Abstract

Twelve Suffolk${\times}$Small-tail-Han male sheep (body weight 21-26 kg), aged four months, were used to study the effects of volatile fatty acids (VFA) on IGF-I (insulin-like growth factor-I), IGFBP-3 (insulin-like growth factor binding protein-3), GH (growth hormone), insulin and glucagon in plasma, and IGF-I and IGFBP-3 in different tissues. The sheep were randomly divided into four groups with 3 sheep in each group. The sheep were sustained by total intragastric infusions and four levels of mixed VFA (the molar proportion of acetic acid, propionic acid and butyric acid was 65:25:10), which supplied 333, 378, 423 and 468 KJ energy/kg $W^{0.75}$/d, were infused into the rumen as experimental Treatments I, II, III and IV, respectively. The experiment lasted 12 days, of which the first 8 days were for pretreatment and the last 4 days for collection of samples. At the end of the experiment, blood samples were taken and then the sheep were slaughtered and tissue samples from the rumen ventral sac, rumen dorsal sac, liver, duodenum and Longissimus dorsi muscle were obtained. IGF-I, IGFBP-3, GH, insulin and glucagon in plasma and IGF-I and IGFBP-3 in different tissues were analysed. Results showed that the concentration of IGF-I, IGFBP-3, GH, insulin or glucagon in plasma and the content of IGF-I and IGFBP-3 in the rumen dorsal sac, rumen ventral sac, liver or Longissimus dorsi muscle were increased with VFA infusion level (p0.05). It was concluded that IGF-I, IGFBP-3, GH, insulin and glucagon in plasma and IGF-I and IGFBP-3 in rumen dorsal sac, rumen ventral sac, liver and Longissimus dorsi muscle were increased significantly with increasing level of ruminal infusion of mixed VFA.

주제어

#Volatile Fatty Acids   #IGF-I   #IGFBP-3   #Growth Hormone   #Insulin   #Glucagon   #Sheep  

참고문헌 (26)

1. Bergman, E. N. 1990. Energy contributions of volatile fatty acids from the gastrointestinal tract in various species. Physiol. Rev. 70:567-590 
2. Coleman, M. E. and T. D. Etherton. 1991. Effects of exogenous porcine growth hormone on serum insulin-like growth factor binding proteins in growing pigs. J. Endocrinol. 128:175-180 
3. Kruth, J. 1982. Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol. Cell Biochem. 42:65-82 
4. MacLeod, N. A., W. Corrigall, R. A. Stirton and E. R. Orskov. 1982. Intragastric infusion of nutrients in cattle. Br. J. Nutr. 47:547-552 
5. McGuire, M. A., J. L. Vicini, D. E. Bauman and J. J. Veenhuizen. 1992. Insulin-like growth factors and binding proteins in ruminants and their nutritional regulation. J. Anim. Sci. 70:2901-2910 
6. Orskov, E. R., D. A. Grubb, J. S. Smith, A. J. F. Webster and W. Corrigall. 1979b. Efficiency of utilization of volatile fatty acids for maintenance and energy retention in sheep. Br. J. Nutr. 41:541-551 
7. Simon, C. J., L. E. Young, A. C. Underwood and D. R. Clemmons. 1992. Effects of recombinant insulin-like growth factor-I (IGFI) and growth hormone on plasma IGF-binding proteins in calorically restricted adults. J. Clin. Endocrinol. Metab. 76:603-608 
8. Sun, Y.-B. and G.-Y. Zhao. 2009. The relationship between the volatile fatty acids supply and the nitrogen retention in growing sheep nourished by total intragastric infusions. Small Rumin. Res. 81:8-12 
9. Vandehaar, M. J., B. K. Sharma and R. L. Fogwell. 1995. Effect of dietary energy restriction on the expression of insulin-like growth factor-I in liver and corpus luteum of heifers. J. Dairy Sci. 78:832-841 
10. Lane, M. A. and B. W. Jesse. 1997. Effect of volatile fatty acid infusion on development of the rumen epithelium in neonatal sheep. J. Dairy Sci. 80:740-746 
11. Cordano, P., H. M. Hammon, C. Morel, A. Zurbriggen and J. W. Blum. 2000. mRNA of insulin-like growth factor (IGF) quantification and presence of IGF binding proteins, and receptors for growth hormone, IGF-I and insulin, determined by reverse transcribed polymerase chain reaction, in the liver of growing and mature male cattle. Domest. Anim. Endocrinol. 19:191-203 
12. Sakata, T. and H. Tamate. 1979. Rumen epithelium cell proliferation accelerated by propionate and acetate. J. Dairy Sci. 62:49-50 
13. Neogrady, S., P. Galfi and F. Kutas. 1989b. Effect of intraruminal butyrate on the plasma insulin level in sheep. Acta Eterinaria Hungaria. 37:247-253 
14. Coleman, M. E., L. Russell and T. D. Etherton. 1994. Porcine somatotropin (pST) increases IGF-I mRNA abundance in liver and subcutaneous adipose tissue but not in skeletal muscle of growing pigs. J. Anim. Sci. 72:918-924 
15. Weller, P. A., M. J. Dauncey, P. C. Bates, J. M. Brameld, P. J. Buttery and R. S. Gilmour. 1994. Regulation of porcine insulin-like growth factor I and growth hormone receptor mRNA expression by energy status. Am. J. Physiol. Endocrinol. Metab. 266:E776-E785 
16. Cohick, W. S., M. A. McGuire, D. R. Clemmons and D. E. Bauman. 1992. Regulation of insulin-like growth factorbinding proteins in serum and lymph of lactating cows by somatotropin. J. Endocrinol. 130:1508-1514 
17. Orskov, E. R., D. A. Grubb, W. Wenham and W. Corrigall. 1979a. The sustenance of growing and fattening ruminants by intragastric infusion of volatile fatty acids and protein. Br. J. Nutr. 41:553-558 
18. Shen Z. M., H.-M. Seyfert, B. Loehrke, F. Schneider, R. Zitnan, A. Chudy, S. Kula, H. M. Hammon, J. W. Blum, H. Martens, H. Hagemeister and J. Voigt. 2004. An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats. J. Nutr. 134:11-17 
19. Kobayashi, S., H. Nogami and T. Ikeda. 1995. Growth hormone and nutrition interact to regulate expressions of kidney IGF-I and IGFBP mRNAs. Kidney Int. 48:65-71 
20. Neogrady, S., P. Galfi and F. Kutas. 1989a. Effects of butyrate and insulin and their interaction on the DNA synthesis of rumen epithelial cells in culture. Experimentia 45:94-96 
21. Baldwin, R. L. 1999. The proliferative actions of insulin, insulinlike growth factor-I, epidermal growth factor, butyrate and propionate on ruminal epithelial cells in vitro. Small Rumin. Res. 32:261-268 
22. Smith, J. W., L. E. Underwood and D. R. Clemmons. 1995. Effects of caloric or protein restriction on insulin-like growth factor-I (IGF-I) and IGF-binding proteins in children and adults. J. Clin. Endrocrinol. Metab. 80:443-449 
23. Breier, B. H., B. W. Gallaher and P. D. Gluckman. 1991. Radioimmunoassay for insulin-like growth factor-I: solutions to some potential problems and pitfalls. J. Endocrinol. 128:347-357 
24. Orskov, E. R. and N. A. Macleod. 1993. Effect of level of input of different proportions of volatile fatty acids on energy utilization in growing ruminants. Br. J. Nutr. 70:679-687 
25. Sano, H., S. Hayakawa, H. Takahashi and Y. Terashima. 1995. Plasma insulin and glucagon responses to propionate infusion into femoral and mesenteric veins in sheep. J. Anim. Sci. 73:191-197 
26. Schroeder, G. F., E. C. Titgemeyer, M. S. Awawdeh, J. S. Smith and D. P. Gnad. 2006. Effects of energy level on methionine utilization by growing steers. J. Anim. Sci. 84:1497-1503 

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