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논문 상세정보

Abstract

Five kinds of coumarin compounds were successfully purified from Angelica gigas Nakai by using recyclingpreparative HPLC and identified as decursin (1), decursinol angelate (2), 7-demethylsuberosine (3), marmesin (4), and decursinol (5) by NMR analyses. None of the purified compounds in ethanol showed DPPH radical scavenging activity, while the A. gigas extract (AGNEX) displayed a significant level of activity. Interestingly, compounds 3 in phosphate buffered saline (PBS) showed good $ABTS^+$ radical scavenging activity ($IC_{50}=8.1{\mu}g$/mL) as did compounds 4 and 5. The anti-inflammatory activities of the purified compounds were evaluated and compared using the NO concentration assay and western blot analysis on LPS-stimulated RAW 264.7 cells. NO production was significantly suppressed by all the compounds in a dose-dependent manner among which compounds 1, 2, and 3 showed very good activities with $IC_{50}$ values of 7.4, 6.5, and $7.6{\mu}g$/mL, respectively. Treatment with compounds 1-5 effectively suppressed the expression levels of iNOS, IL-1$\beta$, and COX-2, which are responsible for promoting the inflammatory process. Thus, the ethanol extract and coumarin compounds of A. gigas Nakai hold promise for use as potential anti-inflammatory agents.

참고문헌 (32)

  1. Sarker SD, Nahar L. 2004. Natural medicine: the genus Angelica. Curr Med Chem 11: 1479-1500 
  2. Lee S, Shin DS, Kim JS, Oh KB, Kang SS. 2003. Anti-bacterial coumarins from Angelica gigas roots. Arch Pharm Res 26: 449-452 
  3. Lee EO, Lee HJ, Lee JH, Kim MS, Kim SH, Lu J. 2006. Potent antiandrogen and androgen receptor activities of an Angelica gigas-containing herbal formulation: identification of decursin as a novel and active compound with implications for prevention and treatment of prostate cancer. Cancer Res 66: 453-463 
  4. Song GY, Lee JH, Cho M, Park BS, Kim DE, Oh S. 2007. Decursin suppresses human androgen-independent PC3 prostate cancer cell proliferation by promoting the degradation of $\beta$-catenin. Mol Pharmacol 72: 1599-1606 
  5. Lee S, Lee YS, Jung SH, Shin KH, Kim BK, Kang SS. 2003. Anti-tumor activities of decursinol angelate and decursin from Angelica gigas. Arch Pharm Res 26: 727-730 
  6. Kim JH, Jeong JH, Jeon ST, Kim H, Ock J, Suk K, Kim SI, Song KS, Lee WH. 2006. Decursin inhibits induction of inflammatory mediators by blocking nuclear factor-kB activation in macrophages. Mol Pharmacol 69:1783-1790 
  7. Kang SY, Lee KY, Sung SH, Kim YC. 2005. Four new neuroprotective dihydropyrano-coumarins from Angelica gigas. J Nat Prod 68: 56-59 
  8. Kang SY, Lee KY, Koo KA, Yoon JS, Lim SW, Kim YC, Sung SH. 2005. ESP-102, a standardized combined extract of Angelica gigas, Saururus chinensis and Schizandra chinensis, significantly improved scopolamine-induced memory impairment in mice. Life Sci 76: 1691-1705 
  9. Lee YY, Lee S, Jin JL, Yun-Choi HS. 2003. Platelet anti- aggregatory effects of coumarins from the roots of Angelica genuflexa and A. gigas. Arch Pharm Res 26: 723-726 
  10. Ahn MJ, Lee MK, Kim YC, Sung SH. 2008. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J Pharm Biomed Anal 46: 258-266 
  11. Kim MR, Abd El-Aty AM, Kim IS, Shim JH. 2006. Determination of volatile flavor components in danggui cultivars by solvent free injection and hydrodistillation followed by gas chromatographic-mass spectrometric analysis. J Chromatogr A 1116: 259-264. 
  12. Han J, Weng XC, Bi KS. 2008. Antioxidants from a Chinese medicinal herb-Litho-spermum erythrorhizon. Food Chem 106: 2-10 
  13. Fautz R, Husen B, Hechenberger C. 1991. Application of the neutral red assay to monolayer cultures of primary hepatocytes: Rapid colorimetric viability determination for the unscheduled DNA synthesis test. Mutat Tes 253: 173-179 
  14. D'Agostino P, Ferlazzo V, Milano S, LaRosa M, Di Bella G, Caruso R. 2001. Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and interleukin-12 synthesis in J774 cell line. Int Immunopharmacol 1: 1765-1776 
  15. Kim KM, Jung JY, Hwang SW, Kim MJ, Kang JS. 2009. Isolation and purification of decursin and decursinol angelate in Angelica gigas Nakai. J Korean Soc Food Sci Nutr 38: 653-656 
  16. Ryu KS, Hong ND, Kim NJ, Kong YY. 1990. Studies on the coumarin constituents of the root of Angelica gigas Nakai. Kor J Pharmacogn 21: 64-68 
  17. Nemoto T, Ohshima T, Shibasaki M. 2003. Enantioselective total syntheses of (1)-decursin and related natural compounds using catalytic asymmetric epoxidation of an enone. Tetrahedron 59: 6889-6897 
  18. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. 2002. Free radical scavenging properties of wheat extracts. J Agric Food Chem 50:1619-1624 
  19. Micking JM, Xie QW, Nathan C. 1997. Nitric oxide and macrophage function. Annu Rev Immunol 15: 323-350 
  20. Gobert AP, Mersey BD, Cheng Y, Blumberg DR, Newton JC, Wilson KT. 2002. Cutting edge: urease release by Helicobacter pylori stimulates macrophage inducible nitric oxide synthase. J Immunol 168: 6002-6006 
  21. Shen SC, Lee WR, Lin HY, Huang HC, Ko CH, Yang LL. 2002. In vitro and in vivo inhibitory activities of rutin, wogonin, and quercetin on lipopolysaccharide-induced nitric oxide and prostaglandin E(2) production. Eur J Pharmacol 446:187-194 
  22. Lee SK, Hong CH, Huh SK, Kim SS, Oh OJ, Min HY. 2002. Suppressive effect of natural sesquiterpenoids on inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) activity in mouse macrophage cells. J Environ Pathol Toxicol Oncol 21: 141-148 
  23. Ross R. 1999. Atherosclerosis-an inflammatory disease. N Engl J Med 340: 115-126 
  24. SH, Lu J. 2007. Decursin and decursinol angelate inhibit estrogen-stimulated and estrogen-independent growth and survival of breast cancer cells. Breast Cancer Res 9: R77 
  25. Choi YE, Ahn H, Ryu JH. 2000. Polyacetylenes from Angelica gigas and their inhibitory activity on nitric oxide synthesis in activated macrophages. Biol Pharm Bull 23: 884-886 
  26. Yan JJ, Kim DH, Moon YS, Jung JS, Ahn EM, Baek NI, Song DK. 2004. Protection against $\beta$-amyloid peptide-induced memory impairment with long-term administration of extract of Angelica gigas or decursinol in mice. Prog Neuropsychopharmacol Biol Psychiatry 28: 25-30 
  27. Kang SY, Lee KY, Sung SH, Park MJ, Kim YC. 2001. Coumarins isolated from Angelica gigas inhibit acetylcholinesterase: structure-activity relationships. J Nat Prod 64: 683-685 
  28. Kim MR, Abd El-Aty AM, Choi JH, Lee KB, Shim JH. 2006. Identification of volatile components in Angelica species using supercritical- $CO_2$ fluid extraction and solid phase microextraction coupled to gas chromatographymass spectrometry. Biomed Chromatogr 20: 1267-1273 
  29. Park JY, Cho HY, Kim JK, Noh KH, Yang JR, Ahn JM, Lee MO, Song YS. 2005. Chlorella dichloromethane extract ameliorates NO production and iNOS expression through the down-regulation of NFB activity mediated by suppressed oxidative stress in RAW 264.7 macrophages. Clin Chim Acta 351: 185-196 
  30. Lu YR, Yeap Foo L. 2001. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 75: 197-202 
  31. Kim DO, Lee KW, Lee HJ, Lee CY. 2002. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic Phytochemicals. J Agric Food Chem 50: 3713-3717 
  32. Lee AK, Sung SH, Kim YC, Kim SG. 2003. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, $TNF-{\alpha}$ and COX-2 expression by suchinone effects on $I-{\kappa}\;B{\alpha}$phosphorylation, C/EBP and AP-1 activation. Br J Phamacol 139: 11-20 

이 논문을 인용한 문헌 (3)

  1. 2009. "" Journal of food science and nutrition, 14(4): 269~276 
  2. Park, Yu-Hwa ; Lim, Sang-Hyun ; Kim, Hee-Yeon ; Park, Min-Hee ; Lee, Kwang-Jae ; Kim, Kyung-Hee ; Kim, Young-Guk ; Ahn, Young-Sup 2011. "Biological Activities of Extracts from Flowers of Angelica gigas Nakai" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(8): 1079~1085 
  3. 2014. "" BMB reports, 47(2): 98~103 

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