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논문 상세정보

재배와 야생 지치의 추출물과 용매별 분획물의 항산화효과

Antioxidant Activity of Solvent Fractions from Cultivated and Wild Gromwell

초록

식품의 색소원 및 약용으로 사용되어 오던 지치과 식물에 대한 정확한 품질지표를 마련하고자 재배방법이 다른 야생과 재배 지치에 대한 산화제거 물질과 관계되는 총 페놀 화합물 함량, 전자공여능, SOD 유사활성, hydroxy radical 소거능, lecithin oxidation 저해활성 등의 항산화능을 조사하였다. 지치의 methanol 추출물에 대한 총 페놀 함량은 야생지치 0.14%, 재배 지치 0.13%이었고 용매별 분획물에 있어서 가장 높은 활성을 나타낸 것은 야생 지치의 경우 ethyl acetate 분획물, 재배 지치의 경우 n-hexane 분획물이었다. 지치의 methanol 추출물에 있어서 DPPH radical 50% 저해효과인 $IC_{50}$은 야생 지치 794.41 ${\mu}g$/mL, 재배 지치 971.86 ${\mu}g$/mL로서 야생 지치가 재배 지치보다 적은 농도에서 높은 활성을 나타내었다(p<0.05). 또한 용매별 분획물의 $IC_{50}$에 있어, 야생 지치는 ethyl acetate 분획층, 재배 지치는 nhexane 분획물에서 가장 높은 활성을 보였다. 또한 야생과 재배 지치의 SOD 활성 효과는 10~500 ${\mu}g$/mL 농도에서 의존적인 활성을 나타내었고 특히 메탄올 추출물은 농도 500 ${\mu}g$/mL에서는 다른 용매 분획물보다도 높은 활성을 나타내었다. Hydroxy radical 소거 활성은 야생과 재배 지치모두 1,000 ${\mu}g$/mL 농도에서 chloroform 분획층과 n-hexane 분획층을 제외하고 50% 이하의 가장 낮은 활성을 나타내었다. 마지막으로 lecithin 산화저해 활성은 야생과 재배 지치의 메탄올과 용매별 분획물의 농도 200~1000 ${\mu}g$/mL에서 높은 활성을 나타내었고 특히 농도 1,000 ${\mu}g$/mL의 chloroform 층에서는 90% 내외의 높은 제어효과를 확인하였다. 이상의 결과로 야생과 재배 지치 모두 항산화성을 가지는 기능성식품으로서의 활용 가능성이 높을 것으로 분석되었다.

Abstract

In order to set up an accurate quality criteria for the Boraginaceae that have been traditionally used for medical purposes and food colorant, and to assess its viability as functional food ingredient, antioxidant tests were conducted on the wild and cultivated plants. Variety of indicators including total contents of phenol, DPPH, SOD-liked effect, hydroxy radical-scavenging effect, lecithin oxidation inhibitory effect, etc were analyzed. Wild and cultivated gromwell's total contents of phenol in their methanol extracts were 0.14% and 0.13%, while they were most active in ethyl acetate extracts and n-hexane extracts, respectively. $IC_{50}$ values of methanol extract of the wild and cultivated plants were 794.41 ${\mu}g$/mL and 971.86 ${\mu}g$/mL, indicating that the wild plant is more responsive (p<0.05) to low concentration. Also the wild and cultivated plants were most active in ethyl acetate fraction and n-hexane extracts when their $IC_{50}$ values were measured by each solvent extracts. SOD-liked effects of both plants were concentration dependent while methanol extracts were more active (p<0.05) in 500 ${\mu}g$/mL than other solvent extracts. Hydroxy radical-scavenging effect of both plants showed less than 50% activity in concentration of 1,000 ${\mu}g$/mL except in chloroform fraction and n-hexane fraction. Lecithin oxidation inhibitory effects of the wild and cultivated plants were active in methanol and solvent extracts of 200~1000 ${\mu}g$/mL. Especially it showed 90% of high inhibitory effect in 1,000 ${\mu}g$/mL of chloroform fraction. Hence, both wild and cultivated Boraginaceae were analyzed to be viable as functional food ingredient.

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이 논문을 인용한 문헌 (3)

  1. An, Tae Jin ; Shin, Kyu Seop ; Ahn, Young Sup ; Hur, Mok ; Park, Chung Berm 2013. "Identification of Fungal Pathogen Causing Seedling Rot of Lithospermum erythrorhizon and Study on the Optimum Growing Temperature for Decreasing of the Seedling Rot" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 21(1): 27~31 
  2. Jung, Min-Hwa ; Lee, Su-Seon ; Park, Si-Hyang ; Hwang, Hye-Jung 2013. "The Antioxidative Effect of Ethanol Extracts from Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium Linn, and Lonicera japonica" 생명과학회지 = Journal of life science, 23(5): 643~649 
  3. 2013. "" 韓國資源植物學會誌 = Korean journal of plant resources, 26(3): 403~409 

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