the twelve extracts, only the leaf extract of Z. piperitum exhibited strong antiviral activity at low concentration of less than 10${\mu}g/m{\ell}$ with no citotoxicity (50${\mu}g/m{\ell}$) against all of three viruses. In addition, only oseltamivir showed antiviral activity with $IC_{50}$ of 65.3${\mu}g/m{\ell}$ against influenza A/WS/33 among the viruses. Furthermore, the leaf extract of Z. piperitum suppressed infection of influenza virus A/WS/33, when added just prior (-1 hr) or after virus inoculation (0 hr). Leaf extract of Z. piperitum directly affect the infectivity of influenza virus A/WS/33 particles. Therefore, Leaf extract of Z. piperitum exhibited higher antiviral activity against three influenza viruses than that of the oseltamivir, which directly interacts with influenza A/WS/33 particles, affecting the initial stages of infection such as receptor binding and virus entry.

주제어

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문제 정의

The objective of this study was to determine whether methanol extracts of different plant tissues would function as an antiviral agent against influenza A or B virus in vitro. Subsequently, we also attempted to elucidate the ability of Z.

제안 방법

, 1997). In this study, we investigated the antiviral activity of four plants from the Zanthoxylum species against influenza viruses A/WS/33, A/PR/8 and B/Lee/40 via sulforhodamine B (SRB) assay, and the action of leaf extract of Z. piperitum on the life cycle of influenza virus A/WS/33.
To study the inhibitory effect of Z. piperitum leaf extract, which possess a broad spectrum of antiviral activities on the stages of virus infection, the compounds were added at different periods (before, during, and after) of influenza virus A/WS/33 infection. Results showed that the leaf extract suppressed influenza virus A/WS/33 infection, when added just prior (−1 hr) or after the virus inoculation (0 hr).

대상 데이터

Four plants, Zanthoxylum piperitum, Zanthoxylum schinifolium, Zanthoxylum coreanum, and Zanthoxylum planispinum were collected from Mt. Gwanggyo (Suwon, Korea), air-dried in room temperature and pulverized. Each 100g sample of the specimen plants was extracted twice with 600㎖ of methanol at room temperature for 3 days and filtered (Whatman No.
Louis, MO, USA). Oseltamivir (F. Hofmann-La Roche Ltd, Switzerland) were purchased from a pharmacy in Korea as prescribed by a medical doctor. All other chemicals were a reagent grade.

이론/모형

1 to 100 ㎍/㎖. A ten-fold dilution scheme for each compound was employed. Three wells were used as virus controls (virusinfected non-drug-treated cells) while three wells were used as cell controls (non-infected non-drug-treated cells).

성능/효과

Analysis was performed to ascertain whether leaf extract of Z. piperitum interact with the viral particle, Antiviral activity of pre-incubation with leaf extract of Z. piperitum resulted in 94%. Similarly, continuous presence of the extract during infection led to 93% antiviral activity (Fig.
piperitum exhibited antiviral activity against influenza viruses A/WS/33, A/PR/8 and B/Lee/40, higher than that of oseltamivir. Our results showed that leaf extract of Z. piperitum inhibit the growth of influenza strains A/WS/33, A/PR/8 and B/Lee/40 with differences in inhibitory efficacy among the strains.
piperitum leaf extract, which possess a broad spectrum of antiviral activities on the stages of virus infection, the compounds were added at different periods (before, during, and after) of influenza virus A/WS/33 infection. Results showed that the leaf extract suppressed influenza virus A/WS/33 infection, when added just prior (−1 hr) or after the virus inoculation (0 hr). The inhibitory rate was higher than 60% (Fig.

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