Objective: This study was to investigate the anti-tumor effect, safety, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB (APL) in female C57B/L mouse tumor (in vivo). Method: First, to evaluate the antitumor activity of APL, we divided the mice into four groups: normal, control, APL50 (50mg/kg), and APL100 (100mg/kg). LLC-obtained American Type Culture Collection was used. LLC had been inoculated to induce tumors. To measure the anti-tumor effect of APL, we calibrated tumor size and weight. To analyze the mechanism of anti-tumor in APL, we used western blotting and to observe metabolizing enzyme in APL we used to real-time PCR. Result: APL50 and APL100 significantly inhibited tumor growth from 12 days after medicine injected. APL did not induce caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it decreased 41% and 71% in CYP2D22 and CYP3A11, respectively. Conclusion: These results suggest that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be used carefully with other drugs related with CYP2D22 and CYP3A11.
Sung HS. Anti-cancer Effects of Kamiboa-tang and some other Traditional Medical Prescriptions. Korean J. Orient.Int. Med. 2007;28(2):321-32.
Park JS, Oh YJ, Jang HJ, Choi YH, Park EA. An Analysis of Nursing Research on Cancer Prevention and Early Detection, Reported in Korea from 1980-2001. The J. of Korean Community Nursing. 2002;13(2):363-75.
Cao LH, Lee JK, Cho KH, Kang DG, Kwon TO, Kwon JW, et al.Mechanism for the Vascular Relaxation Induced by Butanol Extract of Agrimonia pilosa. Korean Journal of Pharmacognosy. 2006;37(2):67-73.
Kloft C, Wallin J, Henningsson A, Chatelut E, Karlsson MO. Population pharmacokinetic-pharmacodynamic model for neutropenia with patient subgroup identification: comparison across anticancer drugs. Clin Cancer Res. 2006;12(18):5481-90.
Green DR. Apoptotic pathways: paper wraps stone blunts scissors. Cell. 2000;102;1-4.
Van der Kolk DM, Vellenga E, Müller M, de Vries EG. Multidrug resistance protein MRP1, glutathione, and related enzymes. Their importance in acute myeloid leukemia. Adv Exp Med Biol. 1999;457:187-98. Review.
Down MJ, Arkle S, Mills JJ. Regulation and induction of CYP3A11, CYP3A13 and CYP3A25 in C57BL/6J mouse liver. Arch Biochem Biophys. 2007;457(1):105-10.
Chun SB, Yang B, Choi CW, Kim IS, Park KS. Antibacterial Activities against Plant Pathogens and Identification of Agrimol B from Agrimonia pilosa LEDEB. The Korean Journal of Pesticide Science. 2006;10(3):230-6.
Yu AM, Haining RL. Expression, purification, and characterization of mouse CYP2d22. Drug Metab Dispos. 2006;34(7):1167-74.
Gao K, Zhou L, Chen J, Li F, Zhang L. Experimental study on decoctum Agrimonia pilosa Ledeb-Ledebinduced apoptosis in HL-60 cells in vitro. Zhong Yao Cai. 2000 Sep;23(9):561-2.
Sakurai M, Ito M, Hanawa Y, Tsukimoto I, Imashuku S, Ueda K, et al. Clinical study of recombinant human granulocyte-colony stimulating factor (KW-2228) in pediatric field. 2. Effectiveness on neutropenia associated with administration of anticancer agent and safety. Rinsho Ketsueki. 1993;34(2):119-27.
Lim SS, Lee YS, Cho HJ, Shin HK, Yoon JH. Synthesis of Methoxylated Flavone Derivatives and Examination of Their Effects on HT-29 Human Colon Cancer Cell Growth. Cancer Prevention Research. Cancer Prevention Research. 2006;11:211-7.
Blume N, Leonard J, Xu ZJ, Watanabe O, Remotti H, Fishman J. Characterization of Cyp2d22, a novel cytochrome P450 expressed in mouse mammary cells. Arch Biochem Biophys. 2000;381(2):191-204.
Huttunen KM, Mähönen N, Raunio H, Rautio J. Cytochrome P450-activated prodrugs: targeted drug delivery. Curr Med Chem. 2008;15(23):2346-65.
Martignoni M, Groothuis GM, de Kanter R. Species differences between mouse, rat, dog, monkey and human cytochrome P450-mediated drug metabolism, inhibition and induction. Expert Opin Drug Metab Toxicol. 2006;2(6):875-94.
Jun SM, Ko SG, Ko HY, Choi YK, Kim DW, Park JH, Jun CY. Effects of Astragali Radix on Neutropenia Caused by Cyclophosphamide . Korean J. Orient.Int. Med. 2007;28(1):1-11.
Martinou JC, Green DR. Breaking the mitochondrial barrior. Nat Rev Mol Cell Biol. 2001;1:63-7.
Russel FG, Koenderink JB, Masereeuw R. Multidrug resistance protein 4 (MRP4/ABCC4): a versatile efflux transporter for drugs and signalling molecules. Trends Pharmacol Sci. 2008;29(4):200-7.
Miyamoto K, Kishi N, Koshiura R. Antitumor activity of methanol extract from roots of Agrimonia pilosa Ledeb. Jpn J Pharmacol. 1985 May;38(1):9-16.
Steller H. Mechanism and genes of cells suicide. Science. 1995;267:1145-9.
Ishikawa M, Okada Y, Satake-Ishikawa R, Kakitani M, Kawagishi M, Matsuki S, et al. Pharmacological effects of recombinant human granulocyte colony-stimulating factor modified by polyethylene glycol on anticancer drug-induced neutropenia in mice. Gen Pharmacol. 1994 May;25(3):533-7.
Park S, Kwon OJ. Combined Effect of Agrimonia pilosa Ledebour Extract and NaCl for Control of Escherichia coli O157:H7. Korean Journal of Biotechnology and Bioengineering. 1998;13(2):168-73.
Kim HJ, Lim HW, Kim BH, Kim HS, Choi SW, Yoon CS. Studies on the Anti-acne Effect of Agrimonia pilosa Ledeb. Journal of the Society of Cosmetic Scientists of Korea. 2006;32(1):53-8.
Meier P, Finch A. and Evan, G. Apoptosis in development. Nature. 2000;407:796-801
Keppler D, Leier I, Jedlitschky G, König J. ATP-dependent transport of glutathione S-conjugates by the multidrug resistance protein MRP1 and its apical isoform MRP2. Chem Biol Interact. 1998;111-112:153-61.
Lee YH, Kim MB, Chung DS. Effect of Extract Agrimonsa Pilosa L. on blological Activity in Rats. Korean J. Medicinal Crop Sci. 2002;10(3):167-70.
Nunez G., Benedict MA, Hu Y, Inohara N. Caspases: The proteases of the apoptotic pathway. Oncogene. 1998;17:3237-45.
Deeley RG, Cole SP. Substrate recognition and transport by multidrug resistance protein 1 (ABCC1). FEBS Lett. 2006;580(4):1103-11.
Kang SC et al. Hepatoprotective Effects of Aqueous Extract from Aerial Part of Agrimmony. Kor. J. Parmacogn. 2006;37(1):28-32.
Kothakota S, Azuma T, Reinhard C, et al. Caspase-3-generated fragment of gelsolin: Effector of morphological change in apoptosis. Science. 1997;278:294-8.
Shin MK, Kim BS, Oh JH, Lim HY, Kim DW, Choi BH, Byun JS. The Anti-tumor Effects of Soonkiwhajungtang with Doxorubicin in Colon-26. Korean J. Orient.Int. Med. 2004;25(2):183-94.
Murayama T, Kishi N, Koshiura R, Takagi K, Furukawa T, Miyamoto K. Agrimoniin, an antitumor tannin of Agrimonia pilosa Ledeb., induces interleukin-1. Anticancer Res. 1992 Sep-Oct;12(5):1471-4.
Jonstone RW. Apoptosis: a link between cancer genetics and chemotherapy. Cell. 2002;108:153-64.
Yang JC, Chung WK. Psychology and Quality of Life in Cancer Patients on Radiation Therapy. The J. of Korean Society for Therapeutic Radiology & Oncology. 2004;22(4):271-9.
이 논문을 인용한 문헌 (1)
Ryu, Hee Kyoung ; Goo, Bon Hyuk ; Suk, Kyung Hwan ; Lee, Ju Hyeon ; Ryu, Soo Hyeong ; Lee, Su Yeon ; Kim, Min Jeong ; Park, Yeon Cheol ; Baek, Yong Hyeon ; Park, Dong Suk ; Seo, Byung Kwan 2014. "The Current Research Methodology of Pharmacopucture for the Treatment of Animal Cancer Models in Korea" The acupuncture = 대한침구의학회지, 31(4): 81~97