Saengmaeksan (SMS) is a well known Korean traditional herbal prescription, which consists of 3 different herbs, Radix Ophiopogonis, Radix Ginseng, and Fructus Schisandrae. SMS has been commonly used in various disease including respiratory system and cardiovascular System in Korea. The objective of ...
Saengmaeksan (SMS) is a well known Korean traditional herbal prescription, which consists of 3 different herbs, Radix Ophiopogonis, Radix Ginseng, and Fructus Schisandrae. SMS has been commonly used in various disease including respiratory system and cardiovascular System in Korea. The objective of this study is to find out the improving effects of SMS against Dextran Sulfate Sodium (DSS)-induced ulcerative colitis. SMS reduced clinical signs of DSS-induced colitis, including body weight loss, shorten colon length, and increased disease activity index. The results showed that SMS significantly inhibited the activation of nuclear factor-${\kappa}B$ p65 in the colon tissues of DSS-treated mice. In addition, we observed that result showed that the levels of IL-6 in plasma were increased in DSS treated group compared to those of the normal group, but these increased levels were reduced by administration with SMS. Taken together, these findings suggest that SMS has improving effects on DSS-induced ulcerative colitis, which may explain its beneficial effect in the regulation of chronic intestinal inflammation.
Saengmaeksan (SMS) is a well known Korean traditional herbal prescription, which consists of 3 different herbs, Radix Ophiopogonis, Radix Ginseng, and Fructus Schisandrae. SMS has been commonly used in various disease including respiratory system and cardiovascular System in Korea. The objective of this study is to find out the improving effects of SMS against Dextran Sulfate Sodium (DSS)-induced ulcerative colitis. SMS reduced clinical signs of DSS-induced colitis, including body weight loss, shorten colon length, and increased disease activity index. The results showed that SMS significantly inhibited the activation of nuclear factor-${\kappa}B$ p65 in the colon tissues of DSS-treated mice. In addition, we observed that result showed that the levels of IL-6 in plasma were increased in DSS treated group compared to those of the normal group, but these increased levels were reduced by administration with SMS. Taken together, these findings suggest that SMS has improving effects on DSS-induced ulcerative colitis, which may explain its beneficial effect in the regulation of chronic intestinal inflammation.
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문제 정의
, 1990). The aim of this study is to examine the the improving effects of SMS on the pathogenesis of DSS-induced colitis. The specific aims were as follows: To assay the effect of SMS on clinical signs including weight loss, colon length, diarrhea, and occult/gross bleeding; to investigate the effect of SMS on inflammatory-related gene expression in DSS-treated colon tissues.
가설 설정
SMS was administered orally at doses of 1 g/kg once a day for 7 days prior to 5% DSS supplement. (A) Body weight of mice was measured. (B) The colons were removed at day 7 after DSS treatment, and the colon lengths were measured.
제안 방법
The inhibitory effects of SMS on the intestines in DSS-induced experimental colitis were evaluated from the experiment. The physiological signs (weight loss, colon length, diarrhea, and occult/ gross bleeding) were observed by 5% DSS treatment for 7 days, and their DAIs were calculated. As shown in Fig.
The aim of this study is to examine the the improving effects of SMS on the pathogenesis of DSS-induced colitis. The specific aims were as follows: To assay the effect of SMS on clinical signs including weight loss, colon length, diarrhea, and occult/gross bleeding; to investigate the effect of SMS on inflammatory-related gene expression in DSS-treated colon tissues.
To investigate the effect of SMS on IL-6 levels in mouse plasma affected by colitis, ELISA was performed. At the end of the experiment, blood samples were obtained from mouse.
대상 데이터
Female BALB/c mice (6 weeks old) were obtained from the Da-Mool Science (Taejeon, Korea). Mice were housed in a specific pathogen-free environment for at least 1 week for adaptation to the environmental changes and were sacrificed by CO2 inhalation at the end of the study.
The SMS which is a mixture of three traditional drugs as shown in Table 1 was purchased from Daehak Oriental Pharmacy (Iksan, Korea). Extract of SMS was prepared by decocting the dried prescription with boiling distilled water.
데이터처리
of at least three experiments. The results were examined using independent t-tests and ANOVA with a Tukey post hoc test. A P value of < 0.
성능/효과
, 2010). In this study, we showed that the levels of IL-6 in plasma were increased in DSS treated group compared to those of the normal group, but these inductions were reduced by treatment with SMS. These results indicated that the anti-inflammatory activity of SMS is due to regulation of inflammatory mediators in DSS-induced colitis.
후속연구
Based on these results, we suggest that SMS may be a useful therapeutic candidate for colitis. However, the further studies must be performed to elucidate the precise mechanism of SMS for the treatment of intestinal inflammatory disorders.
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