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잎담배 중 유리 아미노산 분석

The Analysis of Free Amino Acids in Tobacco


A simple and sensitive automatic amino acid analyzer method for the determination of free amino acids in tobacco was described. Sample preparation consisted of a single step of extraction with 0.1 mol HCl at ambient temperature in 60 min by sonication, followed by filtration of an aliquot. Automated amino acid analyzer was used to construct a post-column ninhydrin reaction unit to monitor amino acids separated by liquid chromatography using a series of eluting buffers. By optimization of sample preparation, separation of 19 amino acids was achieved. Limits of quantitation was 0.01 mg/g, coefficients of variation ranged from 0.5 % to 8.9 % and recoveries range from 85 % to 106 %. The method was applied to the analysis of amino acids contents of tobacco leaves in different varieties.

참고문헌 (17)

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  8. Laseter, J.L., Weete, J.D., Albert, A. and Walkinshaw, C.H. (1971) Gas chromatographic determination of trimethylsilyl derivatives of free amino acids from a botanical source, Analytical Letter 4: 671-681. 
  9. Moore, S. and Stein, W.H. (1948) Photometric ninhydrin method for use in the chromatography of amino acids, J. Biological Chem. 176: 367-388. 
  10. Noma, M., Noguchi, M. and Tamaki, E. (1973) Isolation and characterization of D-alanyl- D-alanine from tobacco leaves, Agr. Biol. Chem. 37: 2439. 
  11. Panasiuk, R., marowicz, R., Kostyra, H. and Sijtsma, L. (1998) Determination of α-amino nitrogen in pea protein hydrolysates: a comparison of three analytical methods, Food Chemistry 62: 36337. 
  12. Roth, M. (1971) Fluorescence reaction for amino acids, Anal. Chem. 43: 880-882. 
  13. Tso, T.C. (1991) Production, physiology, and biochemistry of tobacco plant, Ideals Inc., Beltsville, p 497. 
  14. Watanabe, Y. and Imai, K. (1981) Highperformance liquid chromatography and sensitive detection of amino acids derivatized with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole, Anal. Bioehem. 116:471-472. 
  15. Yang, S.S. and Smetena, I. Determination of free amino acids in tobacco by HPLC with fluorescence detection and precolumn derivatization. Chromatographia, 37: 593-98. 
  16. Zhu, X.L., Zhu, Q.S. and Gao Y. (2008) Fast analysis of free amino acids in tobacco by HPLC with fluorescence detection and automated derivatization, J. the Chinese Chem. Society, 55: 1094-1100. 
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