Wee, Jae-Joon
(Ginseng Research Institute, Korea Ginseng Corporation)
,
Kim, Young-Sook
(Ginseng Research Institute, Korea Ginseng Corporation)
,
Kyung, Jong-Soo
(Ginseng Research Institute, Korea Ginseng Corporation)
,
Song, Yong-Bum
(Ginseng Research Institute, Korea Ginseng Corporation)
,
Do, Jae-Ho
(Ginseng Research Institute, Korea Ginseng Corporation)
,
Kim, Dong-Chung
(Department of Human Nutrition and Food Science, Chungwoon University)
,
Lee, Sung-Dong
(Department of Food and Nutrition, Korea University College of Health Science)
In this study, the anticoagulant compounds in Korean red ginseng (KRG) were investigated. KRG powder was extracted using hot methanol, and the methanol extract was fractionated into n-hexane, ethylacetate, n-butanol, and aqueous fractions by solvent partitioning. The remains from the methanol extrac...
In this study, the anticoagulant compounds in Korean red ginseng (KRG) were investigated. KRG powder was extracted using hot methanol, and the methanol extract was fractionated into n-hexane, ethylacetate, n-butanol, and aqueous fractions by solvent partitioning. The remains from the methanol extraction were further extracted with water and then dialyzed to obtain low and high molecular weight fractions. The anticoagulant activities of the seven fractions were evaluated in terms of thrombin time, prothrombin time, and activated partial thromboplastin time. Among these fractions, the ethylacetate fraction showed the most potent anticoagulant activity. The active components in the ethylacetate fraction were identified as the phenolic compounds vanillic, caffeic, ferulic, and p-coumaric acid via TLC and HPLC. These findings suggest that the anticoagulant activities of phenolic compounds contribute to the cardiovascular effects of KRG.
In this study, the anticoagulant compounds in Korean red ginseng (KRG) were investigated. KRG powder was extracted using hot methanol, and the methanol extract was fractionated into n-hexane, ethylacetate, n-butanol, and aqueous fractions by solvent partitioning. The remains from the methanol extraction were further extracted with water and then dialyzed to obtain low and high molecular weight fractions. The anticoagulant activities of the seven fractions were evaluated in terms of thrombin time, prothrombin time, and activated partial thromboplastin time. Among these fractions, the ethylacetate fraction showed the most potent anticoagulant activity. The active components in the ethylacetate fraction were identified as the phenolic compounds vanillic, caffeic, ferulic, and p-coumaric acid via TLC and HPLC. These findings suggest that the anticoagulant activities of phenolic compounds contribute to the cardiovascular effects of KRG.
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제안 방법
Silica gel TLC was performed to identify the active components. The plate was developed with CHCl3/ MeOH/H2O (65:35:10, v/v), 0.
This result indicates that saponins do not contribute to the anticoagulation activity of the EtOAc fraction. To confirm this, we prepared PPD and PPT saponin fractions, and examined their anticoagulation activities (Table 3). As shown in Table 3, the PPD and PPT fractions did not show anticoagulation activity.
To measure the thrombin time (TT), 50 μL of 0.02 M CaCl2, 50 μL of thrombin, and 50 μL of each fraction were preincubated at 37℃ for 3 minutes.
대상 데이터
KRG powder (Jungkwanjang) was provided by Korea Ginseng Corporation (Daejeon, Korea). Citrated human plasma was obtained from the Red Cross Blood Center (Daejeon, Korea).
성능/효과
In conclusion, the phenolic compounds in KRG have potent anticoagulant activity, whereas the saponin fractions, which were previously shown to possess antiplatelet aggregation activity, do not. Taken together, these results suggest that both saponins and phenolic compounds contribute to the cardiovascular effects of KRG through their antiplatelet aggregation and anticoagulant activities, respectively.
참고문헌 (25)
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