본 연구에서는 골프장에서 문제가 되는 주요 병의 방제가능한 생물학적 제제를 개발하기 위하여 실시하였다. 강원도 소재 골프장의 잔디 근권토양으로부터 채취한 100여개의 미생물로부터 4개의 길항 미생물을 선발하였다. 배지를 이용한 저지원 실험에서 4개의 균주는 한국 골프장에서 문제가 되고 있는 옐로우패취(Rhizoctonia cerealis), 브래운패취(R. solani AG-1(1B)), 동전마름병(Sclerotinia homoeocarpa), 그리고 설부갈색소립균핵병원균 (Typhula incarnata)에 대하여 효과적이었다. 옐로우패취, 브래운패취, 동전마름병을 대상으로 온실실험 결과, 4개의 균주는 병의 종류, 기주 식물체 및 병 발생 정도에 따라 효과가 달랐지만 한개 혹은 그 이상의 병에 대해 60% 이상을 방제가를 기록하였다. 4개의 균주는 생화학적 특성과 16S rRNA 분석을 통해 Pseudomonas spp.로 밝혀졌다. 현재 4개의 균주는 적정 배양조건과 제제화를 위한 연구 중에 있다.
본 연구에서는 골프장에서 문제가 되는 주요 병의 방제가능한 생물학적 제제를 개발하기 위하여 실시하였다. 강원도 소재 골프장의 잔디 근권토양으로부터 채취한 100여개의 미생물로부터 4개의 길항 미생물을 선발하였다. 배지를 이용한 저지원 실험에서 4개의 균주는 한국 골프장에서 문제가 되고 있는 옐로우패취(Rhizoctonia cerealis), 브래운패취(R. solani AG-1(1B)), 동전마름병(Sclerotinia homoeocarpa), 그리고 설부갈색소립균핵병원균 (Typhula incarnata)에 대하여 효과적이었다. 옐로우패취, 브래운패취, 동전마름병을 대상으로 온실실험 결과, 4개의 균주는 병의 종류, 기주 식물체 및 병 발생 정도에 따라 효과가 달랐지만 한개 혹은 그 이상의 병에 대해 60% 이상을 방제가를 기록하였다. 4개의 균주는 생화학적 특성과 16S rRNA 분석을 통해 Pseudomonas spp.로 밝혀졌다. 현재 4개의 균주는 적정 배양조건과 제제화를 위한 연구 중에 있다.
Bacterial isolates collected from rhizosphere of turfgrass showed strong in vitro antagonistic activities against a number of turfgrass soilborne pathogens such as Rhizoctonia cerealis, R. solani AG-1(1B), Sclerotinia homoeocarpa and Typhula incarnata. In vivo study, four bacterial isolates selected...
Bacterial isolates collected from rhizosphere of turfgrass showed strong in vitro antagonistic activities against a number of turfgrass soilborne pathogens such as Rhizoctonia cerealis, R. solani AG-1(1B), Sclerotinia homoeocarpa and Typhula incarnata. In vivo study, four bacterial isolates selected have control values over 60% against one or more turfgrass pathogenic fungi. The antagonistic effects of the bacterial isolates varied depending on fungal species, host plant, and disease pressure, indicating that control effects of the antagonists could be variable depending on field conditions. They were classified as belonging to the genus Pseudomonas species, based on morphological and biochemical characteristics as well as 16S rRNA analysis. The four bacterial isolates are under a study for finding proper cultural conditions and determination formulation type.
Bacterial isolates collected from rhizosphere of turfgrass showed strong in vitro antagonistic activities against a number of turfgrass soilborne pathogens such as Rhizoctonia cerealis, R. solani AG-1(1B), Sclerotinia homoeocarpa and Typhula incarnata. In vivo study, four bacterial isolates selected have control values over 60% against one or more turfgrass pathogenic fungi. The antagonistic effects of the bacterial isolates varied depending on fungal species, host plant, and disease pressure, indicating that control effects of the antagonists could be variable depending on field conditions. They were classified as belonging to the genus Pseudomonas species, based on morphological and biochemical characteristics as well as 16S rRNA analysis. The four bacterial isolates are under a study for finding proper cultural conditions and determination formulation type.
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문제 정의
They were responsible for the antifungal activities against many plant pathogens as well as turfgrass pathogenic fungi. The objectives of thisstudy were to identify the isolates and evaluate the antifungal activity of the isolates against turfgrass pathogenic fungi.
제안 방법
The infected plants were moved to greenhouse for assessing the disease severity. After a week, disease severity was assessed by a visual estimation of the percentage of diseased areas. This experiment was conducted twice using a randomized block plot design with three replications.
The plants were grown in the growth chamber at 18 to 28℃ with light and dark cycle of 16 and 8 hours, respectively. The plants were mowed weekly with scissors to a height of 0.6 cm (creeping bentgrass) and 2cm (Kentucky bluegrass) from the beginning 2 weeks. Fertilizer (SunGrow company, Austin, Texas, USA) was applied at 0.
After a week, disease severity was assessed by a visual estimation of the percentage of diseased areas. This experiment was conducted twice using a randomized block plot design with three replications.
대상 데이터
Rhizoctonia solani AG-1(1B) (KACC-40142) was obtained from Korea Agricultural Culture Collection (KACC), Rural Development Administration, Korea. R.
The two cultivars (Penncross: 0.01g of seeds, Prosperity: 0.03g of seeds) were evenly sown into a plastic pot (5.3×5.3×5.1cm) containing commercial potting soil mixture (Metro Mix 366-P, Scott’s company, Marysville, OH, USA).
데이터처리
Analysis of variance of all data was performed and the least significant-difference (LSD, α=0.05) test was used for means separation,using PROC ANOVA (SAS Institute Inc., Cary, NC).
후속연구
Further research should focus on field evaluation to confirm the current results. Identification at the species level and knowing the mode of action of the candidates are very important for the next steps such as mass production and determination of formulation type (Schisler et al.
참고문헌 (18)
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