$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

Tannic acid와 Bacillus thuringiensis subsp. kurstaki KB100균주의 혼합처리에 의한 파밤나방 살충활성의 상승효과

The Synergy Effects of Mixed Treatment with Tannic Acid and Bacillus thuringiensis subsp. kurstaki KB100 against Spodoptera exigua (Lepidoptera: Noctuidae)

초록

국내 토양으로부터 파밤나방에 활성있는 Bacillus thuringiensis subsp. kurstaki KB100균주를 분리 동정하였다. 난방제 해충인 파밤나방에 대한 B. thuringiensis의 살충력을 강화시키기 위해 몇 개의 protease inhibitor를 조사하였으며, 이 중 단백질 분해 억제 능력이 가장 좋은 tannic acid을 선발하였다. B. thuringiensis의 배양액에 tannic acid을 혼합 처리하여 파밤나방 2령 유충을 대상으로 생물활성을 조사한 결과, B. thuringiensis 단독처리는 54.4%의 사충율을 보인 반면 B. thuringiensis와 4 mM tannic acid을 혼합하여 처리하면 64.0% 그리고 B. thuringiensis+40 mM tannic acid는 95.5%의 높은 상승효과를 각각 나타냈다. 그러나 B. thuringiensis+80 mM tannic acid 에서는 53.3%로 단독처리의 경우 보다 사충율이 낮게 나타났다. 3령 유충에서도 B. thuringiensis 단독처리는 60.0%였으며 B. thuringiensis+40 mM tannic acid는 93.3%로 사충율이 증가하는 상승효과를 보였다. 야외포장 실험에서 B. thuringiensis 단독처리는 1회, 2회, 3회 누적 처리에서 각각 61.8%, 80.4% 그리고 47.3%의 사충율을 나타낸 것에 비해 B. thuringiensis와 40 mM tannic acid의 혼합 처리구에서는 83.9%, 89.4%와 66.8%로 사충율이 증가되었다.

Abstract

Bacillus thuringiensis subsp. kurstaki KB100 isolated from the domestic soil have the most effective activity against the beet armyworm, Spodoptera exigua larva. The tannic acid as protease inhibitor might be increased the efficacy of sublethal concentrations of B. thuringiensis. The tannic acid was identified as a protease inhibitor that could increased the efficacy of sublethal concentrations of B. thuringiensis. Mixture of B. thuringiensis and tannic acid was investigated the mortality of S. exigua larva in the laboratory and field. When B. thuringiensis treated to 2nd larva of S. exigua, mortality was shown 54.4%. However, mixtures of B. thuringiensis with 4 and 40 mM tannic acid were increased mortalities to 2nd larva of S. exigua as 64.0 and 95.5%, respectively. Also, synergy effect of mixture of B. thuringiensis and 40 mM tannic acid was increased the mortality of S. exigua 3rd larva to 93.3%, even though 60.0% mortality with only B. thuringiensis treatment. On the other hand, the mortality of mixture with B. thuringiensis and 80 mM tannic acid was 53.3% lower than B. thuringiensis single treatment. In the welsh onion field, the accumulated mortalities of 3 times replicated with mixture of B. thuringiensis and 40 mM tannic acid were 83.9, 89.4 and 66.8% compare with 61.8, 80.4 and 47.3% as only B. thuringiensis treatment, respectively.

참고문헌 (23)

  1. Ahn, S.B., I.S. Kim, W.S. Cho, M.H. Lee and K.M. Choi. 1989. The Occurance of the crop insect pests from Korea in 1988. Korean J. Appl. Entomol. 28(4): 246-253 
  2. Bartelt, R.J., M.R. Mc Guire and D.A. Black. 1990. Feeding stimulants for the European corn borer(Lepidoptera pyralidae): Additives to a starch-based formulation of Bacillus thuringiensis. Environ. Entomol. 19: 182-189 
  3. Bradford, M.M. 1976. A Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 
  4. Crickmore, N., D.R. Zeigler., J. Feitelson., E. Schnepf., J. van Rie., D. Lereclus., J. Baum and J.H. Dean. 1998. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal protein. Microbiol. Mol. Biol. Rev. 62: 807-813 
  5. El-Nockrashy, A.S., H.S. Salama and F. Taha. 1986. Influence of bait formulations on the effectiveness of Bacillus thuringiensis against Spodoptera littoralis (Boisd) (lepidoptera:Nocutidae). J. Appl. Entomol. 101: 389-399 
  6. Eveleens, K.G., R. Van Den Bosch and L.E. Ehler. 1973. Secondary outbreak induction of beet armyworm by experimental insecticide application in cotton in California. Entomol. 2: 497-503 
  7. Finney, D.J. 1971. Probit analysis, estimation of the median effective dose. Cambridge University Press. London. pp 19-47 
  8. Goh, H.G., J.D. Park, Y.M. Choi, K.M. Choi and I.S. Park. 1991. The host plants of beet armyworm, Spodoptera exigua (Hubner), (Lepidoptera: Noctuidae) and its occurrence. Korean J. Appl. Entomol. 30(2): 111-116 
  9. Goh, H.G., J.S. Choi, K.B. Uhm, K.M. Choi and J.W. Kim. 1993a. Spatial distribution pattern of beet armyworm, Spodoptera exigua (Hübner), larvae in the welsh onion field. Korean J. Appl. Entomol. 32(2): 134-138 
  10. Goh, H.G., J.S. Choi, K.B. Uhm, K.M. Choi and J.W. Kim. 1993b. Seasonal fluctuation of beet armyworm, Spodoptera exigua (Hubner), adult and larva. Korean J. Appl. Entomol. 32(4): 389-394 
  11. Goh, H.G., S.G. Lee, B.P. Lee, K.M. Choi and J.H. Kim. 1990. Simple mass-rearing of beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae), on an artificial diet. Korean J. Appl. Entomol. 29: 180-183 
  12. Kim, D.A., J.S. Kim., M.R. Kil., S.K. Paek., S.Y. Choi., D.Y. Jin., Y.N. Youn., I.C. Hwang., Y.M. Yu. 2008. Characterization of new Bacillus thuringiensis isolated with bioactivities to tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae). Korean J. Appl. Entomol. 47(1): 87-93 
  13. Kim, H.S., D.W. Lee, H.W. Park, Y.M. Yu, J.I. Kim and S.K. Kang. 1995a. Distribution and characterization of Bacillus thuringiensis isolated form soils of sericultural farms in Korea. Korean J. Seric. Sci. 34(1): 57-61 
  14. Kim, K.C., J.D. Park and D.S. Choi. 1995b. Seasonal occurrence of Spodoptera exigua in Chonnam province and a possibility of their control in vinyl house with pheromone trap. Korean J. Appl. Entomol. 34(2): 106-111 
  15. Kim, Y. and N. Kim. 1997. Cold hardiness of the beet armyworm, Spodoptera exigua (Noctuidae: Lepidoptera). Environ Entomol. 26: 1117-1123 
  16. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680-685 
  17. Luo, L.Z., Y.Z. Cao and X.F. Jiang. 2000. The study on occurrence characteristics and trend of beet armyworm. Plant Protection. 26(3): 37-39 
  18. Maclntosh, S.C., G.M. Koshore, F.J. Perlak, P.G. Marrone, T.B. Stone, S.R. Sims and R.L. Fuchs. 1990. Potemtiation of Bacillus thuringiensis insecticidal activity by serine protease inhibitor. J. Agric. Food Chem. 38: 1145-1152 
  19. Opprert, B. 1999. Protease interaction with Bacillus thurigiensis insecticidal toxin. Arch. Insect Biochem. Physiol. 42: 1-12 
  20. Park, J.D., H.G. Goh, J.H. Lee, W.J. Lee and K.J. Kim. 1991. Flight activity and injury characteristics of beet armyworm, Spodoptera exigua (Hubner), (Lepidoptera: Noctuidae) in Southern Region of Korea. Korean J. Appl. Entomol. 30(2): 124-129 
  21. Raubenheimer, D. 1992. Tannic acid, protein, and digestible carbohydrate: Dietary imbalance and nutritional compensation in locusts. Ecology 73: 1012-1027 
  22. Raymond, M. 1985. Presentation d'un programme d'analyse log-probit pour micro-ordinnateur. Cah. ORSTOM, Ser. Ent. Med. et parasitol. 22: 117-121 
  23. Satinder K.B., M. Verma, R.D. Tyagi, R.Y. Surampalli, S. Barnabe, J.R. Valero. 2007. Bacillus thuringiensis proteases: production and role in growth, sporulation and synergism. Process Biochem. 42: 773-790 

이 논문을 인용한 문헌 (4)

  1. Park, Jung-A ; Seok, Jung-Kyun ; Prasad, Surakasi Venkara ; Kim, Yong-Gun 2011. "Sound Stress Alters Physiological Processes in Digestion and Immunity and Enhances Insecticide Susceptibility of Spodoptera exigua" 한국응용곤충학회지 = Korean journal of applied entomology, 50(1): 39~46 
  2. Han, Ji Hee ; Kim, Hyeonggyeong ; Leem, Hun Tae ; Kim, Jeong Jun ; Lee, SangYeob 2013. "Characteristics and Virulence Assay of Entomopathogenic Fungus Metarhizium anisopliae for the Microbial Control of Spodoptera exigua" 농약과학회지 = The Korean journal of pesticide science, 17(4): 454~459 
  3. Han, Ji Hee ; Kim, Jeong Jun ; Lee, SangYeob 2014. "Insecticidal Activity of Metarhizium anisopliae FT83 against the Different Stages of Beet Armyworm, Spodoptera exigua" 농약과학회지 = The Korean journal of pesticide science, 18(4): 417~421 
  4. Park, Jong-Ho ; Hong, Sung-Jun ; Han, Eun-Jung ; Shim, Chang-Ki ; Kim, Min-Jeong ; Kim, Yong-Ki 2015. "Control Efficacy of Entomopathogenic Microagent against Spodoptera exigua on Organic Chinese Cabbage" 韓國有機農業學會誌 = Korean journal of organic agriculture, 23(4): 797~811 

DOI 인용 스타일