소 결핵은 만성적으로 서서히 진행 되어 쇠약해 지기 때문에 일반적으로 사후 검시 후에 확인 된다. 소결핵의 감염이 대부분 폐에서 발생하기 때문에 심한 경우에는 기침, 비루 및 호흡 곤란을 유발 한다. 본 고에서는 도축장에서 나온 사체의 심장, 간, 신장, 폐, 복수, 임파선 및 내장에서 얻은 시료를 분석한 것이다. 사체의 부검 검시도중 다양한 모양과 크기의 결핵 병변이 관찰 되었다. 백색 크림 색깔의 결핵은 화농성으로 임파선, 폐 및 장간막을 포함하여 흉막강에 퍼져 있었다. 폐와 임파선의 그램 염색과 Ziehl-Neelsen's acid-fast 염색 결과 강한 조직화학적 양성 반응을 나타내었다. Acid-fast에 염색된 유기체들이 현미경 검경에서 발견 되었다. 본 고에서는 의심원인체로 사료되는 Mycobacterium bovis의 조직학적 발견을 근거로 이에 의한 소결핵의 조직병리학적 소견을 보고하는 바이다.
소 결핵은 만성적으로 서서히 진행 되어 쇠약해 지기 때문에 일반적으로 사후 검시 후에 확인 된다. 소결핵의 감염이 대부분 폐에서 발생하기 때문에 심한 경우에는 기침, 비루 및 호흡 곤란을 유발 한다. 본 고에서는 도축장에서 나온 사체의 심장, 간, 신장, 폐, 복수, 임파선 및 내장에서 얻은 시료를 분석한 것이다. 사체의 부검 검시도중 다양한 모양과 크기의 결핵 병변이 관찰 되었다. 백색 크림 색깔의 결핵은 화농성으로 임파선, 폐 및 장간막을 포함하여 흉막강에 퍼져 있었다. 폐와 임파선의 그램 염색과 Ziehl-Neelsen's acid-fast 염색 결과 강한 조직화학적 양성 반응을 나타내었다. Acid-fast에 염색된 유기체들이 현미경 검경에서 발견 되었다. 본 고에서는 의심원인체로 사료되는 Mycobacterium bovis의 조직학적 발견을 근거로 이에 의한 소결핵의 조직병리학적 소견을 보고하는 바이다.
Bovine tuberculosis is generally detected postmortem because it is a chronic debilitating disease. Since tuberculosis is mainly found in the lungs, clinical signs including coughing, nasal discharge, and difficulty breathing can occur in severe instances. In the present study, specimens were collect...
Bovine tuberculosis is generally detected postmortem because it is a chronic debilitating disease. Since tuberculosis is mainly found in the lungs, clinical signs including coughing, nasal discharge, and difficulty breathing can occur in severe instances. In the present study, specimens were collected from the heart, liver, kidney, lung, pleural cavities, lymph nodes and intestines of carcasses found in an abattoir. According to post-mortem examination and inspection of carcasses, tuberculosis lesions were varied in appearance and size. Tubercles of a white cream color were disseminated throughout the pleural cavity including the lymph nodes, lungs and mesentery containing pus. Gram and Ziehl-Neelsen's acid-fast staining for the lung and lymph nodes revealed a highly positive histochemical reaction. The acid-fast organisms were observed histologically in the lesions under a microscope. This report demonstrated the histopathology of bovine tuberculosis based on the histological findings of Mycobacterium bovis, which is a suspected causative agent.
Bovine tuberculosis is generally detected postmortem because it is a chronic debilitating disease. Since tuberculosis is mainly found in the lungs, clinical signs including coughing, nasal discharge, and difficulty breathing can occur in severe instances. In the present study, specimens were collected from the heart, liver, kidney, lung, pleural cavities, lymph nodes and intestines of carcasses found in an abattoir. According to post-mortem examination and inspection of carcasses, tuberculosis lesions were varied in appearance and size. Tubercles of a white cream color were disseminated throughout the pleural cavity including the lymph nodes, lungs and mesentery containing pus. Gram and Ziehl-Neelsen's acid-fast staining for the lung and lymph nodes revealed a highly positive histochemical reaction. The acid-fast organisms were observed histologically in the lesions under a microscope. This report demonstrated the histopathology of bovine tuberculosis based on the histological findings of Mycobacterium bovis, which is a suspected causative agent.
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성능/효과
Histochemical analysis, polymerase chain reaction (PCR) and tissue culture are necessary for the rapid and accurate diagnosis. In this study, histological and histochemical analyses for bovine TB were demonstrated in various organ/ tissue of abattoir cases, suggesting bovine TB is caused by M. bovis infection that is the important zoonotic threat with highly infectious in human by causing similar diseases to that by M. tuberculosis.
참고문헌 (22)
Araujo, C. P., C. Q. Leite, K. A. Prince, Kdos S. Jorge, and A. L. Osorio. 2005. Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do SuI, Brazil. Mem. Inst. Oswaldo. Cruz. 100, 749-752
Briones, V., L. de Juan, c. Sanchez, A. I. Vela, M. Galka, Montero, J. Goyache, A. Aranaz, and L. Dominguez. 2000. Bovine tuberculosis and the endangered Iberian lynx. Emerg. Infect. Dis. 6, 189-191
Cosivi, O., J. M. Grange, C. J. Daborn, M. C. Raviglione, T. Fujikura, D. Cousins, R A. Robinson, H. F. Huchzermeyer, I. de Kantor, and F. X. Meslin. 1998. Zoonotic tuberculosis due to Mycobacterium bovis in developing countries. Emerg. Infect. Dis. 4, 59-70
Denis, M., D. N. Wedlock, and B. M. BuddIe. 2005. Mycobacterium bovis infection of bovine macrophages induces cytokine release: enhancement by interferon-gamma and relationship between cytokine-induced macrophage activation and apoptosis. Immunol. Cell BioI. 83, 641-653
Doherty, M. L., H. F. Bassett, P. J. Quinn, W. C. Davis, A. P. Kelly, and M. L. Monaghan. 1996. A sequential study of the bovine tuberculin reaction. Immunology 87, 9-14
Fend, R., R. Geddes, S. Lesellier, H. M. Vordermeier, L. A. Corner, E. Gormley, E. Costello, R. G. Hewins on, D. J. Marlin, A. C. Woodman, and M. A. Chambers. 2005. Use of an electronic nose to diagnose Mycobacterium bovis infection in badgers and cattle. J. Clin. Microbiol. 43, 1745-1751
Francis, J., R. J. Seiler, I. W. Wilkie, D. O'Boyle, M. J. Lumsden, and A. J. Frost. 1978. The sensitivity and specificity of various tuberculin tests using bovine PPD and other tuberculins. Vet. Rec. 103, 420-425
Grooms, D. and J. Molesworth. 2000. Caudal-fold tuberculin Test. Extension Bulletin E-2730, Michigan State University, College of Veterinary Mdeicine. 1-2
Herenda, D. 2000. Manual on meat inspection for developing countries. Food and Agriculture Organization of the United Nations (FAO) Animal production and health paper 119. (www.fao.org/docrep)
Kao, R. R., M. G. Roberts, and T. J. Ryan. 1997. A model of bovine tuberculosis control in domesticated cattle herds. Proc. BioI. Sci. 264, 1069-1076
Lutze-Wallace, C. and C. Turcotte. 2005. Laboratory diagnosis of bovine tuberculosis in Canada for calendar year. Can. Vet. J. 47, 871-873
Lyashchenko, K. 2004. Novel Immunoassay Technologies for Serological Detection of Bovine Tuberculosis. Activities Report & Proceedings of the 2004 Bovine Tuberculosis Conference. pp. 26
Mathews, F., D. W. Macdonald, G. M. Taylor, M. Gelling, R. A. Norman, P.E. Honess, R. Foster, C. M. Gower, S. Varley, A. Harris, S. Palmer, G. Hewins on, and J. P. Webster. 2006. Bovine tuberculosis (Mycobacterium bovis) in British farmland wildlife: the importance to agriculture. Proc. BioI. Sci. 273, 357-365
Palmer, M. V., D. L. Whipple, J. C. Rhyan, C. A. Bolin, and D. A. Saari. 1999. Granuloma development in cattle after intratonsilar inoculation with Mycobacterium bovis. Am. J. Vet. Res. 60, 310-315
Pollock, J. M., J. McNair, H. Bassett, J. P. Cassidy, E. Costello, H. Aggerbeck, I. Rosenkrands, and P. Andersen. 2003. Specific delayed-type hypersensitivity responses to ESAT-6 identify tuberculosis-infected cattle. J. Clin. Microbiol. 41, 1856-1860
Scantlebury, M., M. R. Hutchings, D. J. Allcroft, and S. Harris. 2004. Risk of disease from wildlife reservoirs: badgers, cattle, and bovine tuberculosis. J. Dairy. Sci. 87, 330-339
Smith, R. M., F. Drobniewski, A. Gibson, J. D. Montague, M. N. Logan, D. Hunt, G. Hewinson, R. L. Salmon, and B. O'Neill. 2004. Mycobacterium bovis infection, United Kingdom. Emerg. Infect. Dis. 10, 539-541
Steele, J. H. 1995. Regional and country status report-Introduction. pp. 46-61. In Thoen, C. O. and J. H. Steele (eds.), Mycobacterium bovis infection in animals and humans. Iowa State University Press. Ames Iowa 50011 in USA
Timoney, J. F., J. H. Gillespie, F. W. Scott, and J. F. Barlough. 1988. Hagan and Bruner's Microbiology and Infectious Diseases of Domestic Animals. pp. 295-317, 8th eds
Widmer, A. F. and R Frei. 1999. Decontamination, disinfection and sterilization. pp. 138-164, In Murray, P. R, E. J. Baron, M. A. Pfaller, F. Tenover, and R H. Yolker (eds.), Manual of Clinical Microbiology, American Society for Microbiology, Washington
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