닭의 품종 기원을 결정하거나 유전적 변이의 정도를 확인 하는데 미토콘드리아 DNA D-loop 염기서열을 이용하여 오고 있다. 본 연구는 한국재래계 갈색종과 흑색종, 로드아일랜드레드종, 코니쉬종의 4품종 41개체의 염기서열을 분석함으로 품종간의 유전적 연관관계를 확인하였다. 그 결과 총 10개의 haplotype를 확인할 수 있었으며, haplotype 1과 2는 가장 많은 수인 8개체씩이 포함되었다. 계통도 분석을 통해 한국재래계 흑색종과 갈색종은 haplotype 2를 모두 가지고 있는 것으로 확인되었으며, 이 haplotype은 적색야계와 유전적으로 가깝게 위치한 것을 알 수 있었다. 본 연구를 통해 D-loop 염기서열 변이가 품종 판별 마커로 이용 가능성이 있는지 확인하였다. 그 결과 여러 단일염기다형 마커의 조합으로 품종의 구분이 가능할 것으로 추정되며, 앞으로 더 많은 연구가 진행되어야 할 것으로 생각된다. 이 연구의 결과는 한국재래계의 보존 및 육종계획 수립과 더불어 품종판별 마커의 개발의 기초 자료를 제공할 것으로 생각된다.
닭의 품종 기원을 결정하거나 유전적 변이의 정도를 확인 하는데 미토콘드리아 DNA D-loop 염기서열을 이용하여 오고 있다. 본 연구는 한국재래계 갈색종과 흑색종, 로드아일랜드레드종, 코니쉬종의 4품종 41개체의 염기서열을 분석함으로 품종간의 유전적 연관관계를 확인하였다. 그 결과 총 10개의 haplotype를 확인할 수 있었으며, haplotype 1과 2는 가장 많은 수인 8개체씩이 포함되었다. 계통도 분석을 통해 한국재래계 흑색종과 갈색종은 haplotype 2를 모두 가지고 있는 것으로 확인되었으며, 이 haplotype은 적색야계와 유전적으로 가깝게 위치한 것을 알 수 있었다. 본 연구를 통해 D-loop 염기서열 변이가 품종 판별 마커로 이용 가능성이 있는지 확인하였다. 그 결과 여러 단일염기다형 마커의 조합으로 품종의 구분이 가능할 것으로 추정되며, 앞으로 더 많은 연구가 진행되어야 할 것으로 생각된다. 이 연구의 결과는 한국재래계의 보존 및 육종계획 수립과 더불어 품종판별 마커의 개발의 기초 자료를 제공할 것으로 생각된다.
In order to determine the origin and genetic diversity among chicken breeds, mitochondrial (mt) DNA D-loop sequences have been widely used. In this study, 41 individuals from four breeds (Korean native chicken (Black and Brown) and two imported breeds, Rhode Island Red and Cornish) were used for ide...
In order to determine the origin and genetic diversity among chicken breeds, mitochondrial (mt) DNA D-loop sequences have been widely used. In this study, 41 individuals from four breeds (Korean native chicken (Black and Brown) and two imported breeds, Rhode Island Red and Cornish) were used for identifying genetic relationships with other chicken breeds. We obtained ten haplotypes and the highest number of haplotype was represented by eight individuals each from haplotype 1 and haplotype 2. Neighbor-joining phylogenetic tree indicates that the black and brown Korean native chicken breeds were mixed in haplotype 2 and they were closely related with the red jungle fowl (Gallus gallus). We also investigated whether the D-loop hypervariable region in chicken mtDNA can be used for the breed identification marker. The results indicated that the combination of the SNPs in the D-loop region can be possibly used for the breed discriminating markers. The results obtained in this study can be used for designing proper breeding and conservation strategies for Korean native chicken, as well as development of breed identification markers.
In order to determine the origin and genetic diversity among chicken breeds, mitochondrial (mt) DNA D-loop sequences have been widely used. In this study, 41 individuals from four breeds (Korean native chicken (Black and Brown) and two imported breeds, Rhode Island Red and Cornish) were used for identifying genetic relationships with other chicken breeds. We obtained ten haplotypes and the highest number of haplotype was represented by eight individuals each from haplotype 1 and haplotype 2. Neighbor-joining phylogenetic tree indicates that the black and brown Korean native chicken breeds were mixed in haplotype 2 and they were closely related with the red jungle fowl (Gallus gallus). We also investigated whether the D-loop hypervariable region in chicken mtDNA can be used for the breed identification marker. The results indicated that the combination of the SNPs in the D-loop region can be possibly used for the breed discriminating markers. The results obtained in this study can be used for designing proper breeding and conservation strategies for Korean native chicken, as well as development of breed identification markers.
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가설 설정
Another one individual (Kbl-3) belongs to the haplotype 4, which also contains brown Korean native chicken and Cornish breeds. Large numbers of Korean native chicken (both black and brown) individuals have the haplotype 2. These in- dividuals are closely related with red jungle fowl and Thailand chicken breeds. This also indicates that the Korean native chicken is more closely related with the ancient chicken breeds compared with the other commercial breeds
제안 방법
5 mM each of dATP, dCTP, dGTP and dTTP), 1 μL of 10 pM of each primer and 1 U HS Prime Taq (GeNet Bio, Korea) in a 25 μL reaction volume. The PCR reaction was performed in a My- Genie96 Thermal Block (Bioneer, Korea) with an initial denaturation step at 94℃ for 10 min followed by 35 cycles of 30 sec at 94℃, 30 sec at 61℃, 40 sec at 72℃ and a final extension step at 72℃ for 10 min. Purification of PCR products was per formed using AccuprepⓇ PCR purification kit (Bioneer, Korea) according to the manufacturer’s instructions.
이론/모형
DNAs were extracted by using the procedures described by the manufacturer’s standard procedure of G-DexTM Genomic DNA Extraction Kit (iNtRON Biotechnology, Inc).
The chicken mtDNA D-loop nucleotide sequences obtained in this study were aligned using the ClustalW program (Thomp- son et al., 1994) and saved as bioedit format. Nucleotide replace- ment export data (Fig.
성능/효과
Analysis of sequences from D-loop hypervariable region in chicken mtDNA identified a total of 23 nucleotide substitutions, which were classified as ten haplotypes (Table 1). The highest common haplotype group contains eight individuals for both haplotype 1 and haplotype 2.
No deletion or insertion was de-tected in our sequence analysis. Based on our study, brown Ko-rean native chicken breed has 7 different haplotypes (haplotype 1, 2, 4, 5, 8, 9, 10), black Korean native chicken and Cornish breed have only 3 haplotypes each (haplotype 2, 4, 6 for black Korean native chicken) and (haplotypes 3, 4, 5 for Cornish). On the other hand, Rhode Island Red has only one haplotype (ha-plotype 1).
In conclusion, the phylogenetic analysis of four chicken breeds containing black Korean native chicken, brown Korean native chicken, Rhode Island Red and Cornish breeds using D-loop region in mtDNA indicated that Korean native chickens have relationship with the major lineage of Red jungle fowl. The results presented here can give the broad idea for the phylogenetic relationship of Korean native chicken and provide opportunities for the development of breed specific markers.
On the other hand, Rhode Island Red has only one haplotype (ha-plotype 1). Therefore, we can identify that the haplotype 2 is the common haplotype for the Korean native chicken and the unique haplotype 6 and 7 were identified in black Korean na-tive chicken and brown Korean native chicken, respectively and haplotype 4 is the common haplotype for the three breeds in-vestigated in this study.
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