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사염화탄소로 섬유화가 유도된 흰쥐 간에서 털부처꽃 뿌리 추출물의 항산화 및 섬유화저해 활성

Antioxidant and Anti-fibrotic Properties of Root Extract of Lythrumsalicaria L. in $CCL_4$-Induced Liver Fibrosis Rat Model

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.17 no.4, 2009년, pp.243 - 250  

이승은 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  김금숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  한희선 (국립식량과학원) ,  서진숙 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  정해영 (부산대학교 약학대학) ,  박충범 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  박호기 (농촌진흥청 국립원예특작과학원 인삼특작부) ,  성낙술 ((재)금산국제인삼약초연구센터)

Abstract AI-Helper 아이콘AI-Helper

Fifty percent ethanol extract of Lythrum salicaria Linne root (LSR) was tested in vitro on antioxidant activity, and furthermore was investigated on antioxidative and fibrosis protecting activities in $CCL_4$-induced liver fibrosis rat model. Ratio of hepatic GSH/GSSG (reduced glutathione...

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제안 방법

  • plus LSR-treated groups (three groups), CCL4 plus silymarin-treated groups (positive controls, three groups). All groups were accommodated at 23~24℃, 55% humidity, and were maintained on 12 hours dark/12 hours light rule, were administrated with commercial stock diets (Samtaco, Korea) and were allowed of tap water for six weeks.
  • The cell viability test in SH-SY5Y treated with LSR explains that antioxidant activity of LSR mentioned above has not been arisen from cell toxicity or cell death. Next experiments were conducted to verify the antioxidant and hepatoprotective activity of LSR in liver fibrosis induced rat model.
  • , 2009). The study was performed to verify the activities of L. salicaria root extract against antioxidant and liver fibrosis-protection in rat fibrosis model.

대상 데이터

  • 20060101030022) of RDA, Republic of Korea. We thank Bio-Green21 project (No. 20050301034393 & 20070301034045) of RDA for providing the plant material used for this study.

데이터처리

  • The significance of data was analyzed with Duncan's multiple range test of one way ANOVA test using SAS program (Enterprise Guide 4) at p < 0.05.

이론/모형

  • (2006) and analyzed on the fluorescence at Ext485 nm/ Emm535 for 15 min. Effect of LSR on SH-SY5Y cells viability was analyzed by the modified method of Schmid et al. (2007) using MTT reagent.
  • GST activity in liver cytosol fraction was analyzed according to the principle of Habig et al. (1974) using the molecular extinction coefficient (EnM/340 nm = 9.6 nM−1 ㎝−1) of GSH-2,4-dinitrochlorobenzene (DNCB) conjugate produced from the reaction of DNCB and reduced glutathione (GSH) at 25℃ for 20 min.
  • , 2006). Peroxynitrite scavenging activity of LSR was conducted by the method of Kooy et al. (1994). Effect of LSR on BHPinduced ROS production in YPEN1 cells was evaluated in DMEM media by the modified method of Kweon et al.
  • Protein quantification for showing GST activity was conducted by the method of Bradford [1976] using bovine serum albumin as standard.
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