$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Glutamate receptor-interacting protein 1 단백질과 armadillo family 단백질 p0071/plakophilin-4와의 결합

Glutamate Receptor-interacting Protein 1 Protein Binds to the Armadillo Family Protein p0071/plakophilin-4 in Brain

생명과학회지 = Journal of life science, v.19 no.8 = no.112, 2009년, pp.1055 - 1061  

문일수 (동국대학교 의과대학 해부학교실) ,  석대현 (인제대학교 의과대학생화학교실)

초록
AI-Helper 아이콘AI-Helper

${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) 수용체중추신경계에 널리 발현하며, 연접부위에서 글루타메이트에 의한 흥분성 신경전달의 역할을 행한다. 이러한 수용체의 조절은 학습과 기억에 관여한다. 본 연구에서는 AMPA receptor subunit glutamate receptor-interacting protein 1 (GRIP1)과 결합하는 단백질로 armadillo family인 p0071/plakophilin-4을 분리하였다. GRIPl 단백질은 p0071/plakophilin-4 단백질의 C-말단과 결합하며, armadillo family 단백질 중 p0071/plakophilin-4 과만 결합한다. 효모 two-hybrid assay에서 GRIPl의 Postsynaptic density-95/Discs large/Zona occludens-1 (PDZ) 영역이 p0071/plakophilin-4 단백질과의 결합에 관여하며, p0071/plakophilin-4 단백질의 C-말단 아미노산인 "S-X-V" 배열이 결합에 필수적이었다. 이러한 결합은 뇌 균질액에서 Glutathione S-transferase (GST) pull-down assay와 공면역침강법으로 확인하였다. 이러한 결과들은 AMPA 수용체가 연접후막에 안정적으로 위치하는 새로운 역할을 시사한다.

Abstract AI-Helper 아이콘AI-Helper

${\alpha}$-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors are widespread throughout the central nervous system and appear to serve as synaptic receptors for fast excitatory synaptic transmission mediated by glutamate. Their modulation is believed to affect learning and m...

주제어

AI 본문요약
AI-Helper 아이콘 AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

  • To address the assembling and localization of the GRIP1/AMPA receptor complex, it was necessary to identify the GRIPl-interacting proteins. In this study yeast two-hy­ brid selection was performed, in which the PDZ domains 1, 2, and 3 of GRIP1 were used as baits. A single interacting protein was discovered, termed p0071/plakophilin-4, an ar­ madillo repeat protein linked to the cytoskeleton.

대상 데이터

  • These clones were individually isolated, sequenced and sub­ jected to further yeast two-hybrid filter assay to confirm the interactions. Among these, ten clones were identified as p0071/plakophilin-4 and overlapped at the C-terminal re­ gion (Fig. 1A). p0071/plakophilin4 is a member of the arma­ dillo protein family (Fig.
  • The filled box corresponds to armadillo repeat domain and the open box to the coiled-coil domain. Selected clones were overlapped at the open reading frame (ORF) of p0071/plakophilin-4. aa, the amino acid residue number.
  • (RT-PCR). The amplified fragment was sub-cloned into pLexA and pB42AD (Qontech, Palo Alto, CA, USA). The correct orientation of the cDNA inserts was verified by restriction enzyme analysis, and sequence analysis was used to check that they were in-frame.

이론/모형

  • Library inserts analyzed by restriction enzyme digestion. Unique inserts were sequenced, and DNA and protein se­ quence analysis were performed with the BLAST algorithm at the National Center for Biotechnology Information (NCBI). After isolation of the plasmids encoding the library clones, these plasmids were tested for interactions of the re­ porter gene in yeast by the retransformation.
본문요약 정보가 도움이 되었나요?

참고문헌 (34)

  1. Bladt, F., A. Tafuri, S. Gelkop, L. Langille, and T. Pawson. 2002. Epidermolysis bullosa and embryonic lethality in mice lacking the multi-PDZ domain protein GRIP1. Proc. Natl. Acad. Sci. USA 99, 6816-6821 

  2. Bonne, S., B. Gilbert. M. Hatzfeld, X. Chen, K. J. Green, and F. van Roy. 2003. Defining desmosomal plakophilin-3 interactions. J. Cell Biol. 161, 403-416 

  3. Brenman, J. E., D. S. Chao, S. H. Gee, A. W. McGee, S. E. Craven, D. R. Santillano, Z. Wu, F. Huang, H. Xia, M. F. Peters, S. C. Froehner, and D. S. Bredt. 1996. Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains. Cell 84, 757-767 

  4. Brenman, J. E., J. R. Topinka, E. C. Cooper, A. W. McGee, J. Rosen, T. Milroy, H. J. Ralston, and D. S. Bredt. 1998. Localization of postsynaptic density-93 to dendritic microtubulesand interaction with microtubule-associated protein 1A. J. Neurosci. 18, 8805-8813 

  5. Bruckner, K., J. Pablo Labrador, P. Scheiffele, A. Herb, P. H. Seeburg, and R. Klein. 1999. EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains. Neuron 22, 511-524 

  6. Calkins, C. C., B. L. Hoepner, C. M. Law, M. R. Novak, S. V. Setzer, M. Hatzfeld, and A. P. Kowalczyk. 2003. The Armadillo family protein p0071 is a VE-cadherin- and desmoplakin- binding protein. J. Biol. Chem. 278, 1774-1783 

  7. Chen, L., S. Bao, X. Qiao, and R. F. Thompson. 1999. Impaired cerebellar synapse maturation in waggler, a mutant mouse with a disrupted neuronal calcium channel gamma subunit. Proc. Natl. Acad. Sci. USA 96, 12132-12137 

  8. Deguchi, M., T. Iizuka, Y. Hata, W. Nishimura, K. Hirao, I. Yao, H. Kawabe, and Y. Takai. 2000. PAPIN. A novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/delta-catenin and p0071. J. Biol. Chem. 275, 29875-29880 

  9. Dong, H., R. J. O'Brien, E. T. Fung, A. A. Lanahan, P. F. Worley, and R. L. Huganir. 1997. GRIP: a synaptic PDZ domain- containing protein that interacts with AMPA receptors. Nature 386, 279-284 

  10. Hashimoto, K., M. Fukaya, X. Qiao, K. Sakimura, M. Watanabe, and M. Kano. 1999. Impairment of AMPA receptor function in cerebellar granule cells of ataxic mutant mouse stargazer. J. Neurosci. 19, 6027-6036 

  11. Hatzfeld, M., C. Haffner, K. Schulze, and U. Vinzens. 2000. The function of plakophilin 1 in desmosome assembly and actin filament organization. J. Cell Biol. 149, 209-222 

  12. Hatzfeld, M. and C. Nachtsheim. 1996. Cloning and characterization of a new armadillo family member, p0071, associated with the junctional plaque: evidence for a subfamily of closely related proteins. J. Cell Sci. 109, 2767-2778 

  13. Hatzfeld, M. 2007. Plakophilins: Multifunctional proteins or just regulators of desmosomal adhesion? Biochim. Biophys. Acta. 1773, 69-77 

  14. Hatzfeld, M. 1999. The armadillo family of structural proteins. Int. Rev. Cytol. 186, 179-224 

  15. Hillier, B. J., K. S. Christopherson, K. E. Prehoda, D. S. Bredt, and W. A. Lim. 1999. Unexpected modes of PDZ domain scaffolding revealed by structure of nNOS-syntrophin complex. Science 284, 812-815 

  16. Hollmann, M. and S. Heinemann. 1994. Cloned glutamate receptors. Annu. Rev. Neurosci. 17, 31-108 

  17. Ikeda, A., Q. Y. Zheng, A. R. Zuberi, K. R. Johnson, J. K. Naggert ,and P. M. Nishina. 2002. Microtubule-associated protein 1A is a modifier of tubby hearing (moth1). Nat. Genet. 30, 401-405 

  18. Jaulin-Bastard, F., J. P. Arsanto, A. Le Bivic, C. Navarro, F. Vuly, H. Saito, S. Marchetto, M. Hatzfeld, M. J. Santoni, D. Birnbaum, and J. P. Borg. 2002. Interaction between Erbin and a Catenin-related protein in epithelial cells. J. Biol. Chem. 277, 2869-2875 

  19. Kim, S. J., C. H. Lee, H. Y. Park, S. S.Yea, W. H. Jang, S. K. Lee, Y. H. Park, O. S. Cha, I. S. Moon, and D. H. Seog. 2007. JSAP1 interacts with kinesin light chain 1 through conserved binding segments. J. Life Sci. 17, 889-895 

  20. Lampugnani, M. G., M. Corada, L. Caveda, F. Breviario, O. Ayalon, B. Geiger, and E. Dejana. 1995. The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin 

  21. Leonard, A. S., M. A. Davare, M. C. Horne, C. C. Garner, and J. W. Hell. 1998. SAP97 is associated with the alpha- amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor GluR1 subunit. J. Biol. Chem. 273, 19518-19524 

  22. Lin, D., G. D. Gish, Z. Songyang, and T. Pawson. 1999. The carboxyl terminus of B class ephrins constitutes a PDZ domain binding motif. J. Biol. Chem. 274, 3726-3733 

  23. Mertens, C., C. Kuhn, and W. W. Franke. 1996. Plakophilins 2a and 2b: constitutive proteins of dual location in the karyoplasm and the desmosomal plaque. J. Cell Biol. 135, 1009-1025 

  24. Okada, Y., H. Yamazaki, Y. Sekine-Aizawa, and N. Hirokawa. 1995. The neuron-specific kinesin superfamily protein KIF1A is a unique monomeric motor for anterograde axonal transport of synaptic vesicle precursors. Cell 81, 769-780 

  25. Osten, P., L. Khatri, J. L. Perez, G. Kohr, G. Giese, C. Daly, T. W. Schulz, A. Wensky, L. M. Lee, and E. B. Ziff. 2000. Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor. Neuron 27, 313-325 

  26. Setou, M., D. H. Seog, Y. Tanaka, Y. Kanai, Y. Takei, M. Kawagishi, and N. Hirokawa. 2002. Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites. Nature 417, 83-87 

  27. Sheng, M. and C. Sala. 2001. PDZ domains and the organization of supramolecular complexes. Annu. Rev. Neurosci. 24, 1-29 

  28. Sheng, M. 2001. Molecular organization of the postsynaptic specialization. Proc. Natl. Acad. Sci. USA 98, 7058-7061 

  29. Song, I. and R. L. Huganir. 2002. Regulation of AMPA receptors during synaptic plasticity. Trends Neurosci. 25, 578-588 

  30. Srivastava, S. and E. B. Ziff. 1999. ABP: a novel AMPA receptor binding protein. Ann. N Y Acad. Sci. 86, 561-564 

  31. Stahl, B., A. Diehlmann, and T. C. Sudhof. 1999. Direct interaction of Alzheimer's disease-related presenilin 1 with armadillo protein p0071. J. Biol. Chem. 274, 9141-9148 

  32. Torres, R., B. L. Firestein, H. Dong, J. Staudinger, E. N. Olson, R. L. Huganir, D. S. Bredt, N. W. Gale, and G. D. Yancopoulos. 1998. PDZ proteins bind, cluster, and synaptically colocalize with Eph receptors and their ephrin ligands. Neuron 21, 1453-1463 

  33. Xia, J., X. Zhang, J. Staudinger, and R. L. Huganir. 1999. Clustering of AMPA receptors by the synaptic PDZ domain-containing protein PICK1. Neuron 22, 179-187 

  34. Ye, B., D. Liao, X. Zhang, P. Zhang, H. Dong, and R. L. Huganir. 2000. GRASP-1: a neuronal RasGEF associated with the AMPA receptor/GRIP complex. Neuron 26, 603-617 

저자의 다른 논문 :

관련 콘텐츠

오픈액세스(OA) 유형

BRONZE

출판사/학술단체 등이 한시적으로 특별한 프로모션 또는 일정기간 경과 후 접근을 허용하여, 출판사/학술단체 등의 사이트에서 이용 가능한 논문

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트