[논문]Evaluation of the Biological Activity of Extracts from Star-Anise (Illicium verum)

Chung, Hai-Jung

doi:10.3746/jfn.2009.14.3.195

Evaluation of the Biological Activity of Extracts from Star-Anise (Illicium verum) 원문보기

Journal of food science and nutrition, v.14 no.3, 2009년, pp.195 - 200

Chung, Hai-Jung (Department of Food Science and Nutrition, Daejin University)

Abstract ▼ AI-Helper

This study was carried out to investigate the biological activities of star anise extracts obtained from four different solvents (water, methanol, ethanol and chloroform) by measuring total polyphenol and flavonoid contents, DPPH radical scavenging, hydroxyl radical scavenging, nitrite scavenging activity and antimicrobial activity. The methanol extract showed the highest extraction yield, followed by ethanol, water and chloroform. The properties of the extracting solvents significantly affected the total polyphenol content. Methanol extracts contained more total polyphenols than any other extracts. The highest DPPH radical scavenging activity was found in methanol extract. The hydroxyl radical and nitrite scavenging activity were highest in methanol and ethanol extracts. In antimicrobial activity, water extract showed stronger activities than methanol and ethanol extract against Micrococcus luteus and Bacillus subtilis, and no inhibitory effects on Gram negative bacteria were found in all extracts at the concentration used.

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문제 정의

Limited research has been done on the biological activities of star anise. Therefore, the objective of this study was to investigate the total polyphenol and fla-vonoid contents of star anise, as well as the biological activities of the extracts obtained from various solvent systems having diverse polarities.

제안 방법

The nitrite scavenging activity was measured according to the method of Kato et al. (11) with modification. An aliquot (0.

대상 데이터

Star anise was purchased from Kyungdong market (Seoul, Korea) in a dried form. 1,1-Diphenyl-2-picrylhy-drazyl (DPPH) radical, trichloroacetic acid (TCA), 2-thio-barbituric acid (TBA), gallic acid, naringin, deoxyribose, ethylenediaminetetraacetic acid (EDTA) and ascorbic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Folin–Ciocalteu were purchased from Fluka (Switzerland).

데이터처리

1)Means with different letters within a column are significantly different from each other at p<0.05 as determined by Duncan's multiple range test.
1)Means with different letters within a row are significantly different from each other at p<0.05 as determined by Duncan's multiple range test.
2)Means with different letters within a row are significantly different from each other at p<0.05 as determined by Duncan's multiple range test.

이론/모형

The microorganisms were grown overnight at 37℃ in nutrient broth. Antimicrobial activity of extract samples were investigated by the disc diffusion method. Forty μL (100 mg/mL concentration) of each extracts were impregnated on sterile paper discs of 8 mm diameter and discs were let to dry to remove any residual solvent which might interfere with the result.
The DPPH radical scavenging activity of star anise was measured according to the method of Blois (9) with modification. An aliquot (0.
The standard curve for total polyphenol content was made using gallic acid standard solution and total poly- phenolics were expressed as mg of gallic acid equivalents (GAE)/g star anise extract. The flavonoid content was determined by using a colorimetric method described by Lee and Kim (8) with slight modification. An aliquot (0.
The hydroxyl radical scavenging activity of star anise extract was measured according to the method of Nagai et al. (10) with modification. The reacting mixture, which contained 100 μL of a premixed 10 mM FeSO₄ and 10 mM EDTA (1:1, v/v), 200 μL of 10 mM deoxyribose, 1.
Total polyphenol content was determined using a modified version of the Folin-Ciocalteu method (7). An aliquot (0.

성능/효과

2 suggests that nitrosamine production can be inhibited in vivo. From these findings, it was sug- gested that methanol or ethanol extracts of star anise would be an effective nitrite scavenger at pH values low- er than 3.0.
1. Water extract was most effective to inhibitory to Micrococcus luteus and Bacillus subtilis, showing the average zone of inhibition of 27.5 and 18.5 mm, respectively. Methanol extract exhibited the zone of in- hibition 22.

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