$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

초록

잎녹차 추출물 보다는 분말녹차 추출물의 경우가 그리고 저장된 sample보다는 fresh sample의 AChE 저해활성이 상대적으로 높았고 이는 생리활성 물질의 추출효율성과 생리활성 물질의 특성 변화 측면에서 기인되는 것으로 판단된다. 또한 본 연구팀에서 구성한 저장 조건에서, 4주간의 짧은 저장기간이 3개월 저장조건보다는 우수한 효소 저해활성도를 나타냈다. 수분활성도 측면에서는 수분활성도 0.81의 조건을 갖는 sample에서의 효과가 비교적 우수했고, 3개월 저장하는 동안의 저장온도 조건은 효소 저해 활성도에 큰 차이를 보이는 요소로 작용하지 못했다. 다만 상온이나 냉장조건 보다는 냉동상태의 저장조건이 다소 우수한 것으로 판단된다. 결국 생리활성물질을 보다 효율적으로 추출할 수 있는 녹차의 상태와 처리 또는 섭취 농도가 더욱 중요한 것으로 사료되고, 추출 효율을 증가시키기 위해서는 잎 보다는 분말의 상태가 더 우수한 것으로 판단된다. 따라서 녹차 추출물은 acetylcholine성 신경세포의 기능을 강화시켜줄 수 있는 AChE 저해제로서 활용가치가 높을 것이다.

Abstract

Acetylcholinesterase (AChE) inhibitors, which enhance cholinergic transmission by reducing the enzymatic degradation of acetylcholine, are the only source of the compound that is currently approved for the treatment of Alzheimer's disease (AD). In these experiments, the concentration samples of green tea extracts were 100, 500, and 1,000 ${\mu}g$/mL. Among them, the highest concentration (1,000 ${\mu}g$/mL) of fresh green tea extracts showed the most potent inhibitory effect on AChE by reducing more than 40% of enzyme activity, and in a concentration-dependent pattern. In addition, we examined the effect of other storing conditions on AChE inhibition. In order to maintain storage for 3 months, the materials were held at the certain storing conditions of temperature (room temperature, 4 and $-20^{\circ}C$) and for water activity (0.81, 0.69, and 0.23). In these storing conditions, the difference in temperature did not contribute to the AChE inhibitory effect. Our presented data showed that the AChE inhibitory effect was affected by the concentration of green tea extract and by water activity (0.81). These results suggest that green tea may serve as a potential dietary source of AChE inhibitor.

참고문헌 (30)

  1. Harada J, Sugimoto M. Activation of caspase-3 in $\beta$-amyloidinduced apoptosis of cultured rat cortical neurons. Brain Res. 842: 311-323 (2000) 
  2. Goodman Y, Mattson MP. Secreted forms of $\beta$-amyloid precursor protein protect hippocampal neurons against amyloid β-peptideinduced oxidative injury. Exp. Neurol. 128: 1-12 (1994) 
  3. Polinsky RJ. Clinical pharmacology of rivastigmine: A new generation acetylcholinesterase inhibitor for the treatment of Alzheimer"s disease. Clin. Ther. 20: 634-47 (1998) 
  4. Shimizu M, Deguchi A, Lim JT, Moriwaki H, Kopelovich L, Weinstein IB. (-)-Epigallocatechin gallate and polyphenon Einhibit growth and activation of the epidermal growth factor receptor and human epidermal growth factor receptor-2 signaling pathways in human colon cancer cells. Clin. Cancer Res. 11: 2735-2746 (2005) 
  5. Youn KS. Absorption characteristics of green tea powder as influenced by particle size. J. Korean Soc. Food Sci. Nutr. 33: 1720-1725 (2004) 
  6. Choi WH, Um MY, Ahn JY, Kim SR, Kang MH, Ha TY. Acetylcholinesterase inhibitory activity and protective effect against cytotoxicity of perilla seed methanol extract. Korean J. Food Sci. Technol. 36: 1026-1031 (2004) 
  7. Kim YI, Park JY, Choi SJ, Kim JK, Jeong CH, Choi SG, Lee SC, Cho SH, Heo HJ. Protective effect of green tea extracts on amyloid $\beta$ peptide-induced neurotoxicity. Korean J. Food Preserv. 15: 743-748 (2008) 
  8. Koh SH, Kim SH, Kwon H, Kim JG, Kim JH, Yang KH, Kim J, Kim SU, Yu HJ, Do BR, Kim KS, Jung HK. Phosphatidylinositol-3 kinase/Akt and GSK-3 mediated cytoprotective effect of epigallocatechin gallate on oxidative stress-injured neuronaldifferentiated N18D3 cells. Neurotoxicology 25: 793- 802 (2004) 
  9. Youn KS. Absorption characteristics of green tea powder as influenced by particle size. J. Korean Soc. Food Sci. Nutr. 33: 1720-1725 (2004) 
  10. Behl C, Davis JB, Cole GM, Schubert D. Vitamin E protects nerve cells from amyloid $\beta$ protein toxicity. Biochem. Bioph. Res. Co. 186: 944-950 (1992) 
  11. Schroder H, Giacobini E, Struble RG, Luiten PG, Zilles K, Strosberg AD. Muscarinic cholinoceptive neurons in the frontal cortex in Alzheimer's disease. Brain Res. Bull. 27: 631-636 (1991) 
  12. Webster RA, Jordan CC. Neurotransmitters, Drugs and Disease. Blacewell Scienific Publication. London, England. pp. 428-445 (1989) 
  13. Aarsland D, Larsen JP, Reinvamg I, Aarsland AM. Effect of cholinergic blockade on language in healthy young women. Brain 117: 1377-1384 (1994) 
  14. Bowen DM, Benton JS, Spillane JA, Smith CC, Allen SJ. Choline acetyltransferase activity and histopathology of frontal neocortex from biopsies of demented patients. J. Neurol. Sci. 57: 191-2024 (1982) 
  15. Masuda M, Suzui M, Weinstein IB. Effects of epigallocatechin-3-gallate on growth, epidermal growthfactor receptor signaling pathways, gene expression, and chemosensitivity in human head and neck squamous cell carcinoma cell lines. Clin. Cancer Res. 7: 4220-4229 (2001) 
  16. Yan XZ, Qiao JT, Dou Y, Qiao ZD. Beta-amyloid peptide fragment 31-35 induces apoptosis in cultured cortical neurons. Neuroscience 92: 177-184 (1999) 
  17. Jeong DH, Kim JT. The Science of Tea. Daegwang Press, Seoul, Korea. pp. 87-92 (1997) 
  18. Park JH, Back CN, Kim JK. Recommendation of packing method to delay the quality decline of green tea powder stored at room temperature. Korean J. Hort. Sci. Technol. 23: 499-506 (2005) 
  19. Ko WJ, Ko KS, Kim YD, Jeong KW, Lee SH, Koh JS. Changes in functional constituents and stability of green tea beverage during different storing conditions. Korean J. Food Preserv. 13: 421-426 (2006) 
  20. Selkoe DJ. The molecular pathology of Alzheimer's disease. Neuron 6: 487-498 (1991) 
  21. Hachiski VC, Illiff LD, Zikhka, E, Du boulay GH, McAllister VL, Russel RW, Symon L. Crebral blood flow in dementia. Arch. Neurol. 32: 632-637 (1975) 
  22. Kim MK, Jung HS, Yoon CS, Kwon MJ, Koh KS, Rhee BD, Park JH. The protective effect of EGCG on INS-1 cell in the oxidative stress and mechanism. Korean Diabetes J. 32: 121-131 (2008) 
  23. Maeda S, Nakagawa M. General chemical and physical analysis on various Kinds of green tea. Chagyo Kenkyuhokoku 45: 85-92 (1977) 
  24. Perry EK, Perry RH, Blessed G, Tomlinson, BE. Necropsy evidence of central cholinergic deficts in senile dementia. Lancet 1: 8004-8189 (1997) 
  25. Park JH, Kim YO, Kug YI, Cho DB, Choi HK. Effects of green tea powder on noodle properties. J. Korean Soc. Food Sci. Nutr. 32: 1021-1025 (2003) 
  26. Chen ZP, Schell JB, Ho CT, Chen KY. Green tea epigallocatechin gallate shows a pronounced growth inhibitory effect on cancerous cells but not on their normal counterparts. Cancer Lett. 129: 173-179 (1998) 
  27. Lee JM, Lim SW, Cho SH, Choi SG, Heo HJ, Lee SC. Effect of relative humidity and storage temperature on the quality of green tea powder. J. Korean Soc. Food Sci. Nutr. 38: 83-88 (2009) 
  28. Hock C, Konietzko U, Streffer JR, Tracy J, Signorell A, Muller- Tillmanns B, Lemke U, Henke K, Moritz E, Garcia E, Wollmer MA, Umbricht D, Quervain D JF, Hofmann M, Maddalena A, Papassotiroplos A, Nitsch RM. Antibodies against $\beta$-amyloid slow cognitive decline Alzheimer's disease. Neuron 38: 547-554 (2003) 
  29. Choi JH, Kim DW, Choi JS, Han YS, Baek YH. Effect of reed root extract (RRE) on learning and memory impairment animal model SAMP8 strain. Ⅲ. Feeding effect of RRE on neurotransmitters and their metabolites in SAMP8 brain. Korean J. Genet. 7: 29-36 (1997) 
  30. Jang CH, Eun JS, Park HW, Seo SM, Yang JH, Leem KH, Oh SH, Oh CH, Baek NI, Kim DK. An acetylcholinesterase inhibitor from the leaves of securinega suffruticosa. Korean J. Pharmacogn. 34: 14-17 (2003) 

이 논문을 인용한 문헌 (4)

  1. Seo, Dong-Soo ; Jang, Jeong-Hoon ; Kim, Na-Mi ; Lee, Jong-Soo 2009. "Optimal Extraction Condition and Characterization of Antidementia Acetylcholinesterase Inhibitor from Job's Tears (Coix lachrymajobi L.)" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 17(6): 434~438 
  2. Lee, Eun-Na ; Song, Jung-Hwa ; Lee, Jong-Soo 2010. "Screening of a Potent Antidementia Acetylcholinesterase Inhibitor-containing Fruits and Optimal Extraction Conditions" 한국식품영양학회지 = The Korean journal of food and nutrition, 23(3): 318~323 
  3. 2010. "" Mycobiology, 38(1): 58~61 
  4. 2011. "" Nutrition research and practice, 5(4): 294~300 

DOI 인용 스타일