초록

탈지 미강으로부터 미강단백질을 추출하고 상업용 단백분해 효소로 가수분해하고 한외여과하여 얻어진 미강단백질 가수분해물을 Sephadex G-15로 분리하여 얻어진 peptide fraction에 칼슘, 철분을 binding하여 칼슘, 철분 함유 peptide를 제조하였다. 추출된 탈지 미강 단백질의 분자량은 10~66 kDa에 분포하고 있었다. 추출된 단백질을 Flavourzyme으로 가수분해 시, 최적 가수분해 시간은 6시간이었으며, 5kDa 이하로 한외여과 하여 얻어진 peptide를 Sephadex G-15로 분획한 결과 4개의 major peak를 얻었는데, 각 fraction의 칼슘, 철분을 binding한 결과 Ca/peptide는 FI에서, Fe/peptide는 F2에서 가장 많은 함량을 나타내었다. 본 연구 결과 얻어진 칼슘, 철분 binding peptide는 biomineral 기능성 식품의 소재로써 식품산업에 활용될 수 있다고 판단된다.

Abstract

Calcium and iron binding peptides were prepared by enzymatic hydrolysis and ultrafiltration of rice bran protein (RBP), which was isolated from defatted rice bran by phytase and xylanase treatment and ultrasonication. The isolated RBP had a molecular weight in the range of 10-66 kDa. The extracted proteins were hydrolyzed using Flavourzyme for 6 hr. After ultrafiltration under 5 kDa as molecular weight, the peptides were fractionated into 4 peaks by Sephadex G-15 gel permeation chromatography, and each fraction was determined for calcium and iron binding activity. As the result, Fl and F2 fractions were the best candidate for calcium and iron chelation, respectively. These results suggest that the calcium and iron binding peptides can be used as functional food additives in food industry.

주제어

#calcium-binding peptides   #defatted rice bran protein   #hydrolysates   #iron-binding peptides  

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