[논문]Protective Effect of Extracts from Euryale ferox against Glutamate-induced Cytotoxicity in Neuronal Cells

Lee, Mi-Ra; Kim, Ji-Hyun; Son, Eun-Soon; Park, Hae-Ryong

Protective Effect of Extracts from Euryale ferox against Glutamate-induced Cytotoxicity in Neuronal Cells 원문보기

Natural product sciences, v.15 no.3, 2009년, pp.162 - 166

Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University) , Kim, Ji-Hyun (Department of Food Science and Biotechnology, Kyungnam University) , Son, Eun-Soon (Department of Food Science and Biotechnology, Kyungnam University) , Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)

Abstract ▼ AI-Helper

Oxczaasssaidative stress plays an important role in neuronal cell death, which is associated with neurodegenerative conditions such as Alzheimer's and Parkinson's disease. This study evaluated the neuroprotective effect of Euryale ferox (EF) extracts against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. Specifically, neuroprotective effects of methanol and ethanol extracts were evaluated by the MTT reduction assay. The ethanol extracts of EF displayed dose dependent protection against neuronal cell death induced by 20 mM of glutamate. Furthermore, the ethanol extracts of EF was sequentially fractionated with hexane, diethyl ether, ethyl acetate, and water layer according to degree of polarity. The hexane fractions exhibited neuroprotective effect against glutamate-stressed N18-RE-105 cells. Overall, results suggest that EF extracts can potentially be used as chemotherapeutic agents against neuronal diseases.

주제어

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문제 정의

As mentioned previously, glutamate is the most abundant excitatory neurotransmitter in the CNS and linked to development of neurodegenerative disorders. This study demonstrates that GSH plays an important role in modulating glutamate-induced oxidative damage in neuronal cells. It has been proposed that high concentration of glutamate inhibits cystine uptake, resulting in the loss of intracellular GSH (Wang et al.

제안 방법

After MTT reduction assay, the MTT reduction rate (means ± S.D. of triplicate determination) were calculated by setting each of control survival rate.
In this study, we screened 435 varieties of herbal medicines and determined that Euryale ferox (EF) extracts exhibited neuroprotective effects against glutamate-induced cytotoxicity in N18-RE-105 cells. EF is edible and fried seeds or puffs are extremely nutritious (Das et al.
Lactate Dehydrogenase (LDH) Release Assay − Cytotoxicity was determined by measuring the release of lactate dehydrogenase (LDH).
The purpose of this research was to conduct a baseline study where the neuroprotective effects of EF extracts are examined against glutamate-stressed N18-RE-105 cells.
The Protective Effect of Hexane Fractions from EFE on Glutamate-induced Cytotoxicity − EFE, which showed the strongest neuroprotective effect, was further fractionated sequentially with hexane, diethyl ether, and ethyl acetate according to degree of polarity. We next examined the effects of EFE fractions on glutamate-stressed N18-RE-105 cells, which were determined by measuring the MTT reduction assay. As showed Fig.

데이터처리

Data were analyzed using the SPSS package for Windows (Version 14.0; Chicago, IL) and evaluated by one-way analysis of variance (ANOVA) followed by Scheffe's test.

성능/효과

During the present investigation, the seeds are also of great traditional medicinal value. In conclusion, extracts from EF could ameliorate glutamate-induced oxidative stress, which provided higher viability to N18-RE-105 cells. Additionally, we have provided evidence which appear to support the potential therapeutic benefit of EF in the treatment of neuropathological conditions.

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