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NTIS 바로가기Development & reproduction = 발생과 생식, v.14 no.4, 2010년, pp.215 - 223
The construction of transgenic mouse using embryonic stem (ES) cells has been crucial in the functional studies of gene on mouse genome. Gene knockout mice have been powerful for elucidating the function of genes as well as a research model for human diseases. Gene targeting and gene trapping mathod...
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핵심어 | 질문 | 논문에서 추출한 답변 |
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유전자 함정법이란 무엇인가? | 유전자 기능에 관한 연구의 가장 효율적인 방법은 해당 유전자를 파괴하고 전체 개체에서 표현형을 관찰하는 것이다. 유전자 적중법의 대체 기술로 개발된 유전자 함정법은 매우 효율적이면서도 무작위적인 돌연변이 유발 기법이다(Gossler et al., 1987; Kothary et al. | |
유전자 파괴(녹아웃) 생쥐를 제조하는 두가지 주된 방법은 무엇이 있는가? | 이처럼 유전자 파괴(녹아웃) 생쥐를 제조하는 두 가지 주된 방법은 유전자 적중법과 유전자 함정법을 사용하는 것이다. 이런 면에서 생쥐 유전자의 연구는 사람 유전체 연구의 가장 완벽한 모델로서, 유전자가 파괴된 생쥐는 사람 질병의 좋은 모델이 되어 왔다(Austin et al. | |
전기천공법으로 녹아웃(적중)벡터를 ES세포에 삽입하면 어떤 현상이 생기는가? | , 1988). 전기천공법(electro-poratin)으로 이 벡터를 ES 세포에 삽입하면 표적벡터와 ES 세포 유전체에 있는 동일한 서열 사이에 상동재조합이 일어나 표적유전자의 서열 일부는 Neo로 교체된다. 상동재조합으로 생성된 Neo 저항성 ES 세포만이 살아남고, 그리고 TK는 GANC(gancyclovir) 존재하에 무작위 삽입된 클론을 제거한다(Cappechi, 1989). |
Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki M, Miura K, Araki M, Yamamura K (1999) Exchangeable gene trap using the Cre/mutated lox system. Cell Mol Biol 45:737-750.
Austin CP, Battey JF, Bradley A, Bucan M, Capecchi M, Collins FS, Dove WF, Duyk G, Dymecki S, Eppig JT, Grieder FB, Heintz N, Hicks G, Insel TR, oyner A, Koller BH, Lloyd KC, Magnuson T, Moore MW, Nagy A, Pollock JD, Roses AD, Sands AT, Seed B, Skarnes WC, Snoddy J, Soriano P, Stewart DJ, tewart F, Stillman B, Varmus H, Varticovski L, Verma IM, Vogt TF, von Melchner H, Witkowski J, Woychik RP, Wurst W, Yancopoulos GD, Young SG, Zambrowicz B (2004) The knockout mouse project. Nat Genet 36:921-924.
Bestor TH (2005) Transposons reanimated in mice. Cell 122:322-325.
Brown SD, Hancock JM (2006) The mouse genome. Genome Dyn 2:33-45.
Capecchi MR (1989) Altering the genome by homologous recombination. Science 244:1288-1292.
Capecchi MR (2001) Generating mice with targeted mutations. Nat Med 7:1086-1090.
Carlson CM, Frandsen JL, Kirchhof N, McIvor RS, Largaespada DA (2005) Somatic integration of an oncogene- harboring sleeping beauty transposon models liver tumor development in the mouse. Proc Natl Acad Sci USA 102:17059-17064.
Copeland NG, Jenkins NA, Court DL (2001) Recombineering: A powerful new tool for mouse functional genomics. Nat Rev Genet 2:769-779.
Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T (2005) Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 122:473-483.
Dupuy AJ, Fritz S, Largaespada DA (2001) Transposition and gene disruption in the male germline of the mouse. Genesis 30:82-88.
Friedel RH, Plump A, Lu X, Spilker K, Jolicoeur C, Wong K, Venkatesh TR, Yaron A, Hynes M, Chen B, Okada A, McConnell SK, Rayburn H, Tessier-Lavigne M (2005) Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes. Proc Natl Acad Sci USA 102:13188-13193.
Friedrich G, Soriano P (1991) Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes & Development 5: 1513-1523.
Gossler A, Joyner AL, Rossant J, Skarnes WC (1989) Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244:463-465
Glaser S, Anastassiadis K, Stewart AF (2005). Current issues in mouse genome engineering. Nat Genet 37:1187-1193.
Hacking DF (2008) "Knock, and it shall be opened": Knocking out and knocking in to reveal mechanisms of disease and novel therapies. Early Hum Dev 84:821-827.
Henikoff S, Till BJ, Comai L (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiol 135:630-636.
Ishida Y, Leder P (1999) RET: A poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Nucleic Acids Res 27:e35.
Ivics Z, Izsvak Z (2004) Transposable elements for transgenesis and insertional mutagenesis in vertebrates: A contemporary review of experimental strategies. Methods Mol Biol 260:255-276.
Kang HM, Kang NK, Kim DG, Shin HS (1997) Dicistronic tagging of genes active in embryonic stem cells of mice. Mol Cells 7: 502-508.
Kothary R, Clapoff S, Brown A, Campbell R, Peterson A, Rossant J (1988) A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube. Nature 335:435-437.
Lee T, Shah C, Xu EY (2007) Gene trap mutagenesis: A functional genomics approach towards reproductive research. Mol Hum Reprod 13:771-779.
Liu P, Jenkins NA, Copeland NG (2003) A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res 13:476-484.
Mansour SL, Thomas KR, Capecchi MR (1988) Disruption of the protooncogene int-2 in mouse embryoderived stem cells: A general strategy for targeting mutations to non-selectable genes. Nature 336:348-352.
McCarrick JW III, Parnes JR, Seong RH, Solter D, Knowles BB (1993) Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2:183-190.
Michael SK, Brennan J, Robertson EJ (1999) Efficient gene-specific expression of cre recombinase in the mouse embryo by targeted insertion of a novel IRES-Cre cassette into endogenous loci. Mech Dev 85:35-37.
Nagy A, Mar L, Watts G (2009) Creation and use of a cre recombinase transgenic database. Methods Mol Biol 530:1-4.
Niwa H (2010) Mouse ES cell cuture system as a model of development. Develop Growth Differ 52:275-283.
Niwa H, Araki K, Kimura S, Taniguchi S, Wakasugi S, Yamamura K (1993) An efficient gene-trap method using poly A trap vectors and characterization of gene-trap events. J Biochem 113:343-349.
O'Kane CJ, Gehring WJ (1987) Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci USA 84:9123-9127.
Schnutgen F, Ghyselinck NB (2007) Adopting the good reFLEXes when generating conditional alterations in the mouse genome. Transgenic Res 16:405-413.
Schnutgen F, Hansen J, De-Zolt S, Horn C, Lutz M, Floss T, Wurst W, Noppinger PR, von Melchner H (2008) Enhanced gene trapping in mouse embryonic stem cells. Nucleic Acids Res 36:e133.
Shigeoka T, Kawaichi M, Ishida Y (2005) Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells. Nucleic Acids Res 33:e20.
Skarnes WC, Auerbach A, Joiner AL (1992) A gene trap approach in mouse embryonic stem cells: The lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes & Development 6:903-918.
Sun LV, Jin K, Liu Y, Yang W, Xie X, Ye L, Wang L, Zhu L, Ding S, Su Y, Zhou J, Han M, Zhuang Y, Xu T, Wu X, Gu N, Zhong Y (2008) PBmice: An integrated database system of piggyBac (PB) insertional mutations and their characterizations in mice. Nucleic Acids Res 36:D729-734.
Szybalski W (1992) Use of the HPRT gene and the HAT selection technique in DNA-mediated transformation of mammalian cells: First steps toward developing hybridoma techniques and gene therapy. Bioessays 14: 495-500.
Testa G, Schaft J, van der Hoeven F, Glaser S, Anastassiadis K, Zhang Y, Hermann T, Stremmel W, Stewart AF (2004) A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles. Genesis 38:151-158.
Thomas KR, Capecchi MR (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51:503-512.
Vivian JL, Chen Y, Yee D, Schneider E, Magnuson T (2002) An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl- N-nitrosourea-mutagenized mouse embryonic stem cells. Proc Natl Acad Sci USA 99:15542-15547.
Wang W, Bradley A, Huang Y (2009) A piggyBac transposon- based genome-wide library of insertionally mutated Blm-deficient murine ES cells. Genome Res 19:667-673.
Wu S, Ying G, Wu Q, Capecchi MR (2007) Toward simpler and faster genome-wide mutagenesis in mice. Nat Genet 39:922-930.
Yagi T, Nada S, Watanabe N, Tamemoto H, Kohmura N, Ikawa Y, Aizawa S (1993) A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene. Anal Biochem 214:77-86.
Zambrowicz BP, Friedrich GA (1998) Comprehensive mammalian genetics: History and future prospects of gene trapping in the mouse. Int J Dev Biol 42:1025-1036.
Zambrowicz BP, Friedrich GA, Buxton EC, Lilleberg SL, Person C, Sands AT (1998) Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells. Nature 392:608-611.
Zheng B, Mills AA, Bradley A (1999) A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice. Nucleic Acids Res 27:2354-2360.
Zijlstra M, Bix M, Simister NE, Loring JM, Raulet DH, Jaenisch R (1990) $\beta$ 2-microglobulin deficient mice lack $CD4^{-}8^{+}$ cytotoxic T cells. Nature 344: 742-746.
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