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논문 상세정보

S-Allyl Cysteine(SAC)이 제대혈 유래 중간엽 줄기세포 증식에 미치는 영향

Effect of S-Allyl Cysteine(SAC) on the Proliferation of Umbilical Cord Blood(UCB)-derived Mesenchymal Stem Cells(MSCs)


To improve the growth of human mesenchymal stem cells(hMSCs) under general cell culture conditions(20% $O_2$ and 5% $CO_2$), we examined the effect of s-allylcysteine(SAC), which is known as an antioxidant and the main component of aged-garlic extract, on hydrogen peroxide-induced cellular stress in hMSCs. We found that SAC blocked hydrogen peroxideinduced cell death and cellular apoptosis, but that SAC did not improve the growth of hMSCs during short-term culture. To evaluate the protective effect of SAC, we examined the endogenous expression of the antioxidant enzymes catalase (CAT), superoxide dismutase(SOD), and glutathione peroxidase(Gpx) in hMSCs. Hydrogen peroxide was found to downregulate the expression of CAT, SOD, and Gpx at the protein level. However, in the pre-treatment group of SAC, SAC inhibited the hydrogen peroxide-induced down-regulation of CAT, SOD, and Gpx. Unfortunately, treatment with SAC alone did not induce the up-regulation of antioxidant enzymes and the cell proliferation of hMSCs. Surprisingly, SAC improved cell growth in a single cell level culture of hMSCs. These results indicate that SAC may be involved in the preservation of the self-renewal capacity of hMSCs. Taken together, SAC improves the proliferation of hMSCs via inhibition of oxidative-stress-induced cell apoptosis through regulation of antioxidant enzymes. In conclusion, SAC may be an indispensable component in an in vitro culture system of human MSCs for maintaining self-renewal and multipotent characterization of human MSCs.

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참고문헌 (12)

  1. Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y. 2001. Intake of garlic and its bioactive components. J Nutr 131:955S-962S 
  2. Kang TJ, Yeom JE, Lee HJ, Rho SH, Han H, Chae GT. 2004. Growth kinetics of human mesenchymal stem cells from bone marrow and umbilical cord blood. Acta Haematol 112:230-233 
  3. Nagatoshi I, Benjamin L. 2001. Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-kB activation. J Nutr 131:1020S-1026S 
  4. Zhu W, Chen J, Cong X, Hu S, Chen X. 2006. Hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells. Stem Cells 24:416-425 
  5. Agarwal KC. 1996. Therapeutic actions of garlic constituents. Med Res Rev 16:111-124 
  6. Augusti KT. 1996. Therapeutic values of onion(Allium cepa L.) and garlic(Allium sativum L.). Indian J Exp Biol 34:634-640 
  7. Zwaginga JJ, Doevendans P. 2003. Stem cell-derived angiogenic/vasculogenic cells: Possible therapies for tissue repair and tissue engineering. Clin Exp Pharmacol Physiol 30:900-908 
  8. Block E. 1985. The chemistry of garlic and onions. Sci Am 252:114-119 
  9. Kim YS, Park IK, Kwon JS, Jeong MH, Ahn YK. 2009. Approaches for optimization of stem cells for therapy of cardiovascular diseases. Tissue Eng Regen Med 6:186-191 
  10. Shin CH, Ihm JH. 2008. Effects of s-allylcysteine on oxidative stress in streptozotocin-induced diabetic rats. J Korean Endocrinol 23:129-136 
  11. Kim SS, Lee SG, Moon SH, Kim JM, Lee SH, Chung HM. 2008. Developmental pattern of human embryonic stem cells in optimized hypoxic culture condition. Tissue Eng Regen Med 5:474-481 
  12. Li T, Ito K, Sumi S-i, Fuwa T, Horie T. 2005. Antiapoptosis action of aged garlic extract(AGE) protects epithelial cells from methotrexate induced injury. Gut 54:1819-1820 

이 논문을 인용한 문헌 (2)

  1. Park, Ran-Sook 2011. "Effect of Oxygen Concentration, Physical Trauma on Proliferation of Umbilical Cord Blood-derived Mesenchymal Stem Cells" 한국식품영양학회지 = The Korean journal of food and nutrition, 24(4): 803~807 
  2. Park, Ran-Sook 2012. "Gene Profile of Mesenchymal Stem Cell Induced by SAC or Hydrogen Peroxide (H2O2)" 한국식품영양학회지 = The Korean journal of food and nutrition, 25(4): 863~870 


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