[논문]Dolichos biflorus Linn attenuate progression of renal damage in alloxan-induced diabetic rats

Chidrewar, G.U.; Mane, V.S.; Ghaisas, M.M.; Deshpande, A.D.

doi:10.3742/opem.2010.10.2.134

Dolichos biflorus Linn attenuate progression of renal damage in alloxan-induced diabetic rats 원문보기

Oriental pharmacy and experimental medicine, v.10 no.2, 2010년, pp.134 - 140

Chidrewar, G.U. (Department of Pharmacology, Padm. Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research) , Mane, V.S. (Department of Pharmacology, Padm. Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research) , Ghaisas, M.M. (Principal, Indira College of Pharnacy) , Deshpande, A.D. (Department of Pharmacology, Padm. Dr. D.Y. Patil Institute of Pharmaceutical Sciences and Research)

Abstract ▼ AI-Helper

Dolichos biflorus Linn. (Fabaceae), commonly known as Horse gram is a medicinal plant, used in folk medicine for treating kidney stones and diabetes mellitus. The purpose of the present study was to investigate the effects of daily oral feeding of various doses of methanolic extract of Dolichos biflorus seeds (DB) for 42 days on blood glucose concentrations and kidney functions in Alloxan-diabetic rats. Plasma glucose levels, body weight, serum creatinine, and urinary albumin levels were monitored on $15^{th}$, $29^{th}$, $43^{rd}$ day. Renal hypertrophy was assessed as the ratio between the kidney weight and body weight of the rats. Plasma glucose concentrations in Alloxan-diabetic rats were significantly reduced by the administration of DB (350 mg/kg) and DB (700 mg/kg) on day 15 and onwards (P < 0.01). After 15 days of Alloxan administration urinary albumin levels (UAE) were over 5 fold higher in diabetic controls as compared to normal controls. Treatment with DB significantly prevented the rise in UAE levels from day 15 to 43 in comparison to diabetic controls (P < 0.01). Renal hypertrophy was significantly higher in diabetic controls as compared to non-diabetic controls. Treatments with DB (350 mg/kg) and DB (700 mg/kg) significantly prevented renal hypertrophy (P < 0.01) as compared to diabetic controls. DB (175 mg/kg) failed to modify renal hypertrophy. Thus the present study indicates that methanolic extract of Dolichos biflorus may be useful in management of hyperglycemia and kidney functions in Alloxan-diabetic rats.

주제어

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문제 정의

The primary objective of this study was to assess the efficacy of traditional anti-diabetic plant in diabetic nephropathy. Research on medicinal plants has increased recently all over the world.

대상 데이터

Wistar rats of either sex weighing 150 - 180 g were purchased from National Toxicology Center (NTC), Pune. The rats were housed in standard laboratory conditions with free access to standard pellet diet and water ad libitum.

데이터처리

Values are expressed as mean ± S.E.M. Statistical analysis was performed using one way ANOVA.

성능/효과

In conclusion, treatment with methanolic extract of DB [notably DB (350 mg/kg) and DB (700 mg/kg)] to diabetic rats prevented the increase in Serum creatinine, UAE excretion, and renal hypertrophy as well as caused reduction in plasma glucose levels. Treatment also halted the progression of weight loss.

후속연구

Hence the traditional claim of this plant is proved. However, further work is warranted to pinpoint the exact mechanism of nephroprotective effect of DB and to identify the active constituent (s) responsible for such an effect.

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