• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


To enhance the sensitivity of an ELISA developed for the acaricide/insecticide fenazaquin, the length of the spacer arm attached to the 7-position of the quinazoline ring in the hapten (H-I) used previously as an immunogen was shortened, and this modified hapten (H-IV) was then used as an immunogen. The new immunogen used to raise polyclonal antibodies was designated 3-(4-(4-tert-butylbenzyloxy)quinazolin-7-ylcarbamoyl)propanoic acid. Under optimized conditions of a competitive indirect ELISA (ciELISA), the former $IC_{50}$ value of 96.97 ng/mL was reduced to 42.13 ng/mL, and the detection range was 10.96-161.91 ng/mL, the lowest detection limit being 1.8 ng/mL. HPLC was run to compare with and to validate the ELISA with fortified fruit and water samples; the correlation was 0.997-1.000 (n=4-5) with a slope of 0.905-1.112. The recoveries of fenazaquin from fortified samples by the ELISA ranged from 90.61 to 115.12%, which is acceptable as an ELISA for residue analysis.

참고문헌 (17)

  1. Lee JK, Ahn KC, Stoutamire DW, Gee SJ, and Hammock BD (2003) Development of an enzyme-linked immunosorbent assay for the detection of the organophosphorus insecticide acephate. J Agric Food Chem 51, 3695-3703 
  2. Longhurst C, Bacci L, Buendia J, Hatton CJ, Petitprez J, and Tsakonas P (1992) Fenazaquin, a novel acaricide for the management of spider mites in a variety of crops. Brighton Crop Prot Conf Pests Dis 2, 51 
  3. Sugawara Y, Gee SJ, Sanborn JR, Gilman SD, and Hammock BD (1998) Development of a highly sensitive enzyme-linked immunosorbent assay based on polyclonal antibodies for the detection of polychlorinated dibenzo-p-dioxins. Anal Chem 70, 1092-1099 
  4. Kumar V, Tewary DK, Ravindranath SD, Shanker A. (2004) Investigation in tea on fate of fenazaquin residue and its transfer in brew. Food and Chemical Toxicology 42, 423-428 
  5. Harrison RO, Braun AL, Gee SJ, O'Brien DJ, and Hammock BD. (1989) Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the direct analysis of molinate(Ordram) in rice field water. Agric Food Immunol 1, 37-51 
  6. Lee JK, Kim YJ, Lee EY, Kim DK, and Kyung KS (2005) Development of an ELISA for the detection of fenazaquin residues in fruits. Agric Chem Biotechnol 48, 16-25 
  7. Langone JJ and Van Vunakis H (1975) Radioimmunoassay for dieldrin and aldrin. Res Commun Chem Pathol Pharmaco 10, 163-171 
  8. Abad A and Montoya A (1997) Development of an enzyme-linked immunosorbent assay to carbaryl. 2. Assay optimization and application to the analysis of water samples. J Agric Food Chem 45, 1495-1501 
  9. Hackler RE, Suhr RG, Sheets JJ, Hatton CJ, Johnson PL, Davis LN, Edie RG, Kaster SV, Jourdan GP, Jackson JL, and Krumkalns EV (1994) Chemistry and miticidal activity of fused pyrimidine derivatives of fenazaquin. In Advances in the Chemistry of Insect Control III, Briggs GG (ed.), pp. 70-84. Royal Soc Chem, Cambridge, UK. 
  10. Lee JK, Ahn KC, Park OS, Ko YK, and Kim DW (2002) Development of an immunoassay for the residues of the herbicide bensulfuron-methyl. J Agric Food Chem 50, 1791-1803 
  11. Lee JK, Kyung KS, Kwon JW, Jung IS, and Ahn KC (1993) Degradation of the acaricide fenazaquin in soil. In Plant Growth and Environment, p. 212. Proc Symp Korean Agric Chem Soc, Seoul, Korea 
  12. Vanderlaan M, Stanker LH, and Watkins BE (1988) Improvement and application of an immunoassay for screening environmental samples for dioxin contamination. Environ Toxicol Chem 7, 859-870 
  13. Voller A, Bidwell DE, and Bartlett A (1976) Enzyme immunoassay in diagnostic medicine: theory and practice. Bull WHO 53, 55-65 
  14. Lee JK, Park SH, Lee EY, Kim YJ, and Kyung KS (2004) Development of an ELISA for the detection of the residues of the fungicide iprovalicarb. J Agric Food Chem 52, 6680-6686 
  15. Shan G, Stoutamire DW, Wengatz I, Gee SJ, and Hammock BD (1999) Development of an immunoassay for the pyrethroid insecticide esfenvalerate. J Agric Food Chem 47, 2145-2155 
  16. Gee S J, Hammock BD, and Van Emon JM (1994) In A User's Guide to Environmental Immunochemical Analysis; EPA/540/R-94/509; U.S. EPA. Office of Research and Development, Environmental Monitoring Systems Laboratory, Las Vegas, NV, U.S.A. 
  17. Rodbard D (1981) Mathematics and statistics of ligand assay: an illustrated guide. In Ligand Assay: Analysis of International Developments on Isotopic and Nonisotopic Immunoassay, Langan J and Clapp JJ (eds.), pp.45-99. Masson Publishing, New York, NY, U.S.A. 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음


원문 PDF 다운로드

  • 원문 PDF 정보가 존재하지 않습니다.

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일